Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Circumdati; Aspergillus oryzae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12057 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2U4E5|Q2U4E5_ASPOR Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000375 PE=3 SV=1
MM1 pKa = 7.69TYY3 pKa = 10.41YY4 pKa = 10.76SQWFCMAVAEE14 pKa = 4.55DD15 pKa = 3.36RR16 pKa = 11.84TYY18 pKa = 10.71YY19 pKa = 11.04ARR21 pKa = 11.84DD22 pKa = 3.16GGAAFYY28 pKa = 10.47RR29 pKa = 11.84AIYY32 pKa = 9.13TGGDD36 pKa = 3.08AYY38 pKa = 11.38LDD40 pKa = 3.73TEE42 pKa = 4.38EE43 pKa = 4.57QANTNAFHH51 pKa = 6.16MSVYY55 pKa = 8.92RR56 pKa = 11.84AKK58 pKa = 10.42MLDD61 pKa = 3.07KK62 pKa = 10.72ALNILKK68 pKa = 10.32SGMSKK73 pKa = 10.27FVSVLLVNEE82 pKa = 4.03SHH84 pKa = 7.03YY85 pKa = 11.5DD86 pKa = 3.42PDD88 pKa = 4.67LMGEE92 pKa = 4.5LPYY95 pKa = 9.75LTCCKK100 pKa = 10.22VDD102 pKa = 4.2DD103 pKa = 5.14EE104 pKa = 4.37EE105 pKa = 5.31LPWVTWPNDD114 pKa = 3.22SDD116 pKa = 3.63QDD118 pKa = 3.28EE119 pKa = 4.56DD120 pKa = 4.47AEE122 pKa = 4.21IDD124 pKa = 3.29ITEE127 pKa = 4.46PEE129 pKa = 4.37STTGEE134 pKa = 4.22VTSADD139 pKa = 3.4TSATFATDD147 pKa = 3.52TLPVDD152 pKa = 3.51TDD154 pKa = 3.22ITDD157 pKa = 3.44VEE159 pKa = 4.73SIEE162 pKa = 4.64TEE164 pKa = 4.17DD165 pKa = 3.57EE166 pKa = 4.07NKK168 pKa = 10.17NASVVDD174 pKa = 3.79EE175 pKa = 4.52AEE177 pKa = 3.93EE178 pKa = 4.13RR179 pKa = 11.84TTAEE183 pKa = 3.76

Molecular weight: 20.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2UTT8|Q2UTT8_ASPOR Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000596 PE=3 SV=1
MM1 pKa = 7.51SPLATLRR8 pKa = 11.84RR9 pKa = 11.84SRR11 pKa = 11.84PRR13 pKa = 11.84CRR15 pKa = 11.84VNMCQRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 9.3RR25 pKa = 11.84KK26 pKa = 7.93RR27 pKa = 11.84NKK29 pKa = 7.86RR30 pKa = 11.84QRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84SRR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 7.97PRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84MKK44 pKa = 9.81RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84MKK49 pKa = 10.35RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84LKK54 pKa = 11.11AMSLSLKK61 pKa = 9.99RR62 pKa = 11.84STRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84ARR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84TRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84ARR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84TRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84KK85 pKa = 4.6TTKK88 pKa = 9.11KK89 pKa = 9.27RR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84KK94 pKa = 8.9PIMMMRR100 pKa = 11.84MQVFSRR106 pKa = 11.84KK107 pKa = 9.37SSPTSTLSAKK117 pKa = 10.45LNEE120 pKa = 4.24KK121 pKa = 10.37VVVSKK126 pKa = 11.14LGLGAGITYY135 pKa = 8.25ATVCAMGPEE144 pKa = 4.1

Molecular weight: 17.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12055

2

12057

5416970

69

6885

449.3

49.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.251 ± 0.017

1.309 ± 0.009

5.575 ± 0.016

6.069 ± 0.021

3.854 ± 0.014

6.878 ± 0.021

2.419 ± 0.009

5.168 ± 0.016

4.628 ± 0.017

9.228 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.008

3.733 ± 0.012

5.813 ± 0.023

3.987 ± 0.015

5.947 ± 0.019

8.198 ± 0.024

5.954 ± 0.015

6.318 ± 0.016

1.533 ± 0.008

2.952 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski