Phaeobacter gallaeciensis
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3897 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B0ZND8|A0A1B0ZND8_9RHOB Bcr/CflA family efflux transporter OS=Phaeobacter gallaeciensis OX=60890 GN=bcr PE=3 SV=1
MM1 pKa = 7.28 NKK3 pKa = 8.0 ITGAFIAALALSACGNGNPFTEE25 pKa = 4.52 DD26 pKa = 2.98 TTDD29 pKa = 3.15 NGGDD33 pKa = 3.37 TGTDD37 pKa = 2.91 TGTDD41 pKa = 3.16 TGIDD45 pKa = 3.63 GDD47 pKa = 4.28 GRR49 pKa = 11.84 PPGTPSPTPDD59 pKa = 2.76 TSIFRR64 pKa = 11.84 RR65 pKa = 11.84 EE66 pKa = 4.04 GTSTEE71 pKa = 3.83 DD72 pKa = 3.18 AYY74 pKa = 10.18 IGNGYY79 pKa = 8.13 ATEE82 pKa = 3.93 ISYY85 pKa = 11.15 DD86 pKa = 3.77 SNNDD90 pKa = 3.06 TFSVDD95 pKa = 3.11 NLAFDD100 pKa = 4.71 GDD102 pKa = 3.69 GTYY105 pKa = 11.19 ARR107 pKa = 11.84 GTAVSSLGPFAVYY120 pKa = 9.77 EE121 pKa = 4.09 ADD123 pKa = 3.42 AQYY126 pKa = 11.66 SDD128 pKa = 3.31 ITDD131 pKa = 3.61 NEE133 pKa = 4.75 PINQFTHH140 pKa = 6.18 RR141 pKa = 11.84 AIYY144 pKa = 9.89 GVSTSGNSKK153 pKa = 9.89 FAIVRR158 pKa = 11.84 TGAYY162 pKa = 9.9 ADD164 pKa = 3.58 YY165 pKa = 10.8 GFGGFVYY172 pKa = 10.54 QRR174 pKa = 11.84 DD175 pKa = 3.71 NSVTLPSSGQAQYY188 pKa = 11.12 NGSLAGLRR196 pKa = 11.84 DD197 pKa = 3.75 YY198 pKa = 11.59 NGAAGLEE205 pKa = 4.4 YY206 pKa = 8.59 TTADD210 pKa = 3.33 VQIAIDD216 pKa = 4.21 FDD218 pKa = 4.34 DD219 pKa = 5.04 FNPATGGRR227 pKa = 11.84 GDD229 pKa = 3.67 AVRR232 pKa = 11.84 GSLTNRR238 pKa = 11.84 TIYY241 pKa = 9.46 DD242 pKa = 3.48 TAGNDD247 pKa = 3.0 ITNDD251 pKa = 3.17 VINRR255 pKa = 11.84 INVDD259 pKa = 3.24 NNLTLSSIPVAVFDD273 pKa = 4.62 VGPNNLDD280 pKa = 3.65 DD281 pKa = 4.16 NGEE284 pKa = 4.33 IIGTLQSTYY293 pKa = 10.87 VDD295 pKa = 3.6 ANGTAQTYY303 pKa = 7.3 EE304 pKa = 3.9 TGNYY308 pKa = 8.01 YY309 pKa = 10.9 AVVSGDD315 pKa = 3.32 NAEE318 pKa = 4.49 EE319 pKa = 3.94 IVGVVVLEE327 pKa = 4.28 NSLDD331 pKa = 3.58 PAANSVRR338 pKa = 11.84 EE339 pKa = 3.96 TGGFIVYY346 pKa = 10.64 NNN348 pKa = 3.49
Molecular weight: 36.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.465
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.821
Patrickios 0.629
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A1B0ZP72|A0A1B0ZP72_9RHOB LysR family transcriptional regulator OS=Phaeobacter gallaeciensis OX=60890 GN=JL2886_00986 PE=3 SV=1
MM1 pKa = 7.34 TEE3 pKa = 3.71 QVVIVSPSGVARR15 pKa = 11.84 LQRR18 pKa = 11.84 EE19 pKa = 4.35 AEE21 pKa = 4.13 RR22 pKa = 11.84 IAAIKK27 pKa = 10.5 ARR29 pKa = 11.84 GAVPRR34 pKa = 11.84 QCGDD38 pKa = 4.81 AIPEE42 pKa = 4.15 APARR46 pKa = 11.84 GAFQVFEE53 pKa = 4.34 PMRR56 pKa = 11.84 LCPVGADD63 pKa = 3.83 DD64 pKa = 6.35 YY65 pKa = 10.7 EE66 pKa = 4.39 AQPVGYY72 pKa = 9.25 RR73 pKa = 11.84 GRR75 pKa = 11.84 SAIRR79 pKa = 11.84 NADD82 pKa = 3.42 VFDD85 pKa = 3.96 VMAAAAARR93 pKa = 11.84 KK94 pKa = 8.96 KK95 pKa = 10.57 KK96 pKa = 9.59 PAPFTLGQIAMGRR109 pKa = 11.84 HH110 pKa = 4.6 YY111 pKa = 10.69 RR112 pKa = 11.84 DD113 pKa = 2.88 LVEE116 pKa = 4.3 RR117 pKa = 11.84 HH118 pKa = 4.79 ATAGVRR124 pKa = 11.84 CSSVEE129 pKa = 3.74 ALRR132 pKa = 11.84 TGGSGQGGEE141 pKa = 4.81 FIDD144 pKa = 4.16 AVLRR148 pKa = 11.84 DD149 pKa = 3.78 RR150 pKa = 11.84 DD151 pKa = 4.12 EE152 pKa = 5.09 IDD154 pKa = 3.02 LLRR157 pKa = 11.84 RR158 pKa = 11.84 RR159 pKa = 11.84 IGTGSAIEE167 pKa = 4.06 VRR169 pKa = 11.84 RR170 pKa = 11.84 VRR172 pKa = 11.84 PSKK175 pKa = 10.12 RR176 pKa = 11.84 GSRR179 pKa = 11.84 VSITNRR185 pKa = 11.84 RR186 pKa = 11.84 LVDD189 pKa = 3.38 MVCIEE194 pKa = 4.17 DD195 pKa = 3.44 RR196 pKa = 11.84 SLRR199 pKa = 11.84 EE200 pKa = 3.61 VLEE203 pKa = 3.83 AHH205 pKa = 6.36 GWSVYY210 pKa = 10.88 GEE212 pKa = 4.15 TVKK215 pKa = 10.76 AVRR218 pKa = 11.84 AALASVLDD226 pKa = 4.22 RR227 pKa = 11.84 MAGPVRR233 pKa = 11.84 TGRR236 pKa = 11.84 IHH238 pKa = 6.27 TLHH241 pKa = 6.87 KK242 pKa = 10.63 GAA244 pKa = 5.08
Molecular weight: 26.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.37
IPC_protein 10.218
Toseland 10.467
ProMoST 10.218
Dawson 10.57
Bjellqvist 10.321
Wikipedia 10.804
Rodwell 10.628
Grimsley 10.628
Solomon 10.716
Lehninger 10.672
Nozaki 10.482
DTASelect 10.306
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.511
Patrickios 10.335
IPC_peptide 10.716
IPC2_peptide 9.516
IPC2.peptide.svr19 8.58
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3897
0
3897
1197673
37
2173
307.3
33.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.156 ± 0.048
0.915 ± 0.013
6.016 ± 0.037
6.147 ± 0.036
3.659 ± 0.023
8.705 ± 0.039
2.06 ± 0.022
5.096 ± 0.032
3.351 ± 0.032
10.123 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.891 ± 0.019
2.624 ± 0.024
5.012 ± 0.031
3.485 ± 0.024
6.394 ± 0.039
5.344 ± 0.029
5.386 ± 0.029
7.068 ± 0.035
1.346 ± 0.016
2.223 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here