Microbacterium sp. SLBN-146

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A542FX14|A0A542FX14_9MICO DNA topoisomerase (ATP-hydrolyzing) OS=Microbacterium sp. SLBN-146 OX=2768457 GN=FBY39_2628 PE=3 SV=1
MM1 pKa = 7.31NSHH4 pKa = 6.25RR5 pKa = 11.84RR6 pKa = 11.84IAAVAFVAATLLTVAGCASGAPGGAEE32 pKa = 3.75PPGAGLGGVWPAPPDD47 pKa = 3.6VRR49 pKa = 11.84VMTQGTVLDD58 pKa = 4.09TNGDD62 pKa = 3.63VEE64 pKa = 4.57LCLGAVAEE72 pKa = 4.92SYY74 pKa = 10.6PPQCSGIPVVGWSWDD89 pKa = 3.51GVDD92 pKa = 4.59GSEE95 pKa = 4.58SANGVTWGAYY105 pKa = 8.39GVQGTYY111 pKa = 10.47DD112 pKa = 3.73GEE114 pKa = 4.69TFTVDD119 pKa = 3.28QPPVMLALYY128 pKa = 10.4DD129 pKa = 3.85PVKK132 pKa = 10.6PDD134 pKa = 3.85DD135 pKa = 3.96PTGGRR140 pKa = 11.84AGAGTEE146 pKa = 3.96EE147 pKa = 4.62QLAEE151 pKa = 4.32LQNDD155 pKa = 3.76LPDD158 pKa = 4.23RR159 pKa = 11.84LAEE162 pKa = 4.85GYY164 pKa = 7.7LTSYY168 pKa = 10.24IDD170 pKa = 5.39NGWLWVDD177 pKa = 3.77VVWDD181 pKa = 4.17DD182 pKa = 4.56GTWQAAADD190 pKa = 4.07EE191 pKa = 4.93EE192 pKa = 4.67YY193 pKa = 10.95GADD196 pKa = 3.39VVIIRR201 pKa = 11.84SVIRR205 pKa = 11.84PFF207 pKa = 3.52

Molecular weight: 21.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A542FU12|A0A542FU12_9MICO CAAX prenyl protease-like protein OS=Microbacterium sp. SLBN-146 OX=2768457 GN=FBY39_1509 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3245

0

3245

1108321

29

10428

341.5

36.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.538 ± 0.07

0.481 ± 0.009

6.504 ± 0.036

5.592 ± 0.047

3.136 ± 0.028

8.934 ± 0.053

1.905 ± 0.021

4.637 ± 0.029

1.721 ± 0.032

9.844 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.7 ± 0.021

1.918 ± 0.028

5.458 ± 0.034

2.562 ± 0.024

7.32 ± 0.062

5.826 ± 0.043

6.28 ± 0.07

9.127 ± 0.044

1.548 ± 0.022

1.968 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski