Candidatus Kinetoplastibacterium blastocrithidii TCC012E

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Kinetoplastibacterium; Candidatus Kinetoplastibacterium blastocrithidii

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1MDJ1|M1MDJ1_9PROT tRNA-specific 2-thiouridylase MnmA OS=Candidatus Kinetoplastibacterium blastocrithidii TCC012E OX=1208922 GN=mnmA PE=3 SV=1
MM1 pKa = 7.7CFYY4 pKa = 10.92SQDD7 pKa = 3.57VLVDD11 pKa = 3.63ISTAAMDD18 pKa = 3.45TGGYY22 pKa = 10.4GVFLTITSDD31 pKa = 3.36SGSKK35 pKa = 9.73VDD37 pKa = 5.01NVFSYY42 pKa = 10.95LGDD45 pKa = 3.85CEE47 pKa = 4.39SLYY50 pKa = 10.83EE51 pKa = 4.11AVSLAEE57 pKa = 3.68IFANKK62 pKa = 9.23WIDD65 pKa = 3.43EE66 pKa = 4.08NLISNSS72 pKa = 3.27

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1LZK8|M1LZK8_9PROT ATP synthase subunit c OS=Candidatus Kinetoplastibacterium blastocrithidii TCC012E OX=1208922 GN=atpE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.17RR14 pKa = 11.84THH16 pKa = 5.95GFRR19 pKa = 11.84IRR21 pKa = 11.84MKK23 pKa = 9.01TRR25 pKa = 11.84SGRR28 pKa = 11.84AILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.68GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

723

0

723

240842

38

1394

333.1

37.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.732 ± 0.088

1.307 ± 0.028

5.796 ± 0.067

5.594 ± 0.078

4.366 ± 0.072

6.209 ± 0.078

1.934 ± 0.032

10.184 ± 0.104

6.949 ± 0.09

9.75 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.036

6.361 ± 0.082

3.289 ± 0.036

2.541 ± 0.046

4.733 ± 0.065

8.363 ± 0.069

4.299 ± 0.056

5.991 ± 0.073

0.92 ± 0.03

3.283 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski