Amino acid dipepetide frequency for Pseudoalteromonas phage RIO-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.412AlaAla: 7.412 ± 1.171
0.509AlaCys: 0.509 ± 0.207
4.433AlaAsp: 4.433 ± 0.444
5.014AlaGlu: 5.014 ± 0.674
2.107AlaPhe: 2.107 ± 0.281
5.16AlaGly: 5.16 ± 0.701
1.671AlaHis: 1.671 ± 0.319
3.052AlaIle: 3.052 ± 0.532
4.36AlaLys: 4.36 ± 0.713
5.232AlaLeu: 5.232 ± 0.703
2.035AlaMet: 2.035 ± 0.401
2.979AlaAsn: 2.979 ± 0.434
2.471AlaPro: 2.471 ± 0.444
3.197AlaGln: 3.197 ± 0.495
3.488AlaArg: 3.488 ± 0.58
4.36AlaSer: 4.36 ± 0.558
4.287AlaThr: 4.287 ± 0.573
5.087AlaVal: 5.087 ± 0.529
1.017AlaTrp: 1.017 ± 0.291
2.398AlaTyr: 2.398 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.291CysAla: 0.291 ± 0.142
0.145CysCys: 0.145 ± 0.095
0.363CysAsp: 0.363 ± 0.173
0.799CysGlu: 0.799 ± 0.263
0.436CysPhe: 0.436 ± 0.197
1.308CysGly: 1.308 ± 0.365
0.291CysHis: 0.291 ± 0.164
0.654CysIle: 0.654 ± 0.256
1.09CysLys: 1.09 ± 0.307
1.453CysLeu: 1.453 ± 0.336
0.145CysMet: 0.145 ± 0.105
0.218CysAsn: 0.218 ± 0.121
0.291CysPro: 0.291 ± 0.173
0.363CysGln: 0.363 ± 0.204
0.291CysArg: 0.291 ± 0.135
0.509CysSer: 0.509 ± 0.192
0.509CysThr: 0.509 ± 0.201
0.291CysVal: 0.291 ± 0.147
0.363CysTrp: 0.363 ± 0.165
0.654CysTyr: 0.654 ± 0.202
0.0CysXaa: 0.0 ± 0.0
Asp
5.523AspAla: 5.523 ± 0.679
0.581AspCys: 0.581 ± 0.25
3.997AspAsp: 3.997 ± 0.531
4.796AspGlu: 4.796 ± 0.532
3.27AspPhe: 3.27 ± 0.437
4.433AspGly: 4.433 ± 0.515
0.654AspHis: 0.654 ± 0.239
3.851AspIle: 3.851 ± 0.653
4.142AspLys: 4.142 ± 0.557
5.305AspLeu: 5.305 ± 0.773
1.817AspMet: 1.817 ± 0.365
1.889AspAsn: 1.889 ± 0.393
2.616AspPro: 2.616 ± 0.509
1.671AspGln: 1.671 ± 0.285
3.197AspArg: 3.197 ± 0.49
5.814AspSer: 5.814 ± 0.562
4.215AspThr: 4.215 ± 0.534
5.16AspVal: 5.16 ± 0.511
1.163AspTrp: 1.163 ± 0.313
2.979AspTyr: 2.979 ± 0.425
0.0AspXaa: 0.0 ± 0.0
Glu
4.869GluAla: 4.869 ± 0.732
0.291GluCys: 0.291 ± 0.154
5.087GluAsp: 5.087 ± 0.569
5.232GluGlu: 5.232 ± 0.664
2.471GluPhe: 2.471 ± 0.417
5.45GluGly: 5.45 ± 0.686
1.308GluHis: 1.308 ± 0.281
3.779GluIle: 3.779 ± 0.561
3.779GluLys: 3.779 ± 0.493
7.267GluLeu: 7.267 ± 0.697
1.526GluMet: 1.526 ± 0.295
2.543GluAsn: 2.543 ± 0.369
1.599GluPro: 1.599 ± 0.298
3.779GluGln: 3.779 ± 0.544
3.343GluArg: 3.343 ± 0.505
5.596GluSer: 5.596 ± 0.678
3.415GluThr: 3.415 ± 0.516
6.904GluVal: 6.904 ± 0.803
0.945GluTrp: 0.945 ± 0.302
3.052GluTyr: 3.052 ± 0.476
0.0GluXaa: 0.0 ± 0.0
Phe
1.453PheAla: 1.453 ± 0.409
0.581PheCys: 0.581 ± 0.225
3.415PheAsp: 3.415 ± 0.494
2.907PheGlu: 2.907 ± 0.392
1.453PhePhe: 1.453 ± 0.305
3.052PheGly: 3.052 ± 0.596
0.363PheHis: 0.363 ± 0.132
2.325PheIle: 2.325 ± 0.321
2.035PheLys: 2.035 ± 0.318
2.543PheLeu: 2.543 ± 0.383
1.163PheMet: 1.163 ± 0.328
1.381PheAsn: 1.381 ± 0.255
1.671PhePro: 1.671 ± 0.353
1.817PheGln: 1.817 ± 0.315
1.308PheArg: 1.308 ± 0.337
2.834PheSer: 2.834 ± 0.541
2.761PheThr: 2.761 ± 0.383
1.889PheVal: 1.889 ± 0.426
0.291PheTrp: 0.291 ± 0.131
1.163PheTyr: 1.163 ± 0.22
0.0PheXaa: 0.0 ± 0.0
Gly
4.505GlyAla: 4.505 ± 0.651
1.017GlyCys: 1.017 ± 0.307
4.578GlyAsp: 4.578 ± 0.582
4.505GlyGlu: 4.505 ± 0.649
3.197GlyPhe: 3.197 ± 0.475
5.741GlyGly: 5.741 ± 0.743
0.799GlyHis: 0.799 ± 0.229
2.907GlyIle: 2.907 ± 0.515
5.45GlyLys: 5.45 ± 0.733
5.523GlyLeu: 5.523 ± 0.485
2.035GlyMet: 2.035 ± 0.296
3.706GlyAsn: 3.706 ± 0.574
0.945GlyPro: 0.945 ± 0.29
1.962GlyGln: 1.962 ± 0.323
3.125GlyArg: 3.125 ± 0.365
6.322GlySer: 6.322 ± 0.957
6.54GlyThr: 6.54 ± 0.771
4.578GlyVal: 4.578 ± 0.523
1.017GlyTrp: 1.017 ± 0.293
3.561GlyTyr: 3.561 ± 0.485
0.0GlyXaa: 0.0 ± 0.0
His
0.581HisAla: 0.581 ± 0.228
0.581HisCys: 0.581 ± 0.244
1.308HisAsp: 1.308 ± 0.29
1.09HisGlu: 1.09 ± 0.329
0.291HisPhe: 0.291 ± 0.141
1.235HisGly: 1.235 ± 0.353
0.436HisHis: 0.436 ± 0.201
0.654HisIle: 0.654 ± 0.207
1.453HisLys: 1.453 ± 0.261
1.744HisLeu: 1.744 ± 0.289
0.218HisMet: 0.218 ± 0.114
1.017HisAsn: 1.017 ± 0.246
0.799HisPro: 0.799 ± 0.224
0.727HisGln: 0.727 ± 0.171
1.599HisArg: 1.599 ± 0.321
1.526HisSer: 1.526 ± 0.321
1.381HisThr: 1.381 ± 0.372
1.381HisVal: 1.381 ± 0.344
0.291HisTrp: 0.291 ± 0.143
0.872HisTyr: 0.872 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
3.27IleAla: 3.27 ± 0.542
0.436IleCys: 0.436 ± 0.196
2.689IleAsp: 2.689 ± 0.363
3.706IleGlu: 3.706 ± 0.572
1.163IlePhe: 1.163 ± 0.253
3.561IleGly: 3.561 ± 0.487
1.09IleHis: 1.09 ± 0.292
2.689IleIle: 2.689 ± 0.388
3.851IleLys: 3.851 ± 0.531
2.907IleLeu: 2.907 ± 0.471
0.581IleMet: 0.581 ± 0.154
3.052IleAsn: 3.052 ± 0.435
2.761IlePro: 2.761 ± 0.4
3.197IleGln: 3.197 ± 0.693
3.27IleArg: 3.27 ± 0.409
3.924IleSer: 3.924 ± 0.498
3.27IleThr: 3.27 ± 0.516
2.761IleVal: 2.761 ± 0.407
0.581IleTrp: 0.581 ± 0.204
1.163IleTyr: 1.163 ± 0.252
0.0IleXaa: 0.0 ± 0.0
Lys
5.232LysAla: 5.232 ± 0.713
0.436LysCys: 0.436 ± 0.172
3.924LysAsp: 3.924 ± 0.532
4.651LysGlu: 4.651 ± 0.661
2.253LysPhe: 2.253 ± 0.382
4.505LysGly: 4.505 ± 0.562
1.235LysHis: 1.235 ± 0.269
2.689LysIle: 2.689 ± 0.375
3.197LysLys: 3.197 ± 0.629
5.814LysLeu: 5.814 ± 0.712
1.453LysMet: 1.453 ± 0.244
1.744LysAsn: 1.744 ± 0.203
3.27LysPro: 3.27 ± 0.512
2.253LysGln: 2.253 ± 0.298
2.471LysArg: 2.471 ± 0.466
4.142LysSer: 4.142 ± 0.448
3.415LysThr: 3.415 ± 0.482
5.087LysVal: 5.087 ± 0.541
0.581LysTrp: 0.581 ± 0.206
2.616LysTyr: 2.616 ± 0.484
0.0LysXaa: 0.0 ± 0.0
Leu
6.686LeuAla: 6.686 ± 0.881
0.436LeuCys: 0.436 ± 0.158
5.232LeuAsp: 5.232 ± 0.579
6.395LeuGlu: 6.395 ± 0.728
2.979LeuPhe: 2.979 ± 0.445
6.104LeuGly: 6.104 ± 0.737
1.526LeuHis: 1.526 ± 0.356
3.997LeuIle: 3.997 ± 0.528
4.942LeuLys: 4.942 ± 0.506
5.886LeuLeu: 5.886 ± 0.803
2.325LeuMet: 2.325 ± 0.37
4.942LeuAsn: 4.942 ± 0.552
2.979LeuPro: 2.979 ± 0.471
3.779LeuGln: 3.779 ± 0.595
4.651LeuArg: 4.651 ± 0.584
5.814LeuSer: 5.814 ± 0.735
5.668LeuThr: 5.668 ± 0.558
4.869LeuVal: 4.869 ± 0.596
1.453LeuTrp: 1.453 ± 0.367
2.761LeuTyr: 2.761 ± 0.475
0.0LeuXaa: 0.0 ± 0.0
Met
1.889MetAla: 1.889 ± 0.488
0.291MetCys: 0.291 ± 0.153
1.599MetAsp: 1.599 ± 0.348
1.599MetGlu: 1.599 ± 0.335
1.599MetPhe: 1.599 ± 0.411
0.581MetGly: 0.581 ± 0.169
0.654MetHis: 0.654 ± 0.249
1.744MetIle: 1.744 ± 0.436
1.526MetLys: 1.526 ± 0.304
2.035MetLeu: 2.035 ± 0.422
0.581MetMet: 0.581 ± 0.179
0.727MetAsn: 0.727 ± 0.218
1.017MetPro: 1.017 ± 0.284
2.035MetGln: 2.035 ± 0.402
1.017MetArg: 1.017 ± 0.229
2.18MetSer: 2.18 ± 0.297
1.163MetThr: 1.163 ± 0.25
0.872MetVal: 0.872 ± 0.327
0.363MetTrp: 0.363 ± 0.148
1.017MetTyr: 1.017 ± 0.271
0.0MetXaa: 0.0 ± 0.0
Asn
3.197AsnAla: 3.197 ± 0.433
1.017AsnCys: 1.017 ± 0.31
2.689AsnAsp: 2.689 ± 0.478
2.398AsnGlu: 2.398 ± 0.346
1.235AsnPhe: 1.235 ± 0.24
3.27AsnGly: 3.27 ± 0.615
0.872AsnHis: 0.872 ± 0.267
2.616AsnIle: 2.616 ± 0.516
3.125AsnLys: 3.125 ± 0.408
3.633AsnLeu: 3.633 ± 0.639
0.727AsnMet: 0.727 ± 0.215
1.744AsnAsn: 1.744 ± 0.312
2.035AsnPro: 2.035 ± 0.432
1.889AsnGln: 1.889 ± 0.375
2.035AsnArg: 2.035 ± 0.359
3.27AsnSer: 3.27 ± 0.681
2.616AsnThr: 2.616 ± 0.398
2.398AsnVal: 2.398 ± 0.426
0.727AsnTrp: 0.727 ± 0.243
2.035AsnTyr: 2.035 ± 0.362
0.0AsnXaa: 0.0 ± 0.0
Pro
1.962ProAla: 1.962 ± 0.421
0.291ProCys: 0.291 ± 0.151
3.415ProAsp: 3.415 ± 0.651
5.378ProGlu: 5.378 ± 0.515
1.599ProPhe: 1.599 ± 0.34
1.09ProGly: 1.09 ± 0.208
0.872ProHis: 0.872 ± 0.278
1.163ProIle: 1.163 ± 0.291
1.889ProLys: 1.889 ± 0.417
3.27ProLeu: 3.27 ± 0.449
0.654ProMet: 0.654 ± 0.212
2.325ProAsn: 2.325 ± 0.469
1.526ProPro: 1.526 ± 0.37
0.872ProGln: 0.872 ± 0.198
1.235ProArg: 1.235 ± 0.484
3.052ProSer: 3.052 ± 0.516
2.398ProThr: 2.398 ± 0.323
2.543ProVal: 2.543 ± 0.441
0.581ProTrp: 0.581 ± 0.173
1.09ProTyr: 1.09 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
4.142GlnAla: 4.142 ± 0.726
0.291GlnCys: 0.291 ± 0.139
3.851GlnAsp: 3.851 ± 0.486
3.125GlnGlu: 3.125 ± 0.453
1.308GlnPhe: 1.308 ± 0.287
2.907GlnGly: 2.907 ± 0.643
0.727GlnHis: 0.727 ± 0.238
1.817GlnIle: 1.817 ± 0.369
1.526GlnLys: 1.526 ± 0.283
3.706GlnLeu: 3.706 ± 0.49
1.235GlnMet: 1.235 ± 0.248
1.308GlnAsn: 1.308 ± 0.298
1.599GlnPro: 1.599 ± 0.343
1.889GlnGln: 1.889 ± 0.406
3.125GlnArg: 3.125 ± 0.479
2.689GlnSer: 2.689 ± 0.436
2.325GlnThr: 2.325 ± 0.454
3.052GlnVal: 3.052 ± 0.515
0.436GlnTrp: 0.436 ± 0.161
2.035GlnTyr: 2.035 ± 0.5
0.0GlnXaa: 0.0 ± 0.0
Arg
3.27ArgAla: 3.27 ± 0.502
0.509ArgCys: 0.509 ± 0.185
2.834ArgAsp: 2.834 ± 0.573
4.069ArgGlu: 4.069 ± 0.572
1.526ArgPhe: 1.526 ± 0.406
3.125ArgGly: 3.125 ± 0.457
1.09ArgHis: 1.09 ± 0.315
3.197ArgIle: 3.197 ± 0.587
3.488ArgLys: 3.488 ± 0.512
4.287ArgLeu: 4.287 ± 0.545
1.817ArgMet: 1.817 ± 0.47
2.18ArgAsn: 2.18 ± 0.372
1.962ArgPro: 1.962 ± 0.387
1.599ArgGln: 1.599 ± 0.423
3.052ArgArg: 3.052 ± 0.4
2.834ArgSer: 2.834 ± 0.342
4.215ArgThr: 4.215 ± 0.572
2.979ArgVal: 2.979 ± 0.413
0.581ArgTrp: 0.581 ± 0.18
2.107ArgTyr: 2.107 ± 0.375
0.0ArgXaa: 0.0 ± 0.0
Ser
4.578SerAla: 4.578 ± 0.787
0.727SerCys: 0.727 ± 0.226
4.578SerAsp: 4.578 ± 0.482
3.561SerGlu: 3.561 ± 0.461
2.253SerPhe: 2.253 ± 0.433
6.613SerGly: 6.613 ± 0.882
1.09SerHis: 1.09 ± 0.324
4.578SerIle: 4.578 ± 0.526
3.924SerLys: 3.924 ± 0.641
5.523SerLeu: 5.523 ± 0.61
1.671SerMet: 1.671 ± 0.269
3.706SerAsn: 3.706 ± 0.578
2.907SerPro: 2.907 ± 0.366
2.398SerGln: 2.398 ± 0.478
4.069SerArg: 4.069 ± 0.471
5.741SerSer: 5.741 ± 0.77
6.395SerThr: 6.395 ± 0.808
4.942SerVal: 4.942 ± 0.495
1.163SerTrp: 1.163 ± 0.358
2.471SerTyr: 2.471 ± 0.425
0.0SerXaa: 0.0 ± 0.0
Thr
4.433ThrAla: 4.433 ± 0.614
0.945ThrCys: 0.945 ± 0.293
4.215ThrAsp: 4.215 ± 0.365
4.578ThrGlu: 4.578 ± 0.577
2.616ThrPhe: 2.616 ± 0.366
6.032ThrGly: 6.032 ± 0.708
1.453ThrHis: 1.453 ± 0.267
2.18ThrIle: 2.18 ± 0.371
4.215ThrLys: 4.215 ± 0.705
6.831ThrLeu: 6.831 ± 0.741
1.163ThrMet: 1.163 ± 0.324
2.107ThrAsn: 2.107 ± 0.331
2.107ThrPro: 2.107 ± 0.425
2.979ThrGln: 2.979 ± 0.548
2.543ThrArg: 2.543 ± 0.357
5.305ThrSer: 5.305 ± 0.612
3.415ThrThr: 3.415 ± 0.61
4.287ThrVal: 4.287 ± 0.656
1.017ThrTrp: 1.017 ± 0.316
2.325ThrTyr: 2.325 ± 0.434
0.0ThrXaa: 0.0 ± 0.0
Val
4.287ValAla: 4.287 ± 0.592
0.727ValCys: 0.727 ± 0.303
3.488ValAsp: 3.488 ± 0.468
4.36ValGlu: 4.36 ± 0.515
2.979ValPhe: 2.979 ± 0.378
4.505ValGly: 4.505 ± 0.451
1.453ValHis: 1.453 ± 0.292
3.779ValIle: 3.779 ± 0.493
3.706ValLys: 3.706 ± 0.652
5.741ValLeu: 5.741 ± 0.707
1.671ValMet: 1.671 ± 0.333
2.907ValAsn: 2.907 ± 0.495
2.761ValPro: 2.761 ± 0.354
4.796ValGln: 4.796 ± 0.544
3.633ValArg: 3.633 ± 0.542
4.142ValSer: 4.142 ± 0.588
3.561ValThr: 3.561 ± 0.45
4.215ValVal: 4.215 ± 0.566
0.872ValTrp: 0.872 ± 0.215
3.27ValTyr: 3.27 ± 0.681
0.0ValXaa: 0.0 ± 0.0
Trp
0.799TrpAla: 0.799 ± 0.23
0.218TrpCys: 0.218 ± 0.193
1.017TrpAsp: 1.017 ± 0.243
1.09TrpGlu: 1.09 ± 0.273
0.363TrpPhe: 0.363 ± 0.155
0.872TrpGly: 0.872 ± 0.214
0.363TrpHis: 0.363 ± 0.177
0.363TrpIle: 0.363 ± 0.18
1.163TrpLys: 1.163 ± 0.187
0.799TrpLeu: 0.799 ± 0.306
0.509TrpMet: 0.509 ± 0.184
0.799TrpAsn: 0.799 ± 0.243
0.654TrpPro: 0.654 ± 0.242
0.727TrpGln: 0.727 ± 0.277
0.799TrpArg: 0.799 ± 0.205
0.581TrpSer: 0.581 ± 0.216
0.945TrpThr: 0.945 ± 0.336
0.872TrpVal: 0.872 ± 0.274
0.145TrpTrp: 0.145 ± 0.107
1.017TrpTyr: 1.017 ± 0.296
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.889TyrAla: 1.889 ± 0.304
0.436TyrCys: 0.436 ± 0.162
4.36TyrAsp: 4.36 ± 0.591
2.979TyrGlu: 2.979 ± 0.568
1.308TyrPhe: 1.308 ± 0.278
2.471TyrGly: 2.471 ± 0.382
1.235TyrHis: 1.235 ± 0.354
1.889TyrIle: 1.889 ± 0.362
2.18TyrLys: 2.18 ± 0.411
3.924TyrLeu: 3.924 ± 0.4
1.09TyrMet: 1.09 ± 0.215
2.253TyrAsn: 2.253 ± 0.407
1.235TyrPro: 1.235 ± 0.311
1.163TyrGln: 1.163 ± 0.255
2.471TyrArg: 2.471 ± 0.336
2.253TyrSer: 2.253 ± 0.377
2.253TyrThr: 2.253 ± 0.429
2.689TyrVal: 2.689 ± 0.418
0.509TyrTrp: 0.509 ± 0.185
1.817TyrTyr: 1.817 ± 0.436
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13762 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski