Krasilnikovia cinnamomea
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6517 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q7ZMT7|A0A4Q7ZMT7_9ACTN Uncharacterized protein OS=Krasilnikovia cinnamomea OX=349313 GN=EV385_3508 PE=4 SV=1
MM1 pKa = 7.1 TVSFDD6 pKa = 3.7 LHH8 pKa = 7.48 VLAIDD13 pKa = 4.43 PDD15 pKa = 3.93 ASDD18 pKa = 3.63 DD19 pKa = 3.67 AVRR22 pKa = 11.84 AMVDD26 pKa = 3.11 RR27 pKa = 11.84 CQGDD31 pKa = 3.14 AHH33 pKa = 7.99 AEE35 pKa = 4.14 GEE37 pKa = 4.1 LDD39 pKa = 3.42 QRR41 pKa = 11.84 IVDD44 pKa = 4.66 FYY46 pKa = 9.97 EE47 pKa = 3.96 SLRR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 YY53 pKa = 9.64 PDD55 pKa = 3.86 FPPYY59 pKa = 10.17 PDD61 pKa = 4.58 DD62 pKa = 4.47 SPWMSMPLDD71 pKa = 3.49 VGVDD75 pKa = 3.87 HH76 pKa = 7.16 VCMSMSFSSSDD87 pKa = 3.33 DD88 pKa = 3.68 VIYY91 pKa = 10.65 LIFDD95 pKa = 4.45 LAQQYY100 pKa = 10.13 EE101 pKa = 4.46 LTVYY105 pKa = 10.48 DD106 pKa = 4.22 PQDD109 pKa = 3.62 DD110 pKa = 4.19 SVARR114 pKa = 11.84 PGDD117 pKa = 3.61 DD118 pKa = 2.96
Molecular weight: 13.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.503
ProMoST 3.897
Dawson 3.77
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.567
Grimsley 3.414
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 4.202
Thurlkill 3.579
EMBOSS 3.757
Sillero 3.872
Patrickios 1.901
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A4Q7ZS29|A0A4Q7ZS29_9ACTN Uncharacterized protein OS=Krasilnikovia cinnamomea OX=349313 GN=EV385_5552 PE=4 SV=1
MM1 pKa = 7.72 KK2 pKa = 10.37 NGTVSGLRR10 pKa = 11.84 AQAPRR15 pKa = 11.84 RR16 pKa = 11.84 ARR18 pKa = 11.84 QAQRR22 pKa = 11.84 AVRR25 pKa = 11.84 NHH27 pKa = 6.59 PAPAAGVLALAGAVAAVLLGRR48 pKa = 11.84 RR49 pKa = 11.84 AAKK52 pKa = 10.1 SRR54 pKa = 11.84 SARR57 pKa = 11.84 KK58 pKa = 9.23 RR59 pKa = 11.84 RR60 pKa = 11.84 LPRR63 pKa = 11.84 LLHH66 pKa = 6.11 RR67 pKa = 5.13
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6517
0
6517
2234958
29
9930
342.9
36.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.629 ± 0.057
0.773 ± 0.009
6.062 ± 0.027
4.879 ± 0.03
2.624 ± 0.016
9.251 ± 0.031
2.205 ± 0.014
3.279 ± 0.022
1.726 ± 0.026
10.231 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.665 ± 0.011
1.804 ± 0.02
6.318 ± 0.036
2.889 ± 0.018
8.359 ± 0.035
4.736 ± 0.02
6.24 ± 0.031
8.756 ± 0.028
1.542 ± 0.013
2.031 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here