Chayote yellow mosaic virus
Average proteome isoelectric point is 8.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O56817|O56817_9GEMI Transcriptional activator protein OS=Chayote yellow mosaic virus OX=222450 GN=C2 PE=3 SV=1
MM1 pKa = 7.71 APPKK5 pKa = 10.21 RR6 pKa = 11.84 FQINAKK12 pKa = 10.09 NYY14 pKa = 8.52 FLTYY18 pKa = 8.4 PQCSLTKK25 pKa = 10.58 EE26 pKa = 4.03 EE27 pKa = 5.05 ALSQLRR33 pKa = 11.84 SLRR36 pKa = 11.84 TPTNQKK42 pKa = 9.29 YY43 pKa = 10.4 IKK45 pKa = 9.9 VCRR48 pKa = 11.84 EE49 pKa = 3.77 LHH51 pKa = 6.95 EE52 pKa = 5.73 DD53 pKa = 3.93 GQPHH57 pKa = 6.38 LHH59 pKa = 6.88 ALIQFEE65 pKa = 4.94 GKK67 pKa = 9.96 YY68 pKa = 9.77 KK69 pKa = 10.46 CQNQRR74 pKa = 11.84 FFDD77 pKa = 4.04 LVSPTRR83 pKa = 11.84 STHH86 pKa = 4.99 FHH88 pKa = 6.94 PNVQGAKK95 pKa = 9.25 SCSDD99 pKa = 3.11 VKK101 pKa = 11.23 SYY103 pKa = 10.83 IEE105 pKa = 4.2 KK106 pKa = 10.82 DD107 pKa = 3.05 GDD109 pKa = 4.05 SIEE112 pKa = 4.08 WGEE115 pKa = 3.9 FQVDD119 pKa = 3.21 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGCHH128 pKa = 6.55 KK129 pKa = 11.07 ANDD132 pKa = 3.93 AAAEE136 pKa = 3.94 ALNAGSAEE144 pKa = 3.93 AALAIIKK151 pKa = 9.62 EE152 pKa = 4.4 KK153 pKa = 10.84 LPKK156 pKa = 10.24 DD157 pKa = 3.94 YY158 pKa = 10.67 IFQYY162 pKa = 11.18 HH163 pKa = 6.43 NIKK166 pKa = 10.91 ANLDD170 pKa = 3.91 KK171 pKa = 10.8 IFQAPPEE178 pKa = 4.5 VYY180 pKa = 10.36 VSPFCASSFTQVPDD194 pKa = 3.38 EE195 pKa = 4.13 LQRR198 pKa = 11.84 WASANVMDD206 pKa = 5.59 AAARR210 pKa = 11.84 PWRR213 pKa = 11.84 PSSIIVEE220 pKa = 4.3 GDD222 pKa = 3.0 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.09 VYY251 pKa = 10.85 SNSAWYY257 pKa = 10.31 NVIDD261 pKa = 5.38 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.46 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.26 YY287 pKa = 10.63 GKK289 pKa = 9.49 PIQIKK294 pKa = 10.42 GGIPTIFLCNPGPTSSYY311 pKa = 11.06 KK312 pKa = 10.58 EE313 pKa = 3.96 YY314 pKa = 10.91 LDD316 pKa = 3.48 EE317 pKa = 5.94 DD318 pKa = 4.11 KK319 pKa = 11.33 NISLKK324 pKa = 10.7 AWALKK329 pKa = 10.05 NAAFCTLTQPLYY341 pKa = 11.08 SSADD345 pKa = 3.35 QSSTPNRR352 pKa = 11.84 EE353 pKa = 4.14 EE354 pKa = 4.29 EE355 pKa = 4.11 SHH357 pKa = 6.53 SAQKK361 pKa = 10.65 DD362 pKa = 3.28
Molecular weight: 40.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.854
IPC2_protein 7.102
IPC_protein 7.059
Toseland 6.883
ProMoST 7.497
Dawson 7.717
Bjellqvist 7.98
Wikipedia 7.644
Rodwell 7.732
Grimsley 6.971
Solomon 7.775
Lehninger 7.79
Nozaki 8.229
DTASelect 7.878
Thurlkill 7.922
EMBOSS 7.966
Sillero 8.214
Patrickios 4.113
IPC_peptide 7.775
IPC2_peptide 7.263
IPC2.peptide.svr19 7.242
Protein with the highest isoelectric point:
>tr|O56816|O56816_9GEMI Replication-associated protein OS=Chayote yellow mosaic virus OX=222450 GN=C1 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.94 IIISTPVSKK16 pKa = 10.56 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.03 SPYY27 pKa = 7.84 TNRR30 pKa = 11.84 VAVPIVQGTNKK41 pKa = 9.27 RR42 pKa = 11.84 RR43 pKa = 11.84 SWTYY47 pKa = 8.86 RR48 pKa = 11.84 PSYY51 pKa = 9.78 RR52 pKa = 11.84 KK53 pKa = 8.69 PRR55 pKa = 11.84 MYY57 pKa = 10.91 RR58 pKa = 11.84 MYY60 pKa = 10.44 RR61 pKa = 11.84 SPDD64 pKa = 3.21 VPRR67 pKa = 11.84 GCEE70 pKa = 4.4 GPCKK74 pKa = 9.97 IQSFDD79 pKa = 3.31 QRR81 pKa = 11.84 DD82 pKa = 4.22 SVVHH86 pKa = 5.94 TGNVRR91 pKa = 11.84 CLSDD95 pKa = 3.23 VTRR98 pKa = 11.84 GGGLTHH104 pKa = 6.43 RR105 pKa = 11.84 TGKK108 pKa = 10.03 RR109 pKa = 11.84 FCIKK113 pKa = 10.13 SVYY116 pKa = 10.35 VLGKK120 pKa = 9.39 VWMDD124 pKa = 3.42 EE125 pKa = 3.95 NVKK128 pKa = 10.46 KK129 pKa = 10.93 SNHH132 pKa = 5.55 TNTCLFWLVRR142 pKa = 11.84 DD143 pKa = 3.72 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 10.66 GSSPQDD153 pKa = 3.22 FGQVFNMFDD162 pKa = 3.99 NEE164 pKa = 4.12 PSTATVMNDD173 pKa = 2.37 KK174 pKa = 10.42 RR175 pKa = 11.84 DD176 pKa = 3.49 RR177 pKa = 11.84 YY178 pKa = 9.22 QVLRR182 pKa = 11.84 RR183 pKa = 11.84 FQVTVTGGPSGCKK196 pKa = 8.84 EE197 pKa = 3.5 AAIVKK202 pKa = 10.02 RR203 pKa = 11.84 FFRR206 pKa = 11.84 LNHH209 pKa = 5.74 HH210 pKa = 5.45 VTYY213 pKa = 10.77 NHH215 pKa = 6.09 QEE217 pKa = 3.49 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 10.67 ASLKK247 pKa = 8.7 IRR249 pKa = 11.84 MYY251 pKa = 10.72 FYY253 pKa = 11.16 DD254 pKa = 3.65 SVINN258 pKa = 3.86
Molecular weight: 29.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.56
IPC_protein 9.94
Toseland 10.058
ProMoST 9.853
Dawson 10.306
Bjellqvist 10.043
Wikipedia 10.511
Rodwell 10.57
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 10.131
DTASelect 10.028
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.233
Patrickios 9.882
IPC_peptide 10.335
IPC2_peptide 9.092
IPC2.peptide.svr19 8.486
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
974
85
362
194.8
22.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.468 ± 1.218
2.259 ± 0.356
5.236 ± 0.342
4.312 ± 0.646
4.517 ± 0.608
4.517 ± 0.519
3.696 ± 0.626
5.647 ± 1.098
5.647 ± 1.068
7.084 ± 1.135
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.745 ± 0.557
5.133 ± 0.842
6.057 ± 0.899
4.62 ± 0.537
7.392 ± 1.537
9.343 ± 1.891
5.236 ± 0.848
6.057 ± 1.455
1.437 ± 0.225
3.593 ± 0.94
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here