Caproiciproducens sp. NJN-50
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3089 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410Q3Q7|A0A410Q3Q7_9FIRM DUF87 domain-containing protein OS=Caproiciproducens sp. NJN-50 OX=2507162 GN=EQM14_09010 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.05 KK3 pKa = 10.16 YY4 pKa = 10.52 RR5 pKa = 11.84 CIPCGYY11 pKa = 9.66 IYY13 pKa = 10.6 DD14 pKa = 4.44 PALGDD19 pKa = 3.93 PDD21 pKa = 4.82 SGIGPGTVFEE31 pKa = 5.37 DD32 pKa = 5.94 LPDD35 pKa = 4.02 DD36 pKa = 4.05 WQCPICFVGKK46 pKa = 10.37 DD47 pKa = 3.32 EE48 pKa = 4.82 FEE50 pKa = 4.39 PVEE53 pKa = 4.21 DD54 pKa = 3.8
Molecular weight: 6.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.846
Dawson 3.757
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 0.401
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A410PZ63|A0A410PZ63_9FIRM GNAT family N-acetyltransferase OS=Caproiciproducens sp. NJN-50 OX=2507162 GN=EQM14_00370 PE=4 SV=1
MM1 pKa = 7.0 QLAPRR6 pKa = 11.84 RR7 pKa = 11.84 AQDD10 pKa = 2.82 ASLAYY15 pKa = 9.98 GSHH18 pKa = 6.66 DD19 pKa = 4.08 CDD21 pKa = 3.75 RR22 pKa = 11.84 AACLAEE28 pKa = 3.96 PLALGRR34 pKa = 11.84 FFYY37 pKa = 9.37 GTAKK41 pKa = 10.56 APNGDD46 pKa = 3.59 SSVTLPGVSPVLSRR60 pKa = 11.84 RR61 pKa = 11.84 NLLRR65 pKa = 11.84 AKK67 pKa = 10.01 VEE69 pKa = 3.83 RR70 pKa = 11.84 AKK72 pKa = 10.82 DD73 pKa = 3.8 GRR75 pKa = 11.84 CSRR78 pKa = 11.84 KK79 pKa = 9.58 FMSLRR84 pKa = 11.84 PRR86 pKa = 11.84 PPP88 pKa = 3.03
Molecular weight: 9.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.589
IPC_protein 10.511
Toseland 10.701
ProMoST 10.467
Dawson 10.789
Bjellqvist 10.54
Wikipedia 11.023
Rodwell 10.906
Grimsley 10.833
Solomon 10.95
Lehninger 10.921
Nozaki 10.716
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.125
Sillero 10.745
Patrickios 10.687
IPC_peptide 10.965
IPC2_peptide 9.853
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3089
0
3089
945004
31
1435
305.9
33.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.912 ± 0.052
1.62 ± 0.017
5.328 ± 0.033
6.428 ± 0.048
4.172 ± 0.032
8.057 ± 0.048
1.663 ± 0.018
6.441 ± 0.042
5.639 ± 0.039
9.598 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.758 ± 0.021
3.716 ± 0.027
4.057 ± 0.031
3.152 ± 0.024
5.501 ± 0.044
6.516 ± 0.041
5.225 ± 0.041
7.02 ± 0.034
0.864 ± 0.014
3.331 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here