Amino acid dipepetide frequency for Alkalibacterium gilvum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.497AlaAla: 4.497 ± 0.104
0.369AlaCys: 0.369 ± 0.028
3.364AlaAsp: 3.364 ± 0.08
4.533AlaGlu: 4.533 ± 0.098
3.057AlaPhe: 3.057 ± 0.073
4.424AlaGly: 4.424 ± 0.108
1.223AlaHis: 1.223 ± 0.044
5.545AlaIle: 5.545 ± 0.097
4.281AlaLys: 4.281 ± 0.091
6.936AlaLeu: 6.936 ± 0.122
1.862AlaMet: 1.862 ± 0.064
2.674AlaAsn: 2.674 ± 0.076
1.669AlaPro: 1.669 ± 0.07
2.064AlaGln: 2.064 ± 0.06
2.429AlaArg: 2.429 ± 0.066
3.932AlaSer: 3.932 ± 0.076
3.332AlaThr: 3.332 ± 0.079
4.671AlaVal: 4.671 ± 0.094
0.455AlaTrp: 0.455 ± 0.03
2.416AlaTyr: 2.416 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.25CysAla: 0.25 ± 0.024
0.037CysCys: 0.037 ± 0.009
0.26CysAsp: 0.26 ± 0.022
0.318CysGlu: 0.318 ± 0.026
0.226CysPhe: 0.226 ± 0.021
0.427CysGly: 0.427 ± 0.028
0.135CysHis: 0.135 ± 0.015
0.302CysIle: 0.302 ± 0.021
0.224CysLys: 0.224 ± 0.02
0.38CysLeu: 0.38 ± 0.026
0.102CysMet: 0.102 ± 0.014
0.167CysAsn: 0.167 ± 0.014
0.227CysPro: 0.227 ± 0.021
0.149CysGln: 0.149 ± 0.016
0.175CysArg: 0.175 ± 0.02
0.333CysSer: 0.333 ± 0.025
0.24CysThr: 0.24 ± 0.019
0.271CysVal: 0.271 ± 0.021
0.029CysTrp: 0.029 ± 0.007
0.153CysTyr: 0.153 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.497AspAla: 3.497 ± 0.094
0.237AspCys: 0.237 ± 0.019
3.26AspAsp: 3.26 ± 0.087
5.234AspGlu: 5.234 ± 0.111
2.736AspPhe: 2.736 ± 0.066
3.356AspGly: 3.356 ± 0.086
1.055AspHis: 1.055 ± 0.045
5.002AspIle: 5.002 ± 0.102
4.408AspLys: 4.408 ± 0.094
5.421AspLeu: 5.421 ± 0.111
1.664AspMet: 1.664 ± 0.055
2.671AspAsn: 2.671 ± 0.069
2.008AspPro: 2.008 ± 0.062
1.981AspGln: 1.981 ± 0.063
2.331AspArg: 2.331 ± 0.067
3.419AspSer: 3.419 ± 0.088
3.33AspThr: 3.33 ± 0.075
4.216AspVal: 4.216 ± 0.087
0.656AspTrp: 0.656 ± 0.035
2.82AspTyr: 2.82 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
5.561GluAla: 5.561 ± 0.108
0.289GluCys: 0.289 ± 0.023
4.549GluAsp: 4.549 ± 0.104
7.923GluGlu: 7.923 ± 0.17
2.4GluPhe: 2.4 ± 0.062
4.291GluGly: 4.291 ± 0.091
1.343GluHis: 1.343 ± 0.045
5.712GluIle: 5.712 ± 0.097
7.443GluLys: 7.443 ± 0.131
7.233GluLeu: 7.233 ± 0.133
2.426GluMet: 2.426 ± 0.065
4.785GluAsn: 4.785 ± 0.094
2.002GluPro: 2.002 ± 0.066
2.96GluGln: 2.96 ± 0.072
3.538GluArg: 3.538 ± 0.093
4.203GluSer: 4.203 ± 0.093
4.801GluThr: 4.801 ± 0.101
5.028GluVal: 5.028 ± 0.085
0.773GluTrp: 0.773 ± 0.034
2.495GluTyr: 2.495 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
2.487PheAla: 2.487 ± 0.075
0.159PheCys: 0.159 ± 0.014
2.823PheAsp: 2.823 ± 0.065
2.952PheGlu: 2.952 ± 0.07
2.294PhePhe: 2.294 ± 0.084
3.099PheGly: 3.099 ± 0.077
0.705PheHis: 0.705 ± 0.034
3.793PheIle: 3.793 ± 0.102
3.028PheLys: 3.028 ± 0.076
4.181PheLeu: 4.181 ± 0.112
1.101PheMet: 1.101 ± 0.046
2.294PheAsn: 2.294 ± 0.063
1.406PhePro: 1.406 ± 0.051
1.416PheGln: 1.416 ± 0.042
1.3PheArg: 1.3 ± 0.05
3.562PheSer: 3.562 ± 0.087
2.578PheThr: 2.578 ± 0.062
2.879PheVal: 2.879 ± 0.076
0.369PheTrp: 0.369 ± 0.025
1.768PheTyr: 1.768 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
4.187GlyAla: 4.187 ± 0.107
0.318GlyCys: 0.318 ± 0.025
3.215GlyAsp: 3.215 ± 0.086
4.397GlyGlu: 4.397 ± 0.099
3.156GlyPhe: 3.156 ± 0.076
4.207GlyGly: 4.207 ± 0.111
1.286GlyHis: 1.286 ± 0.046
5.343GlyIle: 5.343 ± 0.111
4.54GlyLys: 4.54 ± 0.092
6.308GlyLeu: 6.308 ± 0.108
1.89GlyMet: 1.89 ± 0.056
2.595GlyAsn: 2.595 ± 0.071
1.422GlyPro: 1.422 ± 0.047
1.987GlyGln: 1.987 ± 0.067
2.494GlyArg: 2.494 ± 0.069
3.832GlySer: 3.832 ± 0.087
3.986GlyThr: 3.986 ± 0.085
4.551GlyVal: 4.551 ± 0.098
0.645GlyTrp: 0.645 ± 0.037
2.801GlyTyr: 2.801 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.197HisAla: 1.197 ± 0.046
0.112HisCys: 0.112 ± 0.015
1.029HisAsp: 1.029 ± 0.037
1.294HisGlu: 1.294 ± 0.046
0.976HisPhe: 0.976 ± 0.043
1.216HisGly: 1.216 ± 0.04
0.476HisHis: 0.476 ± 0.028
1.414HisIle: 1.414 ± 0.054
1.07HisLys: 1.07 ± 0.043
1.95HisLeu: 1.95 ± 0.058
0.448HisMet: 0.448 ± 0.027
0.807HisAsn: 0.807 ± 0.036
1.0HisPro: 1.0 ± 0.041
0.653HisGln: 0.653 ± 0.033
0.737HisArg: 0.737 ± 0.032
1.263HisSer: 1.263 ± 0.05
1.029HisThr: 1.029 ± 0.035
1.216HisVal: 1.216 ± 0.045
0.167HisTrp: 0.167 ± 0.018
0.896HisTyr: 0.896 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
5.446IleAla: 5.446 ± 0.101
0.359IleCys: 0.359 ± 0.028
5.183IleAsp: 5.183 ± 0.099
6.277IleGlu: 6.277 ± 0.108
3.255IlePhe: 3.255 ± 0.085
5.295IleGly: 5.295 ± 0.104
1.398IleHis: 1.398 ± 0.05
6.132IleIle: 6.132 ± 0.12
5.597IleLys: 5.597 ± 0.118
7.261IleLeu: 7.261 ± 0.121
1.914IleMet: 1.914 ± 0.064
3.869IleAsn: 3.869 ± 0.08
3.111IlePro: 3.111 ± 0.068
2.547IleGln: 2.547 ± 0.067
2.945IleArg: 2.945 ± 0.069
5.062IleSer: 5.062 ± 0.096
4.221IleThr: 4.221 ± 0.078
5.494IleVal: 5.494 ± 0.113
0.511IleTrp: 0.511 ± 0.033
2.614IleTyr: 2.614 ± 0.076
0.0IleXaa: 0.0 ± 0.0
Lys
4.848LysAla: 4.848 ± 0.086
0.205LysCys: 0.205 ± 0.021
4.788LysAsp: 4.788 ± 0.106
8.212LysGlu: 8.212 ± 0.126
1.791LysPhe: 1.791 ± 0.051
4.421LysGly: 4.421 ± 0.093
1.429LysHis: 1.429 ± 0.048
4.768LysIle: 4.768 ± 0.092
7.126LysLys: 7.126 ± 0.126
5.761LysLeu: 5.761 ± 0.102
2.195LysMet: 2.195 ± 0.063
4.536LysAsn: 4.536 ± 0.089
2.174LysPro: 2.174 ± 0.071
3.228LysGln: 3.228 ± 0.074
3.673LysArg: 3.673 ± 0.086
3.809LysSer: 3.809 ± 0.078
4.207LysThr: 4.207 ± 0.095
4.639LysVal: 4.639 ± 0.078
0.727LysTrp: 0.727 ± 0.037
2.429LysTyr: 2.429 ± 0.064
0.0LysXaa: 0.0 ± 0.0
Leu
6.288LeuAla: 6.288 ± 0.119
0.433LeuCys: 0.433 ± 0.029
5.709LeuAsp: 5.709 ± 0.099
6.957LeuGlu: 6.957 ± 0.095
4.846LeuPhe: 4.846 ± 0.113
5.808LeuGly: 5.808 ± 0.111
1.585LeuHis: 1.585 ± 0.041
7.311LeuIle: 7.311 ± 0.127
7.126LeuLys: 7.126 ± 0.117
9.511LeuLeu: 9.511 ± 0.194
2.57LeuMet: 2.57 ± 0.064
5.247LeuAsn: 5.247 ± 0.094
3.414LeuPro: 3.414 ± 0.08
2.577LeuGln: 2.577 ± 0.068
3.246LeuArg: 3.246 ± 0.078
7.642LeuSer: 7.642 ± 0.12
5.858LeuThr: 5.858 ± 0.098
6.075LeuVal: 6.075 ± 0.119
0.594LeuTrp: 0.594 ± 0.033
3.275LeuTyr: 3.275 ± 0.076
0.0LeuXaa: 0.0 ± 0.0
Met
1.848MetAla: 1.848 ± 0.055
0.112MetCys: 0.112 ± 0.014
1.765MetAsp: 1.765 ± 0.052
1.895MetGlu: 1.895 ± 0.062
0.901MetPhe: 0.901 ± 0.044
1.775MetGly: 1.775 ± 0.057
0.472MetHis: 0.472 ± 0.027
2.237MetIle: 2.237 ± 0.065
2.283MetLys: 2.283 ± 0.055
2.28MetLeu: 2.28 ± 0.058
0.758MetMet: 0.758 ± 0.038
1.755MetAsn: 1.755 ± 0.05
0.932MetPro: 0.932 ± 0.036
0.864MetGln: 0.864 ± 0.037
1.104MetArg: 1.104 ± 0.043
1.814MetSer: 1.814 ± 0.053
1.783MetThr: 1.783 ± 0.051
1.593MetVal: 1.593 ± 0.048
0.157MetTrp: 0.157 ± 0.018
0.77MetTyr: 0.77 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.871AsnAla: 2.871 ± 0.067
0.232AsnCys: 0.232 ± 0.018
3.108AsnAsp: 3.108 ± 0.076
4.239AsnGlu: 4.239 ± 0.091
1.859AsnPhe: 1.859 ± 0.051
3.21AsnGly: 3.21 ± 0.068
1.132AsnHis: 1.132 ± 0.047
3.996AsnIle: 3.996 ± 0.085
4.238AsnLys: 4.238 ± 0.089
4.069AsnLeu: 4.069 ± 0.077
1.309AsnMet: 1.309 ± 0.042
2.884AsnAsn: 2.884 ± 0.085
1.997AsnPro: 1.997 ± 0.064
2.283AsnGln: 2.283 ± 0.062
2.177AsnArg: 2.177 ± 0.071
2.45AsnSer: 2.45 ± 0.061
2.569AsnThr: 2.569 ± 0.067
3.085AsnVal: 3.085 ± 0.073
0.562AsnTrp: 0.562 ± 0.033
2.221AsnTyr: 2.221 ± 0.059
0.0AsnXaa: 0.0 ± 0.0
Pro
1.851ProAla: 1.851 ± 0.055
0.151ProCys: 0.151 ± 0.016
2.073ProAsp: 2.073 ± 0.057
2.997ProGlu: 2.997 ± 0.078
1.666ProPhe: 1.666 ± 0.059
1.895ProGly: 1.895 ± 0.049
0.646ProHis: 0.646 ± 0.03
2.585ProIle: 2.585 ± 0.066
2.103ProLys: 2.103 ± 0.059
3.117ProLeu: 3.117 ± 0.08
0.823ProMet: 0.823 ± 0.038
1.635ProAsn: 1.635 ± 0.06
0.623ProPro: 0.623 ± 0.032
0.937ProGln: 0.937 ± 0.038
1.021ProArg: 1.021 ± 0.041
1.992ProSer: 1.992 ± 0.056
1.822ProThr: 1.822 ± 0.068
2.447ProVal: 2.447 ± 0.065
0.287ProTrp: 0.287 ± 0.023
1.344ProTyr: 1.344 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
2.543GlnAla: 2.543 ± 0.064
0.107GlnCys: 0.107 ± 0.013
1.752GlnAsp: 1.752 ± 0.06
2.583GlnGlu: 2.583 ± 0.072
1.429GlnPhe: 1.429 ± 0.051
1.846GlnGly: 1.846 ± 0.053
0.62GlnHis: 0.62 ± 0.029
2.338GlnIle: 2.338 ± 0.063
2.755GlnLys: 2.755 ± 0.075
3.876GlnLeu: 3.876 ± 0.084
0.976GlnMet: 0.976 ± 0.043
1.614GlnAsn: 1.614 ± 0.057
0.966GlnPro: 0.966 ± 0.036
1.266GlnGln: 1.266 ± 0.052
1.357GlnArg: 1.357 ± 0.059
2.289GlnSer: 2.289 ± 0.067
2.318GlnThr: 2.318 ± 0.071
2.09GlnVal: 2.09 ± 0.063
0.362GlnTrp: 0.362 ± 0.022
1.187GlnTyr: 1.187 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
2.239ArgAla: 2.239 ± 0.069
0.157ArgCys: 0.157 ± 0.016
1.994ArgAsp: 1.994 ± 0.062
3.075ArgGlu: 3.075 ± 0.083
1.955ArgPhe: 1.955 ± 0.056
2.161ArgGly: 2.161 ± 0.062
0.722ArgHis: 0.722 ± 0.031
3.169ArgIle: 3.169 ± 0.07
3.153ArgLys: 3.153 ± 0.075
3.957ArgLeu: 3.957 ± 0.08
1.258ArgMet: 1.258 ± 0.044
1.883ArgAsn: 1.883 ± 0.054
1.247ArgPro: 1.247 ± 0.048
1.554ArgGln: 1.554 ± 0.062
1.815ArgArg: 1.815 ± 0.064
2.291ArgSer: 2.291 ± 0.068
2.005ArgThr: 2.005 ± 0.057
2.57ArgVal: 2.57 ± 0.063
0.343ArgTrp: 0.343 ± 0.026
1.632ArgTyr: 1.632 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
3.671SerAla: 3.671 ± 0.082
0.252SerCys: 0.252 ± 0.021
3.903SerAsp: 3.903 ± 0.085
4.627SerGlu: 4.627 ± 0.091
3.211SerPhe: 3.211 ± 0.083
4.467SerGly: 4.467 ± 0.092
1.279SerHis: 1.279 ± 0.052
5.246SerIle: 5.246 ± 0.095
4.413SerLys: 4.413 ± 0.092
6.256SerLeu: 6.256 ± 0.117
1.615SerMet: 1.615 ± 0.051
3.018SerAsn: 3.018 ± 0.067
1.94SerPro: 1.94 ± 0.053
2.273SerGln: 2.273 ± 0.062
2.395SerArg: 2.395 ± 0.07
3.955SerSer: 3.955 ± 0.096
3.388SerThr: 3.388 ± 0.068
4.514SerVal: 4.514 ± 0.094
0.531SerTrp: 0.531 ± 0.029
2.369SerTyr: 2.369 ± 0.056
0.0SerXaa: 0.0 ± 0.0
Thr
3.53ThrAla: 3.53 ± 0.087
0.257ThrCys: 0.257 ± 0.02
3.471ThrAsp: 3.471 ± 0.081
4.153ThrGlu: 4.153 ± 0.105
2.919ThrPhe: 2.919 ± 0.068
4.014ThrGly: 4.014 ± 0.084
1.286ThrHis: 1.286 ± 0.05
4.772ThrIle: 4.772 ± 0.097
3.535ThrLys: 3.535 ± 0.077
6.204ThrLeu: 6.204 ± 0.111
1.313ThrMet: 1.313 ± 0.045
2.578ThrAsn: 2.578 ± 0.069
2.059ThrPro: 2.059 ± 0.059
1.841ThrGln: 1.841 ± 0.054
1.976ThrArg: 1.976 ± 0.065
3.512ThrSer: 3.512 ± 0.066
3.137ThrThr: 3.137 ± 0.07
4.205ThrVal: 4.205 ± 0.083
0.468ThrTrp: 0.468 ± 0.031
2.213ThrTyr: 2.213 ± 0.059
0.0ThrXaa: 0.0 ± 0.0
Val
4.293ValAla: 4.293 ± 0.101
0.378ValCys: 0.378 ± 0.028
4.142ValAsp: 4.142 ± 0.079
4.983ValGlu: 4.983 ± 0.086
3.072ValPhe: 3.072 ± 0.083
4.246ValGly: 4.246 ± 0.106
1.171ValHis: 1.171 ± 0.048
5.366ValIle: 5.366 ± 0.109
4.592ValLys: 4.592 ± 0.105
6.499ValLeu: 6.499 ± 0.098
1.786ValMet: 1.786 ± 0.058
3.302ValAsn: 3.302 ± 0.067
2.299ValPro: 2.299 ± 0.066
1.851ValGln: 1.851 ± 0.059
2.422ValArg: 2.422 ± 0.072
4.895ValSer: 4.895 ± 0.097
4.087ValThr: 4.087 ± 0.074
4.593ValVal: 4.593 ± 0.098
0.549ValTrp: 0.549 ± 0.031
2.364ValTyr: 2.364 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.442TrpAla: 0.442 ± 0.027
0.042TrpCys: 0.042 ± 0.008
0.521TrpAsp: 0.521 ± 0.027
0.572TrpGlu: 0.572 ± 0.033
0.459TrpPhe: 0.459 ± 0.031
0.5TrpGly: 0.5 ± 0.033
0.154TrpHis: 0.154 ± 0.017
0.695TrpIle: 0.695 ± 0.035
0.576TrpLys: 0.576 ± 0.033
0.994TrpLeu: 0.994 ± 0.04
0.287TrpMet: 0.287 ± 0.025
0.421TrpAsn: 0.421 ± 0.024
0.203TrpPro: 0.203 ± 0.024
0.341TrpGln: 0.341 ± 0.023
0.372TrpArg: 0.372 ± 0.023
0.523TrpSer: 0.523 ± 0.034
0.529TrpThr: 0.529 ± 0.029
0.536TrpVal: 0.536 ± 0.033
0.099TrpTrp: 0.099 ± 0.014
0.321TrpTyr: 0.321 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.189TyrAla: 2.189 ± 0.059
0.2TyrCys: 0.2 ± 0.018
2.396TyrAsp: 2.396 ± 0.068
2.533TyrGlu: 2.533 ± 0.063
1.986TyrPhe: 1.986 ± 0.066
2.502TyrGly: 2.502 ± 0.069
0.835TyrHis: 0.835 ± 0.038
2.765TyrIle: 2.765 ± 0.073
2.476TyrLys: 2.476 ± 0.069
3.882TyrLeu: 3.882 ± 0.083
0.796TyrMet: 0.796 ± 0.034
1.752TyrAsn: 1.752 ± 0.053
1.315TyrPro: 1.315 ± 0.047
1.505TyrGln: 1.505 ± 0.055
1.672TyrArg: 1.672 ± 0.058
2.544TyrSer: 2.544 ± 0.059
2.221TyrThr: 2.221 ± 0.065
2.216TyrVal: 2.216 ± 0.052
0.304TyrTrp: 0.304 ± 0.024
1.588TyrTyr: 1.588 ± 0.049
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2101 proteins (615918 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski