Maize striate mosaic virus
Average proteome isoelectric point is 7.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286QV14|A0A286QV14_9GEMI Replication-associated protein OS=Maize striate mosaic virus OX=2025388 PE=3 SV=1
MM1 pKa = 7.24 SHH3 pKa = 5.59 TSFRR7 pKa = 11.84 FRR9 pKa = 11.84 AKK11 pKa = 10.45 NVFLTYY17 pKa = 9.25 PRR19 pKa = 11.84 CPIGPEE25 pKa = 3.94 FLCDD29 pKa = 3.78 HH30 pKa = 7.2 LWNLVTPYY38 pKa = 10.93 DD39 pKa = 3.74 PLYY42 pKa = 10.82 VHH44 pKa = 6.93 VAQEE48 pKa = 3.85 NHH50 pKa = 6.79 KK51 pKa = 11.0 DD52 pKa = 3.66 GGLHH56 pKa = 5.08 SHH58 pKa = 6.28 VLIQTRR64 pKa = 11.84 IEE66 pKa = 4.04 ISTFDD71 pKa = 3.64 PTYY74 pKa = 10.78 FDD76 pKa = 3.58 YY77 pKa = 10.96 TGTSIPGAVVFHH89 pKa = 6.59 PNIQACRR96 pKa = 11.84 NVRR99 pKa = 11.84 DD100 pKa = 3.51 CLAYY104 pKa = 9.62 IRR106 pKa = 11.84 KK107 pKa = 8.28 NTINEE112 pKa = 3.86 VSKK115 pKa = 11.12 GAFKK119 pKa = 10.88 CSGAGRR125 pKa = 11.84 PKK127 pKa = 10.47 KK128 pKa = 9.99 QDD130 pKa = 3.29 SAPSRR135 pKa = 11.84 DD136 pKa = 3.25 AKK138 pKa = 9.94 MCQIMSSATSRR149 pKa = 11.84 SDD151 pKa = 3.35 YY152 pKa = 11.09 LSMVRR157 pKa = 11.84 GAFPFEE163 pKa = 4.23 WATKK167 pKa = 9.6 LAQFEE172 pKa = 4.47 YY173 pKa = 10.32 SASKK177 pKa = 10.5 LFPDD181 pKa = 4.87 APTTNAIPSNIDD193 pKa = 3.2 LTCHH197 pKa = 5.53 EE198 pKa = 5.77 NIMPWLRR205 pKa = 11.84 DD206 pKa = 3.36 DD207 pKa = 5.24 LYY209 pKa = 10.42 TVSEE213 pKa = 4.46 FSYY216 pKa = 9.75 FLCTGSLDD224 pKa = 3.88 PKK226 pKa = 11.42 GDD228 pKa = 3.92 LTWMADD234 pKa = 3.14 HH235 pKa = 6.72 TRR237 pKa = 11.84 NEE239 pKa = 4.23 GKK241 pKa = 10.34 EE242 pKa = 4.03 DD243 pKa = 3.48 VPEE246 pKa = 4.39 ASTYY250 pKa = 10.43 AEE252 pKa = 3.71 EE253 pKa = 4.08 RR254 pKa = 11.84 GRR256 pKa = 11.84 ARR258 pKa = 11.84 QHH260 pKa = 4.96 GQEE263 pKa = 4.18 ASGHH267 pKa = 4.62 TTTSNTRR274 pKa = 11.84 WTTSPGAKK282 pKa = 8.8 RR283 pKa = 11.84 PRR285 pKa = 11.84 MSSS288 pKa = 2.83
Molecular weight: 32.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.768
IPC2_protein 6.854
IPC_protein 6.912
Toseland 6.81
ProMoST 7.322
Dawson 7.512
Bjellqvist 7.644
Wikipedia 7.454
Rodwell 7.512
Grimsley 6.912
Solomon 7.571
Lehninger 7.585
Nozaki 7.951
DTASelect 7.732
Thurlkill 7.761
EMBOSS 7.805
Sillero 7.995
Patrickios 4.088
IPC_peptide 7.571
IPC2_peptide 7.366
IPC2.peptide.svr19 7.161
Protein with the highest isoelectric point:
>tr|A0A286QV10|A0A286QV10_9GEMI Replication-associated protein OS=Maize striate mosaic virus OX=2025388 PE=3 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 PMTGKK7 pKa = 10.3 RR8 pKa = 11.84 KK9 pKa = 8.45 RR10 pKa = 11.84 TAGSSSVNRR19 pKa = 11.84 RR20 pKa = 11.84 KK21 pKa = 9.9 SRR23 pKa = 11.84 RR24 pKa = 11.84 VLPRR28 pKa = 11.84 TVAPYY33 pKa = 9.98 SQLPSRR39 pKa = 11.84 LPSLQVQTFASQGSAVVEE57 pKa = 4.33 VKK59 pKa = 10.75 KK60 pKa = 10.94 GGNCLMVTSYY70 pKa = 11.4 SRR72 pKa = 11.84 GSDD75 pKa = 2.84 EE76 pKa = 4.53 SQRR79 pKa = 11.84 HH80 pKa = 4.42 TNEE83 pKa = 3.16 TMTYY87 pKa = 10.56 KK88 pKa = 10.15 MSLDD92 pKa = 3.23 HH93 pKa = 8.04 VMVLRR98 pKa = 11.84 AEE100 pKa = 4.02 LCKK103 pKa = 10.82 YY104 pKa = 9.98 SFKK107 pKa = 10.29 ATHH110 pKa = 6.59 CGWVVYY116 pKa = 9.41 DD117 pKa = 5.21 AKK119 pKa = 10.29 PTGNQVTCKK128 pKa = 10.05 TIFGYY133 pKa = 9.92 PDD135 pKa = 3.55 GLVDD139 pKa = 6.11 YY140 pKa = 7.46 PTTWKK145 pKa = 10.15 VARR148 pKa = 11.84 DD149 pKa = 3.42 VAHH152 pKa = 6.56 RR153 pKa = 11.84 FVVKK157 pKa = 10.51 KK158 pKa = 9.24 RR159 pKa = 11.84 WTYY162 pKa = 10.6 RR163 pKa = 11.84 MEE165 pKa = 4.89 SNGSNSSKK173 pKa = 10.51 DD174 pKa = 3.19 WSNGTGIPPCNRR186 pKa = 11.84 SVYY189 pKa = 10.15 VKK191 pKa = 10.57 QFVSKK196 pKa = 10.29 LNCRR200 pKa = 11.84 TEE202 pKa = 4.0 WKK204 pKa = 8.44 NTTGGDD210 pKa = 3.41 YY211 pKa = 11.46 GDD213 pKa = 3.82 IKK215 pKa = 11.12 GGALYY220 pKa = 10.58 VVLAAGQGMEE230 pKa = 3.86 HH231 pKa = 6.38 MAYY234 pKa = 8.4 GTTRR238 pKa = 11.84 MYY240 pKa = 10.63 FKK242 pKa = 10.96 SIGNQQ247 pKa = 3.0
Molecular weight: 27.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.244
IPC2_protein 9.487
IPC_protein 9.575
Toseland 10.043
ProMoST 9.765
Dawson 10.262
Bjellqvist 9.97
Wikipedia 10.452
Rodwell 10.657
Grimsley 10.35
Solomon 10.292
Lehninger 10.248
Nozaki 10.087
DTASelect 9.955
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.189
Patrickios 10.116
IPC_peptide 10.292
IPC2_peptide 8.843
IPC2.peptide.svr19 8.39
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
998
120
343
249.5
28.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.014 ± 0.697
2.605 ± 0.184
5.01 ± 0.598
4.208 ± 0.417
4.81 ± 0.738
6.613 ± 0.8
2.806 ± 0.605
4.008 ± 0.985
5.311 ± 0.732
6.112 ± 0.72
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.705 ± 0.398
4.008 ± 0.489
6.313 ± 0.742
3.106 ± 0.183
6.613 ± 0.388
8.116 ± 0.579
7.615 ± 0.56
6.613 ± 1.315
2.004 ± 0.136
4.409 ± 0.397
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here