Blastopirellula cremea

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Blastopirellula

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5042 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368KRR4|A0A368KRR4_9BACT Uncharacterized protein OS=Blastopirellula cremea OX=1031537 GN=DTL42_17190 PE=4 SV=1
MM1 pKa = 7.6TDD3 pKa = 3.31YY4 pKa = 10.89NASDD8 pKa = 3.52VKK10 pKa = 10.13QQQVAYY16 pKa = 9.04TYY18 pKa = 11.41DD19 pKa = 3.32LFNRR23 pKa = 11.84LIGRR27 pKa = 11.84KK28 pKa = 9.07LDD30 pKa = 3.52SDD32 pKa = 3.89GDD34 pKa = 4.1GTVDD38 pKa = 3.46QSGTFIYY45 pKa = 10.85DD46 pKa = 3.14GDD48 pKa = 4.07QIMLQIDD55 pKa = 3.68DD56 pKa = 4.3NGEE59 pKa = 3.72VDD61 pKa = 5.81HH62 pKa = 6.96RR63 pKa = 11.84LLWRR67 pKa = 11.84ANVDD71 pKa = 3.33QLLADD76 pKa = 4.25EE77 pKa = 4.69NASGDD82 pKa = 4.19VYY84 pKa = 10.67WALTDD89 pKa = 3.67HH90 pKa = 6.9LNTVHH95 pKa = 7.44DD96 pKa = 3.77WAEE99 pKa = 4.02YY100 pKa = 11.03DD101 pKa = 4.59DD102 pKa = 5.03LTDD105 pKa = 3.33TTSVVNHH112 pKa = 6.62IAYY115 pKa = 9.68DD116 pKa = 3.38AFGNVLSEE124 pKa = 4.44TNATLDD130 pKa = 3.33TTGFGFTARR139 pKa = 11.84YY140 pKa = 8.89FDD142 pKa = 4.09EE143 pKa = 4.69ATGLQYY149 pKa = 9.88NTNRR153 pKa = 11.84WYY155 pKa = 10.58NAQLGRR161 pKa = 11.84WMSPDD166 pKa = 4.14PIGFEE171 pKa = 4.18AGDD174 pKa = 3.78EE175 pKa = 3.93NLYY178 pKa = 10.48RR179 pKa = 11.84YY180 pKa = 9.33VGNEE184 pKa = 3.54VTVFTDD190 pKa = 3.79PSGLEE195 pKa = 3.93EE196 pKa = 4.04

Molecular weight: 22.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368KQZ0|A0A368KQZ0_9BACT Uncharacterized protein OS=Blastopirellula cremea OX=1031537 GN=DTL42_12240 PE=4 SV=1
MM1 pKa = 7.19ATLAGVRR8 pKa = 11.84QRR10 pKa = 11.84LIEE13 pKa = 4.12SCLIMNLPILRR24 pKa = 11.84MRR26 pKa = 11.84IAGNLIACVIHH37 pKa = 6.31MYY39 pKa = 10.78VDD41 pKa = 3.82TLRR44 pKa = 11.84LGSQRR49 pKa = 11.84RR50 pKa = 11.84GLCTLTSHH58 pKa = 6.85GGGWHH63 pKa = 5.43WRR65 pKa = 11.84MKK67 pKa = 9.09PLCVPRR73 pKa = 11.84CLWPVPGKK81 pKa = 10.15QGHH84 pKa = 5.74AQPGNQAARR93 pKa = 11.84VRR95 pKa = 11.84LFSAALEE102 pKa = 4.55SVASLPLAVVDD113 pKa = 4.1FRR115 pKa = 11.84EE116 pKa = 4.8AKK118 pKa = 9.75RR119 pKa = 11.84HH120 pKa = 5.13PNPGKK125 pKa = 9.54MPGAPGVFAINVLLKK140 pKa = 10.43LVPFVSGLVFSAYY153 pKa = 9.87EE154 pKa = 3.86CLQLVKK160 pKa = 10.93

Molecular weight: 17.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5042

0

5042

1789957

29

4800

355.0

39.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.296 ± 0.037

1.102 ± 0.014

5.709 ± 0.027

6.596 ± 0.026

3.849 ± 0.018

7.365 ± 0.032

2.206 ± 0.015

5.356 ± 0.023

4.234 ± 0.03

10.005 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.017

3.443 ± 0.024

5.234 ± 0.024

4.548 ± 0.03

5.862 ± 0.031

6.149 ± 0.028

5.505 ± 0.031

7.004 ± 0.025

1.532 ± 0.013

2.671 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski