Pseudoalteromonas phage RIO-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Melvirus; Pseudoalteromonas virus RIO1

Average proteome isoelectric point is 5.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JN11|R4JN11_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage RIO-1 OX=1316739 GN=RIO-1_46 PE=4 SV=1
MM1 pKa = 7.69SDD3 pKa = 4.56LYY5 pKa = 11.0QLYY8 pKa = 10.85AFVTDD13 pKa = 4.07KK14 pKa = 11.2SWVDD18 pKa = 4.0FLHH21 pKa = 6.37TSWPDD26 pKa = 2.93APLINGYY33 pKa = 9.59RR34 pKa = 11.84VLVFTNQDD42 pKa = 3.27YY43 pKa = 9.55PRR45 pKa = 11.84LKK47 pKa = 9.82EE48 pKa = 3.77QYY50 pKa = 9.68PDD52 pKa = 3.75FEE54 pKa = 4.69EE55 pKa = 5.89KK56 pKa = 10.63EE57 pKa = 4.23LTTDD61 pKa = 3.15EE62 pKa = 4.83TIAAMEE68 pKa = 4.18SSEE71 pKa = 4.64LGPFVHH77 pKa = 6.82SLAEE81 pKa = 4.05TKK83 pKa = 10.56QILNYY88 pKa = 7.44FTPEE92 pKa = 3.87VNEE95 pKa = 4.27GG96 pKa = 3.21

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JE29|R4JE29_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage RIO-1 OX=1316739 GN=RIO-1_43 PE=4 SV=1
MM1 pKa = 7.46GIEE4 pKa = 4.14VAIGAAIVGSVASVASNLKK23 pKa = 10.45AGSEE27 pKa = 4.12TRR29 pKa = 11.84KK30 pKa = 10.16ASRR33 pKa = 11.84AQARR37 pKa = 11.84IDD39 pKa = 3.05SRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84QRR45 pKa = 11.84EE46 pKa = 3.19QLAQVRR52 pKa = 11.84QGQIAAAQGSQGAATGGTLDD72 pKa = 3.19SSGYY76 pKa = 9.85RR77 pKa = 11.84GSTSSIQSTTSSNIAFTNQIQGLQNYY103 pKa = 9.34SSSRR107 pKa = 11.84LQSANNAQVLGGVVSSIAGAVGSIAMAGTQPTEE140 pKa = 4.02KK141 pKa = 10.3PNTVQQ146 pKa = 4.29

Molecular weight: 14.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13762

40

1607

245.8

27.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.027 ± 0.448

1.046 ± 0.17

6.707 ± 0.205

7.223 ± 0.241

3.64 ± 0.211

7.201 ± 0.346

1.918 ± 0.157

4.992 ± 0.196

6.104 ± 0.33

8.342 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.133

4.353 ± 0.203

3.72 ± 0.205

4.309 ± 0.29

5.072 ± 0.201

7.288 ± 0.321

6.474 ± 0.251

6.685 ± 0.21

1.41 ± 0.151

4.04 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski