Amino acid dipepetide frequency for Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.126AlaAla: 8.126 ± 0.054	1.107AlaCys: 1.107 ± 0.016	4.066AlaAsp: 4.066 ± 0.031	4.865AlaGlu: 4.865 ± 0.038	3.207AlaPhe: 3.207 ± 0.024	5.635AlaGly: 5.635 ± 0.039	1.754AlaHis: 1.754 ± 0.022	4.411AlaIle: 4.411 ± 0.036	3.747AlaLys: 3.747 ± 0.029	7.796AlaLeu: 7.796 ± 0.043	1.993AlaMet: 1.993 ± 0.02	2.888AlaAsn: 2.888 ± 0.022	4.319AlaPro: 4.319 ± 0.04	3.221AlaGln: 3.221 ± 0.03	4.615AlaArg: 4.615 ± 0.034	6.832AlaSer: 6.832 ± 0.039	5.077AlaThr: 5.077 ± 0.03	5.401AlaVal: 5.401 ± 0.032	1.187AlaTrp: 1.187 ± 0.016	2.261AlaTyr: 2.261 ± 0.019	0.0AlaXaa: 0.0 ± 0.0
Cys	1.009CysAla: 1.009 ± 0.014	0.263CysCys: 0.263 ± 0.008	0.683CysAsp: 0.683 ± 0.012	0.618CysGlu: 0.618 ± 0.012	0.588CysPhe: 0.588 ± 0.011	0.963CysGly: 0.963 ± 0.016	0.354CysHis: 0.354 ± 0.009	0.771CysIle: 0.771 ± 0.013	0.508CysLys: 0.508 ± 0.011	1.399CysLeu: 1.399 ± 0.018	0.302CysMet: 0.302 ± 0.007	0.459CysAsn: 0.459 ± 0.008	0.697CysPro: 0.697 ± 0.014	0.488CysGln: 0.488 ± 0.01	0.789CysArg: 0.789 ± 0.012	0.999CysSer: 0.999 ± 0.016	0.731CysThr: 0.731 ± 0.013	0.864CysVal: 0.864 ± 0.014	0.213CysTrp: 0.213 ± 0.006	0.39CysTyr: 0.39 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.394AspAla: 4.394 ± 0.028	0.626AspCys: 0.626 ± 0.012	3.731AspAsp: 3.731 ± 0.036	4.107AspGlu: 4.107 ± 0.035	2.157AspPhe: 2.157 ± 0.024	3.946AspGly: 3.946 ± 0.031	1.269AspHis: 1.269 ± 0.015	3.257AspIle: 3.257 ± 0.027	2.276AspLys: 2.276 ± 0.022	5.154AspLeu: 5.154 ± 0.032	1.259AspMet: 1.259 ± 0.017	1.938AspAsn: 1.938 ± 0.019	3.329AspPro: 3.329 ± 0.026	1.927AspGln: 1.927 ± 0.02	3.031AspArg: 3.031 ± 0.028	4.07AspSer: 4.07 ± 0.033	3.025AspThr: 3.025 ± 0.026	3.672AspVal: 3.672 ± 0.027	0.884AspTrp: 0.884 ± 0.013	1.693AspTyr: 1.693 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	5.116GluAla: 5.116 ± 0.034	0.663GluCys: 0.663 ± 0.011	3.941GluAsp: 3.941 ± 0.034	5.197GluGlu: 5.197 ± 0.047	1.936GluPhe: 1.936 ± 0.018	3.691GluGly: 3.691 ± 0.026	1.387GluHis: 1.387 ± 0.017	3.09GluIle: 3.09 ± 0.026	3.63GluLys: 3.63 ± 0.028	5.242GluLeu: 5.242 ± 0.042	1.399GluMet: 1.399 ± 0.016	2.357GluAsn: 2.357 ± 0.021	2.702GluPro: 2.702 ± 0.028	2.471GluGln: 2.471 ± 0.025	3.825GluArg: 3.825 ± 0.034	4.307GluSer: 4.307 ± 0.036	3.525GluThr: 3.525 ± 0.03	3.564GluVal: 3.564 ± 0.027	0.894GluTrp: 0.894 ± 0.013	1.757GluTyr: 1.757 ± 0.016	0.0GluXaa: 0.0 ± 0.0
Phe	3.041PheAla: 3.041 ± 0.025	0.606PheCys: 0.606 ± 0.011	2.263PheAsp: 2.263 ± 0.02	2.097PheGlu: 2.097 ± 0.021	1.783PhePhe: 1.783 ± 0.025	2.907PheGly: 2.907 ± 0.024	0.992PheHis: 0.992 ± 0.014	1.981PheIle: 1.981 ± 0.023	1.478PheLys: 1.478 ± 0.018	3.781PheLeu: 3.781 ± 0.032	0.831PheMet: 0.831 ± 0.011	1.512PheAsn: 1.512 ± 0.02	2.059PhePro: 2.059 ± 0.019	1.464PheGln: 1.464 ± 0.017	2.017PheArg: 2.017 ± 0.02	3.092PheSer: 3.092 ± 0.023	2.241PheThr: 2.241 ± 0.021	2.483PheVal: 2.483 ± 0.023	0.68PheTrp: 0.68 ± 0.013	1.227PheTyr: 1.227 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	5.204GlyAla: 5.204 ± 0.035	0.965GlyCys: 0.965 ± 0.016	3.603GlyAsp: 3.603 ± 0.028	3.579GlyGlu: 3.579 ± 0.028	2.938GlyPhe: 2.938 ± 0.025	5.402GlyGly: 5.402 ± 0.051	1.712GlyHis: 1.712 ± 0.02	3.8GlyIle: 3.8 ± 0.03	3.358GlyLys: 3.358 ± 0.028	6.375GlyLeu: 6.375 ± 0.039	1.591GlyMet: 1.591 ± 0.019	2.583GlyAsn: 2.583 ± 0.023	3.271GlyPro: 3.271 ± 0.031	2.591GlyGln: 2.591 ± 0.025	3.971GlyArg: 3.971 ± 0.034	5.715GlySer: 5.715 ± 0.047	4.002GlyThr: 4.002 ± 0.031	4.614GlyVal: 4.614 ± 0.036	1.23GlyTrp: 1.23 ± 0.017	2.276GlyTyr: 2.276 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	1.797HisAla: 1.797 ± 0.02	0.359HisCys: 0.359 ± 0.009	1.333HisAsp: 1.333 ± 0.015	1.312HisGlu: 1.312 ± 0.016	0.955HisPhe: 0.955 ± 0.014	1.743HisGly: 1.743 ± 0.021	0.834HisHis: 0.834 ± 0.017	1.308HisIle: 1.308 ± 0.014	0.89HisLys: 0.89 ± 0.013	2.326HisLeu: 2.326 ± 0.021	0.518HisMet: 0.518 ± 0.009	0.877HisAsn: 0.877 ± 0.013	1.67HisPro: 1.67 ± 0.018	0.939HisGln: 0.939 ± 0.014	1.557HisArg: 1.557 ± 0.02	1.886HisSer: 1.886 ± 0.022	1.312HisThr: 1.312 ± 0.019	1.456HisVal: 1.456 ± 0.017	0.385HisTrp: 0.385 ± 0.008	0.733HisTyr: 0.733 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.337IleAla: 4.337 ± 0.03	0.842IleCys: 0.842 ± 0.014	2.912IleAsp: 2.912 ± 0.027	2.917IleGlu: 2.917 ± 0.022	2.155IlePhe: 2.155 ± 0.023	3.45IleGly: 3.45 ± 0.03	1.317IleHis: 1.317 ± 0.016	2.732IleIle: 2.732 ± 0.029	2.101IleLys: 2.101 ± 0.022	4.943IleLeu: 4.943 ± 0.032	1.085IleMet: 1.085 ± 0.015	1.876IleAsn: 1.876 ± 0.017	3.222IlePro: 3.222 ± 0.027	2.038IleGln: 2.038 ± 0.022	2.884IleArg: 2.884 ± 0.024	4.079IleSer: 4.079 ± 0.029	2.988IleThr: 2.988 ± 0.027	3.411IleVal: 3.411 ± 0.029	0.784IleTrp: 0.784 ± 0.013	1.61IleTyr: 1.61 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.961LysAla: 3.961 ± 0.033	0.513LysCys: 0.513 ± 0.011	2.764LysAsp: 2.764 ± 0.023	3.285LysGlu: 3.285 ± 0.026	1.415LysPhe: 1.415 ± 0.017	2.972LysGly: 2.972 ± 0.028	1.102LysHis: 1.102 ± 0.015	2.196LysIle: 2.196 ± 0.021	2.911LysLys: 2.911 ± 0.038	4.007LysLeu: 4.007 ± 0.032	0.917LysMet: 0.917 ± 0.013	1.741LysAsn: 1.741 ± 0.021	2.566LysPro: 2.566 ± 0.029	1.825LysGln: 1.825 ± 0.019	3.127LysArg: 3.127 ± 0.028	3.356LysSer: 3.356 ± 0.026	2.681LysThr: 2.681 ± 0.024	2.845LysVal: 2.845 ± 0.029	0.673LysTrp: 0.673 ± 0.01	1.426LysTyr: 1.426 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	7.78LeuAla: 7.78 ± 0.044	1.334LeuCys: 1.334 ± 0.018	5.23LeuAsp: 5.23 ± 0.03	5.576LeuGlu: 5.576 ± 0.047	3.597LeuPhe: 3.597 ± 0.028	6.241LeuGly: 6.241 ± 0.043	2.344LeuHis: 2.344 ± 0.023	4.296LeuIle: 4.296 ± 0.03	4.129LeuLys: 4.129 ± 0.028	8.916LeuLeu: 8.916 ± 0.057	1.921LeuMet: 1.921 ± 0.021	3.346LeuAsn: 3.346 ± 0.025	5.454LeuPro: 5.454 ± 0.036	3.941LeuGln: 3.941 ± 0.028	5.79LeuArg: 5.79 ± 0.042	7.689LeuSer: 7.689 ± 0.04	5.061LeuThr: 5.061 ± 0.032	5.721LeuVal: 5.721 ± 0.036	1.306LeuTrp: 1.306 ± 0.016	2.603LeuTyr: 2.603 ± 0.022	0.0LeuXaa: 0.0 ± 0.0
Met	2.141MetAla: 2.141 ± 0.019	0.278MetCys: 0.278 ± 0.007	1.238MetAsp: 1.238 ± 0.016	1.296MetGlu: 1.296 ± 0.016	0.777MetPhe: 0.777 ± 0.012	1.512MetGly: 1.512 ± 0.021	0.487MetHis: 0.487 ± 0.01	1.08MetIle: 1.08 ± 0.016	1.03MetLys: 1.03 ± 0.013	1.917MetLeu: 1.917 ± 0.021	0.559MetMet: 0.559 ± 0.013	0.837MetAsn: 0.837 ± 0.013	1.18MetPro: 1.18 ± 0.014	0.867MetGln: 0.867 ± 0.013	1.242MetArg: 1.242 ± 0.015	1.867MetSer: 1.867 ± 0.017	1.322MetThr: 1.322 ± 0.015	1.403MetVal: 1.403 ± 0.016	0.279MetTrp: 0.279 ± 0.007	0.561MetTyr: 0.561 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	3.107AsnAla: 3.107 ± 0.023	0.49AsnCys: 0.49 ± 0.009	1.967AsnAsp: 1.967 ± 0.02	2.07AsnGlu: 2.07 ± 0.021	1.38AsnPhe: 1.38 ± 0.018	2.968AsnGly: 2.968 ± 0.029	0.896AsnHis: 0.896 ± 0.012	2.227AsnIle: 2.227 ± 0.019	1.532AsnLys: 1.532 ± 0.019	3.358AsnLeu: 3.358 ± 0.026	0.874AsnMet: 0.874 ± 0.013	1.483AsnAsn: 1.483 ± 0.017	2.501AsnPro: 2.501 ± 0.021	1.355AsnGln: 1.355 ± 0.02	1.957AsnArg: 1.957 ± 0.021	2.737AsnSer: 2.737 ± 0.023	2.237AsnThr: 2.237 ± 0.022	2.461AsnVal: 2.461 ± 0.022	0.598AsnTrp: 0.598 ± 0.011	1.13AsnTyr: 1.13 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	4.648ProAla: 4.648 ± 0.037	0.569ProCys: 0.569 ± 0.011	3.152ProAsp: 3.152 ± 0.026	3.85ProGlu: 3.85 ± 0.033	2.142ProPhe: 2.142 ± 0.018	3.843ProGly: 3.843 ± 0.032	1.265ProHis: 1.265 ± 0.017	2.583ProIle: 2.583 ± 0.025	2.509ProLys: 2.509 ± 0.026	4.756ProLeu: 4.756 ± 0.034	1.055ProMet: 1.055 ± 0.016	2.185ProAsn: 2.185 ± 0.02	4.334ProPro: 4.334 ± 0.069	2.363ProGln: 2.363 ± 0.029	3.256ProArg: 3.256 ± 0.03	5.771ProSer: 5.771 ± 0.047	3.827ProThr: 3.827 ± 0.037	3.645ProVal: 3.645 ± 0.032	0.802ProTrp: 0.802 ± 0.013	1.576ProTyr: 1.576 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.313GlnAla: 3.313 ± 0.026	0.472GlnCys: 0.472 ± 0.01	2.03GlnAsp: 2.03 ± 0.02	2.463GlnGlu: 2.463 ± 0.024	1.336GlnPhe: 1.336 ± 0.016	2.501GlnGly: 2.501 ± 0.021	1.023GlnHis: 1.023 ± 0.015	1.967GlnIle: 1.967 ± 0.019	1.983GlnLys: 1.983 ± 0.021	3.579GlnLeu: 3.579 ± 0.029	0.893GlnMet: 0.893 ± 0.014	1.582GlnAsn: 1.582 ± 0.017	2.418GlnPro: 2.418 ± 0.032	2.195GlnGln: 2.195 ± 0.042	2.543GlnArg: 2.543 ± 0.022	3.132GlnSer: 3.132 ± 0.029	2.351GlnThr: 2.351 ± 0.022	2.241GlnVal: 2.241 ± 0.02	0.619GlnTrp: 0.619 ± 0.012	1.223GlnTyr: 1.223 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	4.455ArgAla: 4.455 ± 0.031	0.728ArgCys: 0.728 ± 0.013	3.256ArgAsp: 3.256 ± 0.026	3.726ArgGlu: 3.726 ± 0.031	2.227ArgPhe: 2.227 ± 0.023	3.569ArgGly: 3.569 ± 0.027	1.549ArgHis: 1.549 ± 0.018	2.95ArgIle: 2.95 ± 0.023	3.25ArgLys: 3.25 ± 0.028	5.602ArgLeu: 5.602 ± 0.038	1.292ArgMet: 1.292 ± 0.016	2.246ArgAsn: 2.246 ± 0.021	3.259ArgPro: 3.259 ± 0.03	2.529ArgGln: 2.529 ± 0.027	4.786ArgArg: 4.786 ± 0.042	4.622ArgSer: 4.622 ± 0.038	3.215ArgThr: 3.215 ± 0.025	3.457ArgVal: 3.457 ± 0.027	0.993ArgTrp: 0.993 ± 0.016	1.762ArgTyr: 1.762 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.391SerAla: 6.391 ± 0.035	0.959SerCys: 0.959 ± 0.012	4.193SerAsp: 4.193 ± 0.034	4.21SerGlu: 4.21 ± 0.03	3.172SerPhe: 3.172 ± 0.023	5.611SerGly: 5.611 ± 0.043	1.967SerHis: 1.967 ± 0.021	4.147SerIle: 4.147 ± 0.033	3.604SerLys: 3.604 ± 0.029	7.552SerLeu: 7.552 ± 0.042	1.741SerMet: 1.741 ± 0.02	3.04SerAsn: 3.04 ± 0.022	5.188SerPro: 5.188 ± 0.051	3.286SerGln: 3.286 ± 0.031	4.856SerArg: 4.856 ± 0.037	8.428SerSer: 8.428 ± 0.075	5.448SerThr: 5.448 ± 0.042	4.801SerVal: 4.801 ± 0.031	1.195SerTrp: 1.195 ± 0.015	2.186SerTyr: 2.186 ± 0.02	0.0SerXaa: 0.0 ± 0.0
Thr	5.054ThrAla: 5.054 ± 0.034	0.769ThrCys: 0.769 ± 0.013	2.959ThrAsp: 2.959 ± 0.026	3.211ThrGlu: 3.211 ± 0.028	2.308ThrPhe: 2.308 ± 0.024	4.323ThrGly: 4.323 ± 0.033	1.302ThrHis: 1.302 ± 0.017	3.239ThrIle: 3.239 ± 0.027	2.551ThrLys: 2.551 ± 0.025	5.404ThrLeu: 5.404 ± 0.034	1.224ThrMet: 1.224 ± 0.014	2.15ThrAsn: 2.15 ± 0.021	4.101ThrPro: 4.101 ± 0.041	2.082ThrGln: 2.082 ± 0.021	2.984ThrArg: 2.984 ± 0.025	5.174ThrSer: 5.174 ± 0.04	4.103ThrThr: 4.103 ± 0.036	3.982ThrVal: 3.982 ± 0.03	0.903ThrTrp: 0.903 ± 0.015	1.714ThrTyr: 1.714 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	5.22ValAla: 5.22 ± 0.036	0.903ValCys: 0.903 ± 0.014	3.795ValAsp: 3.795 ± 0.028	3.836ValGlu: 3.836 ± 0.029	2.611ValPhe: 2.611 ± 0.025	4.24ValGly: 4.24 ± 0.03	1.467ValHis: 1.467 ± 0.017	3.211ValIle: 3.211 ± 0.025	2.819ValLys: 2.819 ± 0.025	5.85ValLeu: 5.85 ± 0.033	1.366ValMet: 1.366 ± 0.016	2.353ValAsn: 2.353 ± 0.021	3.617ValPro: 3.617 ± 0.027	2.488ValGln: 2.488 ± 0.023	3.505ValArg: 3.505 ± 0.028	4.944ValSer: 4.944 ± 0.034	3.69ValThr: 3.69 ± 0.029	4.446ValVal: 4.446 ± 0.037	0.922ValTrp: 0.922 ± 0.013	1.899ValTyr: 1.899 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.155TrpAla: 1.155 ± 0.016	0.209TrpCys: 0.209 ± 0.006	0.946TrpAsp: 0.946 ± 0.015	0.874TrpGlu: 0.874 ± 0.014	0.575TrpPhe: 0.575 ± 0.011	1.0TrpGly: 1.0 ± 0.016	0.37TrpHis: 0.37 ± 0.008	0.831TrpIle: 0.831 ± 0.012	0.851TrpLys: 0.851 ± 0.014	1.444TrpLeu: 1.444 ± 0.016	0.395TrpMet: 0.395 ± 0.008	0.68TrpAsn: 0.68 ± 0.012	0.632TrpPro: 0.632 ± 0.012	0.587TrpGln: 0.587 ± 0.011	0.99TrpArg: 0.99 ± 0.015	1.12TrpSer: 1.12 ± 0.015	0.957TrpThr: 0.957 ± 0.014	0.946TrpVal: 0.946 ± 0.013	0.291TrpTrp: 0.291 ± 0.007	0.475TrpTyr: 0.475 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.259TyrAla: 2.259 ± 0.02	0.434TyrCys: 0.434 ± 0.009	1.685TyrAsp: 1.685 ± 0.02	1.603TyrGlu: 1.603 ± 0.018	1.276TyrPhe: 1.276 ± 0.016	2.259TyrGly: 2.259 ± 0.022	0.8TyrHis: 0.8 ± 0.012	1.603TyrIle: 1.603 ± 0.017	1.127TyrLys: 1.127 ± 0.017	2.874TyrLeu: 2.874 ± 0.02	0.657TyrMet: 0.657 ± 0.012	1.192TyrAsn: 1.192 ± 0.015	1.615TyrPro: 1.615 ± 0.019	1.207TyrGln: 1.207 ± 0.014	1.749TyrArg: 1.749 ± 0.02	2.155TyrSer: 2.155 ± 0.022	1.744TyrThr: 1.744 ± 0.017	1.77TyrVal: 1.77 ± 0.019	0.491TyrTrp: 0.491 ± 0.01	1.019TyrTyr: 1.019 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 12057 proteins (5416970 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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