Vibrio sp. 10N.286.49.B3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; unclassified Vibrio

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N7DBZ1|A0A2N7DBZ1_9VIBR 2-dehydro-3-deoxyphosphooctonate aldolase OS=Vibrio sp. 10N.286.49.B3 OX=1880855 GN=kdsA PE=3 SV=1
MM1 pKa = 7.78EE2 pKa = 4.55KK3 pKa = 9.63TFKK6 pKa = 10.85LSLVFSAILLAGCGDD21 pKa = 3.88DD22 pKa = 4.38TEE24 pKa = 5.49SSGAPTTPVYY34 pKa = 10.98EE35 pKa = 4.83DD36 pKa = 5.2FIQEE40 pKa = 4.18SLEE43 pKa = 4.06QPTTIQFTLSGQNAAVPLPSFALLDD68 pKa = 3.91TSDD71 pKa = 3.8GTLGLPTGGDD81 pKa = 3.62DD82 pKa = 4.74ALSNPIAAMNTADD95 pKa = 4.06GFSTTMPISLTFEE108 pKa = 4.31GGDD111 pKa = 3.36FGEE114 pKa = 4.54TQNILTSGIYY124 pKa = 9.79VVEE127 pKa = 4.46LSDD130 pKa = 4.42GLTGSPTPQAILSAPTDD147 pKa = 4.05FVTLAQGSNLTIIFNEE163 pKa = 3.86PLNPKK168 pKa = 9.78SNYY171 pKa = 8.95IFAVTNEE178 pKa = 4.05VLDD181 pKa = 3.88SDD183 pKa = 4.48GEE185 pKa = 4.37AVGMSSSYY193 pKa = 11.04AAAKK197 pKa = 10.36SEE199 pKa = 3.93EE200 pKa = 4.21KK201 pKa = 10.26IYY203 pKa = 10.99TEE205 pKa = 4.14GSLAQVQQVTQGVEE219 pKa = 3.78ALFAATGVDD228 pKa = 3.69KK229 pKa = 10.25EE230 pKa = 4.57TIIYY234 pKa = 9.61SSWFITQSVGDD245 pKa = 4.02SLYY248 pKa = 9.58ATKK251 pKa = 10.71GATATGLASGGLNNVWALDD270 pKa = 3.72SQANPNNVDD279 pKa = 3.14LTYY282 pKa = 10.62AYY284 pKa = 9.72IMSFEE289 pKa = 4.11EE290 pKa = 4.0TATFNDD296 pKa = 3.92ALDD299 pKa = 4.09NDD301 pKa = 4.59SDD303 pKa = 4.0FDD305 pKa = 4.94DD306 pKa = 5.71YY307 pKa = 11.44IGSDD311 pKa = 2.99AKK313 pKa = 11.1AGIKK317 pKa = 10.0ALYY320 pKa = 7.78EE321 pKa = 4.12ASDD324 pKa = 3.92AEE326 pKa = 4.64VNVTQGTVNLPHH338 pKa = 7.33YY339 pKa = 10.2LEE341 pKa = 4.49QDD343 pKa = 3.35LATWNSQPFQSAMPSLAKK361 pKa = 10.35ISAALSDD368 pKa = 4.35EE369 pKa = 4.25NEE371 pKa = 4.11QAHH374 pKa = 6.12VAGQLMAAGITPSLLATSPEE394 pKa = 4.09EE395 pKa = 3.71QLKK398 pKa = 10.89LVGQSLTLSDD408 pKa = 4.58GSALDD413 pKa = 3.56SEE415 pKa = 5.09RR416 pKa = 11.84VITQYY421 pKa = 11.55SPVPQVKK428 pKa = 10.16SLEE431 pKa = 4.04AVEE434 pKa = 5.95FILFTPTNSTATEE447 pKa = 3.66IAIYY451 pKa = 9.12QHH453 pKa = 7.18GITSAKK459 pKa = 8.62EE460 pKa = 3.35NAYY463 pKa = 10.53AFAFNQAQQGIAVLAIDD480 pKa = 5.02LPLHH484 pKa = 5.61GTRR487 pKa = 11.84SLDD490 pKa = 3.32EE491 pKa = 4.73DD492 pKa = 3.73RR493 pKa = 11.84SANVDD498 pKa = 3.23TLAYY502 pKa = 10.82LNLTYY507 pKa = 10.9LPVARR512 pKa = 11.84DD513 pKa = 3.62NLRR516 pKa = 11.84QSALDD521 pKa = 3.63VMGLRR526 pKa = 11.84AALAVSSQAGLLTTTPLSAINPVTSSHH553 pKa = 7.38FIGHH557 pKa = 5.77SLGGIVGVSAVAAANEE573 pKa = 4.33SLGSDD578 pKa = 3.54TADD581 pKa = 2.97ALYY584 pKa = 10.5NFSSMSIQNSGGQIANLLLGSTNFGPLIMHH614 pKa = 7.18NIALSLSIGYY624 pKa = 9.91DD625 pKa = 3.09SYY627 pKa = 11.81VAEE630 pKa = 4.43NCTDD634 pKa = 3.68VIEE637 pKa = 4.61QICFAQFSAQATEE650 pKa = 4.09EE651 pKa = 4.12QQAVMSAGFQQFSYY665 pKa = 10.81AAQTVLDD672 pKa = 4.65TIDD675 pKa = 3.85PFTNASHH682 pKa = 6.8LVDD685 pKa = 4.0NATLIPVLMSQVADD699 pKa = 4.34DD700 pKa = 4.02DD701 pKa = 4.45TVPNSVDD708 pKa = 3.8FAPFAGTEE716 pKa = 3.92PLAAQLSLDD725 pKa = 3.85EE726 pKa = 4.52TSTPGTTGGFVKK738 pKa = 9.94FTDD741 pKa = 3.55SAAGHH746 pKa = 5.37STFVAPKK753 pKa = 9.84SDD755 pKa = 3.27LSDD758 pKa = 3.19IVTHH762 pKa = 6.61ATMQSQQTQFIAPTTTPP779 pKa = 3.18

Molecular weight: 81.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N7DCD8|A0A2N7DCD8_9VIBR Cyclic pyranopterin monophosphate synthase OS=Vibrio sp. 10N.286.49.B3 OX=1880855 GN=moaC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.99RR4 pKa = 11.84TFQPTVLKK12 pKa = 10.46RR13 pKa = 11.84KK14 pKa = 7.65RR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.44NGRR29 pKa = 11.84KK30 pKa = 9.39VINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.22GRR40 pKa = 11.84ARR42 pKa = 11.84LSKK45 pKa = 10.83

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3192

0

3192

1073679

35

3119

336.4

37.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.321 ± 0.047

1.016 ± 0.014

5.694 ± 0.039

6.262 ± 0.036

4.136 ± 0.03

6.691 ± 0.043

2.29 ± 0.022

6.787 ± 0.033

5.322 ± 0.039

10.299 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.025

4.432 ± 0.032

3.777 ± 0.025

4.662 ± 0.036

4.325 ± 0.038

6.785 ± 0.037

5.458 ± 0.032

6.799 ± 0.035

1.211 ± 0.017

3.058 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski