Aspergillus leporis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae;

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12743 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N5WSY2|A0A5N5WSY2_9EURO S-adenosyl-L-methionine-dependent methyltransferase OS=Aspergillus leporis OX=41062 GN=BDV29DRAFT_197223 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 9.96PSTFLSSLALIGPTALTAAQTLNIVAHH29 pKa = 6.25QDD31 pKa = 3.29DD32 pKa = 6.1DD33 pKa = 3.87ILFISPEE40 pKa = 3.72ILSDD44 pKa = 3.61VRR46 pKa = 11.84SGRR49 pKa = 11.84AVRR52 pKa = 11.84TIFLTAGDD60 pKa = 4.18AGNGEE65 pKa = 4.84AYY67 pKa = 6.59WTSRR71 pKa = 11.84QAGSLAAYY79 pKa = 10.28AEE81 pKa = 4.13ILGVSNEE88 pKa = 3.9WDD90 pKa = 3.31EE91 pKa = 4.31GDD93 pKa = 3.84AGIEE97 pKa = 4.13GFDD100 pKa = 3.28IPVYY104 pKa = 10.57SLSAQPQVEE113 pKa = 4.37LAFLHH118 pKa = 6.8LPDD121 pKa = 5.32GNMDD125 pKa = 3.6GQGFPATGGVSLRR138 pKa = 11.84QLWEE142 pKa = 3.8GDD144 pKa = 3.34IEE146 pKa = 4.74SLGTVDD152 pKa = 5.9ASGTTYY158 pKa = 10.72TKK160 pKa = 11.06DD161 pKa = 3.23EE162 pKa = 5.39LIDD165 pKa = 3.61VLADD169 pKa = 3.45IIEE172 pKa = 4.35NFEE175 pKa = 4.15PDD177 pKa = 4.0RR178 pKa = 11.84INTLDD183 pKa = 3.58YY184 pKa = 11.65VNDD187 pKa = 3.86IGDD190 pKa = 4.15GDD192 pKa = 4.26HH193 pKa = 7.15SDD195 pKa = 4.1HH196 pKa = 6.18YY197 pKa = 8.08TTGYY201 pKa = 8.16FTDD204 pKa = 4.01HH205 pKa = 7.27ASQEE209 pKa = 4.08ADD211 pKa = 2.92NDD213 pKa = 3.93ADD215 pKa = 3.84LVGYY219 pKa = 8.69MGYY222 pKa = 10.7GINNLPVNLDD232 pKa = 3.35ANTIADD238 pKa = 4.29KK239 pKa = 11.41KK240 pKa = 10.79NIFYY244 pKa = 9.76TYY246 pKa = 10.15AAHH249 pKa = 8.11DD250 pKa = 3.95SATCQNDD257 pKa = 3.38AACASRR263 pKa = 11.84PEE265 pKa = 4.23INWLQRR271 pKa = 11.84QYY273 pKa = 10.84TVV275 pKa = 2.86

Molecular weight: 29.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N5WKX7|A0A5N5WKX7_9EURO Amine oxidase OS=Aspergillus leporis OX=41062 GN=BDV29DRAFT_199969 PE=3 SV=1
MM1 pKa = 7.64LLLLDD6 pKa = 4.33LQRR9 pKa = 11.84QNPHH13 pKa = 5.57QPPTKK18 pKa = 9.13TRR20 pKa = 11.84KK21 pKa = 9.85LNGTAAHH28 pKa = 6.6FSPFRR33 pKa = 11.84TLSRR37 pKa = 11.84SLCLFFFSPALSSFYY52 pKa = 10.95YY53 pKa = 10.52SVFPLPTQTPPPSRR67 pKa = 11.84PNLSPLRR74 pKa = 11.84PIRR77 pKa = 11.84HH78 pKa = 5.6PFIRR82 pKa = 11.84CLWMRR87 pKa = 11.84ASSDD91 pKa = 3.58FHH93 pKa = 7.23SLPIQFLFSLNLTTQEE109 pKa = 3.5KK110 pKa = 10.77SIIKK114 pKa = 9.77KK115 pKa = 8.87RR116 pKa = 11.84QHH118 pKa = 5.8FKK120 pKa = 10.72KK121 pKa = 9.2EE122 pKa = 3.64KK123 pKa = 7.83PGIYY127 pKa = 10.03FFFSFFFSHH136 pKa = 7.65LILFSNFLRR145 pKa = 11.84SCFFFFSLLPFHH157 pKa = 7.01RR158 pKa = 11.84SIYY161 pKa = 10.55RR162 pKa = 11.84LVCLNKK168 pKa = 10.21GSVPGRR174 pKa = 11.84GPEE177 pKa = 4.17SVNPLGGCTEE187 pKa = 3.74ILRR190 pKa = 11.84RR191 pKa = 11.84IRR193 pKa = 11.84FSWVLRR199 pKa = 11.84LLYY202 pKa = 10.54APSLLHH208 pKa = 6.42LSCMICCSILLFFPPPPDD226 pKa = 3.11

Molecular weight: 26.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12743

0

12743

5742925

49

6910

450.7

50.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.199 ± 0.018

1.341 ± 0.008

5.488 ± 0.014

5.996 ± 0.02

3.877 ± 0.013

6.808 ± 0.02

2.459 ± 0.009

5.153 ± 0.014

4.633 ± 0.02

9.246 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.007

3.731 ± 0.01

5.877 ± 0.021

3.987 ± 0.014

6.04 ± 0.018

8.354 ± 0.022

5.943 ± 0.011

6.242 ± 0.016

1.51 ± 0.008

2.942 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski