Capybara microvirus Cap3_SP_416
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W527|A0A4P8W527_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_416 OX=2585448 PE=4 SV=1
MM1 pKa = 7.13 FRR3 pKa = 11.84 KK4 pKa = 9.05 IQNFIPEE11 pKa = 4.15 PSSEE15 pKa = 3.61 IFYY18 pKa = 11.22 NEE20 pKa = 3.73 DD21 pKa = 3.03 EE22 pKa = 4.2 EE23 pKa = 6.38 RR24 pKa = 11.84 YY25 pKa = 10.51 VLVDD29 pKa = 3.74 DD30 pKa = 4.21 SPIRR34 pKa = 11.84 VISSTPIDD42 pKa = 3.76 ACNGKK47 pKa = 9.69 LPASIPHH54 pKa = 5.52 FQEE57 pKa = 4.08 FTLTTLLNAGVKK69 pKa = 10.08 LEE71 pKa = 3.89 QLNTVLLRR79 pKa = 11.84 PSVQQFEE86 pKa = 4.31 QSFTDD91 pKa = 5.16 FFTQTSNEE99 pKa = 4.05 FVNHH103 pKa = 5.77 YY104 pKa = 10.29 EE105 pKa = 4.01 SDD107 pKa = 3.91 NII109 pKa = 4.19
Molecular weight: 12.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.463
IPC2_protein 4.457
IPC_protein 4.329
Toseland 4.177
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.139
Rodwell 4.164
Grimsley 4.088
Solomon 4.266
Lehninger 4.215
Nozaki 4.393
DTASelect 4.507
Thurlkill 4.19
EMBOSS 4.164
Sillero 4.431
Patrickios 3.694
IPC_peptide 4.266
IPC2_peptide 4.431
IPC2.peptide.svr19 4.345
Protein with the highest isoelectric point:
>tr|A0A4V1FVT1|A0A4V1FVT1_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_416 OX=2585448 PE=4 SV=1
MM1 pKa = 7.82 CIAPLEE7 pKa = 4.51 IINPKK12 pKa = 9.91 LDD14 pKa = 3.67 PRR16 pKa = 11.84 STDD19 pKa = 3.21 PTLLEE24 pKa = 4.37 VGCGKK29 pKa = 10.51 CEE31 pKa = 3.68 EE32 pKa = 4.8 CRR34 pKa = 11.84 NQRR37 pKa = 11.84 KK38 pKa = 9.98 DD39 pKa = 2.88 EE40 pKa = 4.6 WIVRR44 pKa = 11.84 TYY46 pKa = 11.08 FQMLHH51 pKa = 4.95 NRR53 pKa = 11.84 EE54 pKa = 3.61 RR55 pKa = 11.84 GYY57 pKa = 11.54 YY58 pKa = 8.32 NTFFTLTYY66 pKa = 10.79 SDD68 pKa = 4.51 DD69 pKa = 4.2 CLPHH73 pKa = 8.15 LLIDD77 pKa = 4.56 GKK79 pKa = 10.27 MIPCFNKK86 pKa = 9.53 QDD88 pKa = 2.92 IFQFFKK94 pKa = 10.49 TLKK97 pKa = 8.22 KK98 pKa = 10.1 HH99 pKa = 5.09 LNRR102 pKa = 11.84 RR103 pKa = 11.84 YY104 pKa = 10.37 GINDD108 pKa = 3.18 TNYY111 pKa = 10.83 LVGCEE116 pKa = 4.39 YY117 pKa = 11.2 GDD119 pKa = 3.39 HH120 pKa = 5.59 TQRR123 pKa = 11.84 SHH125 pKa = 3.9 YY126 pKa = 10.07 HH127 pKa = 6.03 IMLGCPEE134 pKa = 5.05 HH135 pKa = 6.11 GTKK138 pKa = 10.31 EE139 pKa = 4.06 DD140 pKa = 4.18 LDD142 pKa = 4.15 PRR144 pKa = 11.84 DD145 pKa = 3.97 LFQFCKK151 pKa = 10.65 NYY153 pKa = 8.95 WKK155 pKa = 10.46 HH156 pKa = 6.63 GFMFPRR162 pKa = 11.84 VFEE165 pKa = 4.27 GGRR168 pKa = 11.84 DD169 pKa = 3.42 SHH171 pKa = 7.7 GYY173 pKa = 7.64 DD174 pKa = 3.19 HH175 pKa = 7.43 KK176 pKa = 10.96 PFLVTGDD183 pKa = 3.46 ILAPAQYY190 pKa = 9.87 ISKK193 pKa = 9.77 YY194 pKa = 7.76 VCKK197 pKa = 10.57 DD198 pKa = 2.99 LAFYY202 pKa = 9.55 DD203 pKa = 3.56 IPEE206 pKa = 4.26 ISSLSDD212 pKa = 3.1 RR213 pKa = 11.84 LDD215 pKa = 3.36 KK216 pKa = 11.7 EE217 pKa = 4.03 EE218 pKa = 3.87 FKK220 pKa = 10.78 KK221 pKa = 10.94 IKK223 pKa = 10.33 GSLPFLTVSRR233 pKa = 11.84 GFGLYY238 pKa = 10.47 LRR240 pKa = 11.84 DD241 pKa = 3.84 VIMKK245 pKa = 10.16 SDD247 pKa = 3.21 LHH249 pKa = 7.74 NIFKK253 pKa = 10.9 YY254 pKa = 10.18 GICFEE259 pKa = 4.6 GNPRR263 pKa = 11.84 RR264 pKa = 11.84 HH265 pKa = 6.71 KK266 pKa = 10.18 IPPYY270 pKa = 9.71 ILRR273 pKa = 11.84 KK274 pKa = 7.84 MCYY277 pKa = 9.93 DD278 pKa = 2.95 IDD280 pKa = 3.86 KK281 pKa = 10.47 RR282 pKa = 11.84 VVDD285 pKa = 4.6 GEE287 pKa = 4.53 SKK289 pKa = 10.97 VYY291 pKa = 9.67 WKK293 pKa = 10.87 LNEE296 pKa = 3.85 FGKK299 pKa = 10.27 QIRR302 pKa = 11.84 LQNLDD307 pKa = 3.88 NIINSTYY314 pKa = 8.97 TSLYY318 pKa = 10.63 KK319 pKa = 10.54 EE320 pKa = 4.12 FSFINLNSDD329 pKa = 3.59 YY330 pKa = 9.96 MRR332 pKa = 11.84 KK333 pKa = 9.19 FLSEE337 pKa = 3.83 YY338 pKa = 10.25 GYY340 pKa = 9.56 TSSSFRR346 pKa = 11.84 KK347 pKa = 10.51 YY348 pKa = 10.39 IDD350 pKa = 4.17 DD351 pKa = 4.45 LLDD354 pKa = 3.61 GRR356 pKa = 11.84 SFKK359 pKa = 11.05 DD360 pKa = 3.16 LAIYY364 pKa = 10.02 RR365 pKa = 11.84 YY366 pKa = 9.78 LYY368 pKa = 10.34 RR369 pKa = 11.84 NRR371 pKa = 11.84 ICLHH375 pKa = 5.68 EE376 pKa = 5.45 SGMVIEE382 pKa = 5.16 PGFVSPDD389 pKa = 2.98 SSLTEE394 pKa = 3.6 KK395 pKa = 10.4 RR396 pKa = 11.84 VYY398 pKa = 10.18 TRR400 pKa = 11.84 FFKK403 pKa = 9.87 HH404 pKa = 5.06 TLYY407 pKa = 10.85 NSSIPVMDD415 pKa = 4.09 IFRR418 pKa = 11.84 DD419 pKa = 3.73 HH420 pKa = 7.15 RR421 pKa = 11.84 GEE423 pKa = 4.15 KK424 pKa = 9.93 YY425 pKa = 10.26 KK426 pKa = 10.99 SSSFSKK432 pKa = 10.66 KK433 pKa = 10.42 FIQDD437 pKa = 3.76 TCCFTYY443 pKa = 10.86 NSLPLFSGFDD453 pKa = 5.41 LILDD457 pKa = 4.05 WYY459 pKa = 10.31 KK460 pKa = 11.09 RR461 pKa = 11.84 MSIFHH466 pKa = 6.54 KK467 pKa = 10.28 KK468 pKa = 9.79 YY469 pKa = 9.39 NLEE472 pKa = 3.97 HH473 pKa = 7.0 RR474 pKa = 11.84 SIQYY478 pKa = 9.85 RR479 pKa = 11.84 YY480 pKa = 10.37 YY481 pKa = 11.17 NYY483 pKa = 9.26 IHH485 pKa = 6.25 QKK487 pKa = 9.85 FVEE490 pKa = 4.44 HH491 pKa = 6.57 VPP493 pKa = 3.45
Molecular weight: 58.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.948
IPC2_protein 8.097
IPC_protein 7.907
Toseland 7.717
ProMoST 8.478
Dawson 8.653
Bjellqvist 8.887
Wikipedia 8.653
Rodwell 8.712
Grimsley 7.761
Solomon 8.712
Lehninger 8.697
Nozaki 8.975
DTASelect 8.668
Thurlkill 8.712
EMBOSS 8.829
Sillero 8.99
Patrickios 3.948
IPC_peptide 8.712
IPC2_peptide 7.775
IPC2.peptide.svr19 7.981
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1611
109
661
402.8
46.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.593 ± 1.737
1.614 ± 0.577
6.083 ± 0.456
5.276 ± 0.632
6.269 ± 0.71
5.028 ± 0.477
2.607 ± 0.514
6.021 ± 0.65
5.897 ± 1.033
9.497 ± 0.352
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.986 ± 0.24
6.642 ± 0.823
4.345 ± 0.47
4.904 ± 1.061
4.593 ± 0.855
9.249 ± 0.826
4.531 ± 0.395
4.718 ± 0.823
0.869 ± 0.139
5.276 ± 1.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here