Klebsiella phage vB_KpnS_FZ41

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Sugarlandvirus; unclassified Sugarlandvirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T530|A0A4D6T530_9CAUD Anaerobic ribonucleoside-triphosphate reductase OS=Klebsiella phage vB_KpnS_FZ41 OX=2530030 GN=FZ41_53 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 5.61FIVVCGANTDD12 pKa = 3.91CFEE15 pKa = 4.96LLNDD19 pKa = 4.48ALDD22 pKa = 3.97KK23 pKa = 11.13VDD25 pKa = 3.82EE26 pKa = 4.53HH27 pKa = 5.78MQEE30 pKa = 3.78GRR32 pKa = 11.84TPTFIDD38 pKa = 4.11LSQGKK43 pKa = 7.51TYY45 pKa = 9.83FYY47 pKa = 10.41PSLDD51 pKa = 3.4VEE53 pKa = 4.52PTVLPIFMHH62 pKa = 5.69SLSWDD67 pKa = 3.68EE68 pKa = 4.83EE69 pKa = 4.34DD70 pKa = 5.24DD71 pKa = 3.74

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T568|A0A4D6T568_9CAUD L-alanyl-D-glutamate peptidase OS=Klebsiella phage vB_KpnS_FZ41 OX=2530030 GN=FZ41_6 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84TLRR5 pKa = 11.84GGSPKK10 pKa = 10.14SRR12 pKa = 11.84SHH14 pKa = 6.68NVYY17 pKa = 9.32QLNVIRR23 pKa = 11.84DD24 pKa = 3.89GQKK27 pKa = 9.79KK28 pKa = 10.33GGGGGEE34 pKa = 3.56WGFRR38 pKa = 11.84VIMVIIMTLIFLQSCQQ54 pKa = 3.3

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

23108

39

1485

296.3

33.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.283 ± 0.514

0.965 ± 0.099

6.175 ± 0.202

6.539 ± 0.248

4.085 ± 0.167

6.915 ± 0.275

1.839 ± 0.166

6.249 ± 0.182

6.552 ± 0.268

8.114 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.67 ± 0.178

5.466 ± 0.24

3.804 ± 0.203

3.466 ± 0.2

4.782 ± 0.152

6.699 ± 0.23

5.829 ± 0.267

6.656 ± 0.192

1.272 ± 0.14

3.639 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski