Desulfotomaculum profundi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfotomaculum

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C6M6P6|A0A2C6M6P6_9FIRM 6-carboxy-5 6 7 8-tetrahydropterin synthase OS=Desulfotomaculum profundi OX=1383067 GN=P378_13440 PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 10.44AVVDD6 pKa = 3.61QDD8 pKa = 4.3LCISCGACIDD18 pKa = 3.69VCPEE22 pKa = 3.61VFDD25 pKa = 4.39WNDD28 pKa = 2.98EE29 pKa = 4.23EE30 pKa = 4.32KK31 pKa = 10.99AHH33 pKa = 6.83AIVDD37 pKa = 4.1EE38 pKa = 4.61VPDD41 pKa = 4.1GQEE44 pKa = 3.74DD45 pKa = 3.65QARR48 pKa = 11.84EE49 pKa = 4.14ATEE52 pKa = 4.22GCPTEE57 pKa = 4.67AIKK60 pKa = 10.57ISS62 pKa = 3.37

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C6MD83|A0A2C6MD83_9FIRM Uncharacterized protein OS=Desulfotomaculum profundi OX=1383067 GN=P378_17125 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.19SLGFNATLLAQLFNFIVLVVIMVVLGRR29 pKa = 11.84MACVLLTGRR38 pKa = 11.84TSKK41 pKa = 10.58NHH43 pKa = 6.01FGKK46 pKa = 9.9IEE48 pKa = 3.89EE49 pKa = 4.27EE50 pKa = 3.79LRR52 pKa = 11.84EE53 pKa = 3.98IRR55 pKa = 11.84TVWVRR60 pKa = 11.84LRR62 pKa = 11.84KK63 pKa = 9.17SWIKK67 pKa = 10.29VRR69 pKa = 11.84QLNKK73 pKa = 9.75SLEE76 pKa = 3.69RR77 pKa = 11.84RR78 pKa = 11.84YY79 pKa = 10.2YY80 pKa = 10.55VNNCIRR86 pKa = 3.59

Molecular weight: 10.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3299

0

3299

703881

29

1638

213.4

23.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.989 ± 0.052

1.292 ± 0.028

4.792 ± 0.037

7.009 ± 0.046

3.847 ± 0.035

7.392 ± 0.043

1.916 ± 0.023

6.831 ± 0.041

6.446 ± 0.047

10.274 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.676 ± 0.025

3.922 ± 0.033

4.283 ± 0.035

3.842 ± 0.036

5.434 ± 0.042

5.242 ± 0.039

5.103 ± 0.031

7.436 ± 0.047

1.06 ± 0.018

3.196 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski