Amino acid dipepetide frequency for Microbacterium sp. SLBN-146

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.292AlaAla: 20.292 ± 0.215
0.714AlaCys: 0.714 ± 0.026
8.432AlaAsp: 8.432 ± 0.107
7.848AlaGlu: 7.848 ± 0.108
3.976AlaPhe: 3.976 ± 0.066
12.103AlaGly: 12.103 ± 0.198
2.413AlaHis: 2.413 ± 0.057
6.21AlaIle: 6.21 ± 0.088
2.386AlaLys: 2.386 ± 0.057
13.899AlaLeu: 13.899 ± 0.15
2.518AlaMet: 2.518 ± 0.055
2.355AlaAsn: 2.355 ± 0.061
6.731AlaPro: 6.731 ± 0.09
3.533AlaGln: 3.533 ± 0.059
9.512AlaArg: 9.512 ± 0.124
8.165AlaSer: 8.165 ± 0.119
7.931AlaThr: 7.931 ± 0.177
11.838AlaVal: 11.838 ± 0.14
2.073AlaTrp: 2.073 ± 0.045
2.454AlaTyr: 2.454 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.674CysAla: 0.674 ± 0.023
0.051CysCys: 0.051 ± 0.006
0.321CysAsp: 0.321 ± 0.017
0.261CysGlu: 0.261 ± 0.017
0.149CysPhe: 0.149 ± 0.012
0.533CysGly: 0.533 ± 0.026
0.117CysHis: 0.117 ± 0.011
0.192CysIle: 0.192 ± 0.013
0.048CysLys: 0.048 ± 0.007
0.411CysLeu: 0.411 ± 0.021
0.075CysMet: 0.075 ± 0.009
0.099CysAsn: 0.099 ± 0.01
0.254CysPro: 0.254 ± 0.017
0.116CysGln: 0.116 ± 0.012
0.356CysArg: 0.356 ± 0.019
0.289CysSer: 0.289 ± 0.017
0.306CysThr: 0.306 ± 0.018
0.394CysVal: 0.394 ± 0.02
0.075CysTrp: 0.075 ± 0.008
0.09CysTyr: 0.09 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
10.03AspAla: 10.03 ± 0.121
0.219AspCys: 0.219 ± 0.015
4.944AspAsp: 4.944 ± 0.088
4.43AspGlu: 4.43 ± 0.074
1.691AspPhe: 1.691 ± 0.034
6.325AspGly: 6.325 ± 0.097
1.199AspHis: 1.199 ± 0.032
2.644AspIle: 2.644 ± 0.05
0.861AspLys: 0.861 ± 0.032
6.316AspLeu: 6.316 ± 0.08
0.88AspMet: 0.88 ± 0.031
1.02AspAsn: 1.02 ± 0.034
4.39AspPro: 4.39 ± 0.077
1.354AspGln: 1.354 ± 0.037
4.615AspArg: 4.615 ± 0.093
2.686AspSer: 2.686 ± 0.049
3.128AspThr: 3.128 ± 0.061
6.149AspVal: 6.149 ± 0.082
0.918AspTrp: 0.918 ± 0.031
1.244AspTyr: 1.244 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
6.975GluAla: 6.975 ± 0.106
0.238GluCys: 0.238 ± 0.015
2.76GluAsp: 2.76 ± 0.059
3.075GluGlu: 3.075 ± 0.061
1.793GluPhe: 1.793 ± 0.041
4.305GluGly: 4.305 ± 0.07
1.306GluHis: 1.306 ± 0.04
2.805GluIle: 2.805 ± 0.052
1.275GluLys: 1.275 ± 0.046
6.159GluLeu: 6.159 ± 0.092
0.907GluMet: 0.907 ± 0.029
1.205GluAsn: 1.205 ± 0.034
3.079GluPro: 3.079 ± 0.065
1.879GluGln: 1.879 ± 0.048
5.196GluArg: 5.196 ± 0.094
3.186GluSer: 3.186 ± 0.06
3.245GluThr: 3.245 ± 0.056
4.354GluVal: 4.354 ± 0.077
0.986GluTrp: 0.986 ± 0.029
1.194GluTyr: 1.194 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
4.505PheAla: 4.505 ± 0.075
0.14PheCys: 0.14 ± 0.011
2.47PheAsp: 2.47 ± 0.059
1.721PheGlu: 1.721 ± 0.049
1.103PhePhe: 1.103 ± 0.034
3.399PheGly: 3.399 ± 0.058
0.551PheHis: 0.551 ± 0.027
1.218PheIle: 1.218 ± 0.037
0.391PheLys: 0.391 ± 0.019
2.813PheLeu: 2.813 ± 0.056
0.43PheMet: 0.43 ± 0.02
0.643PheAsn: 0.643 ± 0.024
1.4PhePro: 1.4 ± 0.035
0.731PheGln: 0.731 ± 0.027
1.842PheArg: 1.842 ± 0.048
1.74PheSer: 1.74 ± 0.037
2.205PheThr: 2.205 ± 0.049
2.89PheVal: 2.89 ± 0.057
0.529PheTrp: 0.529 ± 0.023
0.636PheTyr: 0.636 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
10.809GlyAla: 10.809 ± 0.196
0.539GlyCys: 0.539 ± 0.023
5.46GlyAsp: 5.46 ± 0.089
4.92GlyGlu: 4.92 ± 0.071
3.32GlyPhe: 3.32 ± 0.053
7.963GlyGly: 7.963 ± 0.172
1.732GlyHis: 1.732 ± 0.037
5.035GlyIle: 5.035 ± 0.069
1.856GlyLys: 1.856 ± 0.046
8.4GlyLeu: 8.4 ± 0.102
1.867GlyMet: 1.867 ± 0.045
1.717GlyAsn: 1.717 ± 0.048
3.836GlyPro: 3.836 ± 0.066
2.303GlyGln: 2.303 ± 0.044
6.28GlyArg: 6.28 ± 0.087
5.544GlySer: 5.544 ± 0.099
5.688GlyThr: 5.688 ± 0.107
8.267GlyVal: 8.267 ± 0.099
1.66GlyTrp: 1.66 ± 0.038
2.139GlyTyr: 2.139 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.382HisAla: 2.382 ± 0.05
0.115HisCys: 0.115 ± 0.011
1.322HisAsp: 1.322 ± 0.036
1.06HisGlu: 1.06 ± 0.034
0.544HisPhe: 0.544 ± 0.023
1.918HisGly: 1.918 ± 0.045
0.533HisHis: 0.533 ± 0.027
0.726HisIle: 0.726 ± 0.025
0.238HisLys: 0.238 ± 0.017
1.915HisLeu: 1.915 ± 0.047
0.29HisMet: 0.29 ± 0.016
0.323HisAsn: 0.323 ± 0.017
1.481HisPro: 1.481 ± 0.045
0.403HisGln: 0.403 ± 0.02
1.496HisArg: 1.496 ± 0.039
0.965HisSer: 0.965 ± 0.032
0.943HisThr: 0.943 ± 0.035
1.732HisVal: 1.732 ± 0.047
0.275HisTrp: 0.275 ± 0.016
0.386HisTyr: 0.386 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
7.319IleAla: 7.319 ± 0.091
0.208IleCys: 0.208 ± 0.014
3.802IleAsp: 3.802 ± 0.06
2.953IleGlu: 2.953 ± 0.053
1.259IlePhe: 1.259 ± 0.043
4.618IleGly: 4.618 ± 0.075
0.726IleHis: 0.726 ± 0.026
1.766IleIle: 1.766 ± 0.048
0.659IleLys: 0.659 ± 0.029
4.032IleLeu: 4.032 ± 0.072
0.556IleMet: 0.556 ± 0.024
0.844IleAsn: 0.844 ± 0.027
2.586IlePro: 2.586 ± 0.047
0.948IleGln: 0.948 ± 0.031
2.717IleArg: 2.717 ± 0.052
2.359IleSer: 2.359 ± 0.052
2.712IleThr: 2.712 ± 0.065
5.081IleVal: 5.081 ± 0.081
0.474IleTrp: 0.474 ± 0.02
0.756IleTyr: 0.756 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
2.058LysAla: 2.058 ± 0.053
0.049LysCys: 0.049 ± 0.007
1.039LysAsp: 1.039 ± 0.04
0.833LysGlu: 0.833 ± 0.034
0.409LysPhe: 0.409 ± 0.02
1.333LysGly: 1.333 ± 0.036
0.352LysHis: 0.352 ± 0.018
0.877LysIle: 0.877 ± 0.03
0.629LysLys: 0.629 ± 0.027
1.585LysLeu: 1.585 ± 0.042
0.283LysMet: 0.283 ± 0.018
0.437LysAsn: 0.437 ± 0.021
1.04LysPro: 1.04 ± 0.034
0.529LysGln: 0.529 ± 0.025
1.394LysArg: 1.394 ± 0.042
1.055LysSer: 1.055 ± 0.031
1.235LysThr: 1.235 ± 0.037
1.466LysVal: 1.466 ± 0.04
0.231LysTrp: 0.231 ± 0.014
0.382LysTyr: 0.382 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
14.516LeuAla: 14.516 ± 0.153
0.481LeuCys: 0.481 ± 0.022
6.707LeuAsp: 6.707 ± 0.091
5.123LeuGlu: 5.123 ± 0.085
2.826LeuPhe: 2.826 ± 0.06
8.79LeuGly: 8.79 ± 0.113
1.713LeuHis: 1.713 ± 0.038
4.305LeuIle: 4.305 ± 0.075
1.405LeuLys: 1.405 ± 0.041
9.618LeuLeu: 9.618 ± 0.144
1.5LeuMet: 1.5 ± 0.041
1.626LeuAsn: 1.626 ± 0.041
5.351LeuPro: 5.351 ± 0.066
2.318LeuGln: 2.318 ± 0.046
7.271LeuArg: 7.271 ± 0.119
5.94LeuSer: 5.94 ± 0.072
6.438LeuThr: 6.438 ± 0.087
9.498LeuVal: 9.498 ± 0.111
1.35LeuTrp: 1.35 ± 0.04
1.666LeuTyr: 1.666 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
1.999MetAla: 1.999 ± 0.045
0.09MetCys: 0.09 ± 0.008
0.817MetAsp: 0.817 ± 0.027
0.566MetGlu: 0.566 ± 0.025
0.466MetPhe: 0.466 ± 0.022
1.275MetGly: 1.275 ± 0.036
0.316MetHis: 0.316 ± 0.017
0.797MetIle: 0.797 ± 0.029
0.383MetLys: 0.383 ± 0.018
1.777MetLeu: 1.777 ± 0.044
0.31MetMet: 0.31 ± 0.018
0.453MetAsn: 0.453 ± 0.02
1.148MetPro: 1.148 ± 0.033
0.506MetGln: 0.506 ± 0.022
1.28MetArg: 1.28 ± 0.036
1.414MetSer: 1.414 ± 0.04
1.753MetThr: 1.753 ± 0.042
1.162MetVal: 1.162 ± 0.037
0.22MetTrp: 0.22 ± 0.015
0.272MetTyr: 0.272 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.487AsnAla: 2.487 ± 0.064
0.115AsnCys: 0.115 ± 0.01
1.184AsnAsp: 1.184 ± 0.04
0.996AsnGlu: 0.996 ± 0.031
0.628AsnPhe: 0.628 ± 0.031
1.91AsnGly: 1.91 ± 0.047
0.336AsnHis: 0.336 ± 0.016
0.842AsnIle: 0.842 ± 0.03
0.328AsnLys: 0.328 ± 0.019
1.859AsnLeu: 1.859 ± 0.045
0.285AsnMet: 0.285 ± 0.018
0.453AsnAsn: 0.453 ± 0.023
1.571AsnPro: 1.571 ± 0.039
0.495AsnGln: 0.495 ± 0.024
1.226AsnArg: 1.226 ± 0.033
0.884AsnSer: 0.884 ± 0.031
1.128AsnThr: 1.128 ± 0.041
1.694AsnVal: 1.694 ± 0.05
0.306AsnTrp: 0.306 ± 0.017
0.452AsnTyr: 0.452 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
7.28ProAla: 7.28 ± 0.095
0.158ProCys: 0.158 ± 0.014
4.121ProAsp: 4.121 ± 0.068
3.793ProGlu: 3.793 ± 0.066
1.768ProPhe: 1.768 ± 0.039
5.061ProGly: 5.061 ± 0.08
1.142ProHis: 1.142 ± 0.034
2.159ProIle: 2.159 ± 0.045
0.847ProLys: 0.847 ± 0.031
4.998ProLeu: 4.998 ± 0.084
0.836ProMet: 0.836 ± 0.029
0.99ProAsn: 0.99 ± 0.03
2.553ProPro: 2.553 ± 0.064
1.453ProGln: 1.453 ± 0.037
3.628ProArg: 3.628 ± 0.071
3.368ProSer: 3.368 ± 0.055
3.545ProThr: 3.545 ± 0.063
4.998ProVal: 4.998 ± 0.065
0.891ProTrp: 0.891 ± 0.036
0.994ProTyr: 0.994 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.132GlnAla: 3.132 ± 0.062
0.093GlnCys: 0.093 ± 0.008
1.205GlnAsp: 1.205 ± 0.035
1.21GlnGlu: 1.21 ± 0.039
0.843GlnPhe: 0.843 ± 0.025
1.957GlnGly: 1.957 ± 0.048
0.589GlnHis: 0.589 ± 0.024
1.306GlnIle: 1.306 ± 0.032
0.577GlnLys: 0.577 ± 0.024
2.857GlnLeu: 2.857 ± 0.054
0.452GlnMet: 0.452 ± 0.021
0.648GlnAsn: 0.648 ± 0.025
1.406GlnPro: 1.406 ± 0.036
0.979GlnGln: 0.979 ± 0.033
2.232GlnArg: 2.232 ± 0.052
1.482GlnSer: 1.482 ± 0.041
1.576GlnThr: 1.576 ± 0.04
2.063GlnVal: 2.063 ± 0.045
0.441GlnTrp: 0.441 ± 0.021
0.568GlnTyr: 0.568 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
9.178ArgAla: 9.178 ± 0.119
0.332ArgCys: 0.332 ± 0.018
4.601ArgAsp: 4.601 ± 0.089
4.356ArgGlu: 4.356 ± 0.091
2.404ArgPhe: 2.404 ± 0.049
5.75ArgGly: 5.75 ± 0.088
1.58ArgHis: 1.58 ± 0.044
3.759ArgIle: 3.759 ± 0.061
1.205ArgLys: 1.205 ± 0.034
7.204ArgLeu: 7.204 ± 0.121
1.655ArgMet: 1.655 ± 0.037
1.303ArgAsn: 1.303 ± 0.034
3.656ArgPro: 3.656 ± 0.078
1.859ArgGln: 1.859 ± 0.038
6.711ArgArg: 6.711 ± 0.119
4.231ArgSer: 4.231 ± 0.068
4.403ArgThr: 4.403 ± 0.074
6.346ArgVal: 6.346 ± 0.086
1.158ArgTrp: 1.158 ± 0.037
1.509ArgTyr: 1.509 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
7.64SerAla: 7.64 ± 0.131
0.256SerCys: 0.256 ± 0.015
3.624SerAsp: 3.624 ± 0.063
2.85SerGlu: 2.85 ± 0.053
1.977SerPhe: 1.977 ± 0.041
5.943SerGly: 5.943 ± 0.094
1.035SerHis: 1.035 ± 0.033
2.73SerIle: 2.73 ± 0.053
0.939SerLys: 0.939 ± 0.031
5.542SerLeu: 5.542 ± 0.068
1.135SerMet: 1.135 ± 0.033
1.057SerAsn: 1.057 ± 0.033
3.355SerPro: 3.355 ± 0.058
1.405SerGln: 1.405 ± 0.04
4.041SerArg: 4.041 ± 0.073
3.605SerSer: 3.605 ± 0.082
3.921SerThr: 3.921 ± 0.095
5.074SerVal: 5.074 ± 0.087
0.974SerTrp: 0.974 ± 0.033
1.161SerTyr: 1.161 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
8.01ThrAla: 8.01 ± 0.166
0.281ThrCys: 0.281 ± 0.016
3.929ThrAsp: 3.929 ± 0.067
3.021ThrGlu: 3.021 ± 0.054
1.993ThrPhe: 1.993 ± 0.048
5.77ThrGly: 5.77 ± 0.103
1.152ThrHis: 1.152 ± 0.034
3.164ThrIle: 3.164 ± 0.078
1.085ThrLys: 1.085 ± 0.037
6.223ThrLeu: 6.223 ± 0.107
0.974ThrMet: 0.974 ± 0.03
1.285ThrAsn: 1.285 ± 0.036
4.138ThrPro: 4.138 ± 0.074
1.492ThrGln: 1.492 ± 0.039
4.057ThrArg: 4.057 ± 0.061
3.81ThrSer: 3.81 ± 0.088
4.235ThrThr: 4.235 ± 0.117
5.902ThrVal: 5.902 ± 0.113
0.929ThrTrp: 0.929 ± 0.031
1.347ThrTyr: 1.347 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
11.971ValAla: 11.971 ± 0.144
0.512ValCys: 0.512 ± 0.022
6.095ValAsp: 6.095 ± 0.079
5.08ValGlu: 5.08 ± 0.079
2.964ValPhe: 2.964 ± 0.06
7.294ValGly: 7.294 ± 0.09
1.628ValHis: 1.628 ± 0.036
4.318ValIle: 4.318 ± 0.07
1.505ValLys: 1.505 ± 0.043
9.149ValLeu: 9.149 ± 0.123
1.406ValMet: 1.406 ± 0.041
1.79ValAsn: 1.79 ± 0.055
4.845ValPro: 4.845 ± 0.072
2.229ValGln: 2.229 ± 0.042
6.432ValArg: 6.432 ± 0.087
5.415ValSer: 5.415 ± 0.082
6.259ValThr: 6.259 ± 0.143
9.436ValVal: 9.436 ± 0.125
1.288ValTrp: 1.288 ± 0.037
1.65ValTyr: 1.65 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
1.646TrpAla: 1.646 ± 0.038
0.103TrpCys: 0.103 ± 0.01
0.809TrpAsp: 0.809 ± 0.026
0.685TrpGlu: 0.685 ± 0.027
0.561TrpPhe: 0.561 ± 0.024
1.195TrpGly: 1.195 ± 0.036
0.337TrpHis: 0.337 ± 0.019
0.778TrpIle: 0.778 ± 0.031
0.295TrpLys: 0.295 ± 0.019
1.657TrpLeu: 1.657 ± 0.045
0.38TrpMet: 0.38 ± 0.018
0.457TrpAsn: 0.457 ± 0.021
0.752TrpPro: 0.752 ± 0.029
0.55TrpGln: 0.55 ± 0.025
1.292TrpArg: 1.292 ± 0.042
1.048TrpSer: 1.048 ± 0.037
1.001TrpThr: 1.001 ± 0.032
1.255TrpVal: 1.255 ± 0.035
0.362TrpTrp: 0.362 ± 0.022
0.319TrpTyr: 0.319 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.479TyrAla: 2.479 ± 0.048
0.118TyrCys: 0.118 ± 0.01
1.403TyrAsp: 1.403 ± 0.035
1.141TyrGlu: 1.141 ± 0.036
0.683TyrPhe: 0.683 ± 0.028
1.896TyrGly: 1.896 ± 0.044
0.29TyrHis: 0.29 ± 0.018
0.743TyrIle: 0.743 ± 0.025
0.304TyrLys: 0.304 ± 0.017
2.026TyrLeu: 2.026 ± 0.051
0.264TyrMet: 0.264 ± 0.014
0.475TyrAsn: 0.475 ± 0.024
1.012TyrPro: 1.012 ± 0.035
0.535TyrGln: 0.535 ± 0.021
1.619TyrArg: 1.619 ± 0.042
1.079TyrSer: 1.079 ± 0.032
1.146TyrThr: 1.146 ± 0.037
1.666TyrVal: 1.666 ± 0.046
0.341TyrTrp: 0.341 ± 0.018
0.459TyrTyr: 0.459 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3245 proteins (1108321 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski