Leptospira phage vB_LnoZ_CZ214-LE1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5VKS6|S5VKS6_9CAUD Uncharacterized protein OS=Leptospira phage vB_LnoZ_CZ214-LE1 OX=1334243 GN=LEP1GSC059_0062 PE=4 SV=1
MM1 pKa = 7.63NNLLDD6 pKa = 5.16KK7 pKa = 11.01LPQFNSTDD15 pKa = 3.45PTFQCLWGDD24 pKa = 3.9LDD26 pKa = 4.54RR27 pKa = 11.84PLISPVTNINDD38 pKa = 3.43INKK41 pKa = 8.39GAMYY45 pKa = 10.76NSVEE49 pKa = 3.83WHH51 pKa = 6.99LLFQNLASEE60 pKa = 4.82CSTLTQSEE68 pKa = 4.65GRR70 pKa = 11.84FLTKK74 pKa = 9.76WAILLGIEE82 pKa = 4.8RR83 pKa = 11.84PTGMNDD89 pKa = 3.21PEE91 pKa = 4.22FVGYY95 pKa = 10.12ILGYY99 pKa = 9.88VLSNEE104 pKa = 3.91PTLTKK109 pKa = 10.11ISQIFQRR116 pKa = 11.84PDD118 pKa = 3.2YY119 pKa = 11.12AVLRR123 pKa = 11.84CNEE126 pKa = 4.2LGFTSEE132 pKa = 4.45VSATDD137 pKa = 2.89TGLFLPGPNTKK148 pKa = 8.69TVSSIVTPLLGVSYY162 pKa = 10.71ILVEE166 pKa = 4.27DD167 pKa = 4.7FSFISNVQITEE178 pKa = 4.05LNRR181 pKa = 11.84ILAAGTAVYY190 pKa = 10.1IGAKK194 pKa = 9.87NADD197 pKa = 3.39

Molecular weight: 21.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5VT39|S5VT39_9CAUD Uncharacterized protein OS=Leptospira phage vB_LnoZ_CZ214-LE1 OX=1334243 GN=LEP1GSC059_0064 PE=4 SV=1
MM1 pKa = 7.6AMSNKK6 pKa = 10.03SGGGGGILIKK16 pKa = 9.93EE17 pKa = 4.04IPGGYY22 pKa = 8.86RR23 pKa = 11.84VFHH26 pKa = 6.41PNKK29 pKa = 9.86GRR31 pKa = 11.84YY32 pKa = 9.27NYY34 pKa = 9.37MAVIEE39 pKa = 4.42KK40 pKa = 10.18GRR42 pKa = 11.84PRR44 pKa = 11.84YY45 pKa = 10.35DD46 pKa = 3.06MRR48 pKa = 11.84PALLGGSRR56 pKa = 11.84ARR58 pKa = 11.84MGKK61 pKa = 9.28NGPYY65 pKa = 10.16VIVPITKK72 pKa = 10.32NEE74 pKa = 4.34DD75 pKa = 3.14GTPLSFKK82 pKa = 10.75KK83 pKa = 9.48NTINSVIIKK92 pKa = 8.72TGSFKK97 pKa = 11.06EE98 pKa = 4.34EE99 pKa = 3.92NAHH102 pKa = 5.49GQLVTRR108 pKa = 11.84NKK110 pKa = 10.06YY111 pKa = 10.02KK112 pKa = 10.45YY113 pKa = 9.69RR114 pKa = 11.84QDD116 pKa = 3.31PGMTRR121 pKa = 11.84QGNVFLRR128 pKa = 11.84EE129 pKa = 3.77QTYY132 pKa = 11.36KK133 pKa = 10.88NGTVQRR139 pKa = 11.84SLVKK143 pKa = 9.32FVVVNEE149 pKa = 4.13RR150 pKa = 11.84SRR152 pKa = 11.84DD153 pKa = 3.63FFQAAIPAQKK163 pKa = 10.17VFSGVKK169 pKa = 9.88EE170 pKa = 4.09DD171 pKa = 3.42VKK173 pKa = 11.03KK174 pKa = 10.77ALKK177 pKa = 10.41SKK179 pKa = 8.09QLKK182 pKa = 9.72KK183 pKa = 10.74AVALDD188 pKa = 3.59TKK190 pKa = 11.15DD191 pKa = 3.89LIKK194 pKa = 10.76EE195 pKa = 4.04LLSKK199 pKa = 10.25KK200 pKa = 10.23RR201 pKa = 11.84KK202 pKa = 9.0

Molecular weight: 22.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

24976

40

2298

257.5

29.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.056 ± 0.486

0.889 ± 0.08

4.472 ± 0.16

7.383 ± 0.34

5.125 ± 0.297

5.854 ± 0.395

1.401 ± 0.098

7.884 ± 0.352

8.368 ± 0.464

10.402 ± 0.491

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.489 ± 0.085

7.223 ± 0.288

3.503 ± 0.186

4.356 ± 0.321

3.952 ± 0.212

8.108 ± 0.257

5.545 ± 0.247

4.997 ± 0.199

1.021 ± 0.067

3.972 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski