Synechococcus sp. (strain WH7803)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2530 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5GK03|A5GK03_SYNPW Uncharacterized protein OS=Synechococcus sp. (strain WH7803) OX=32051 GN=SynWH7803_0842 PE=4 SV=1
MM1 pKa = 7.31LRR3 pKa = 11.84SLLSLLFAAVMWVQVPQWQNDD24 pKa = 3.2WSQCAVDD31 pKa = 4.88VPDD34 pKa = 5.33VNCHH38 pKa = 5.43WYY40 pKa = 8.05ITAPDD45 pKa = 3.55NTFGEE50 pKa = 4.69GFDD53 pKa = 3.44WATAPWFDD61 pKa = 4.07VNGLSDD67 pKa = 3.78VAEE70 pKa = 4.22LTNTVSNLQHH80 pKa = 6.44

Molecular weight: 9.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5GN11|A5GN11_SYNPW bPH_2 domain-containing protein OS=Synechococcus sp. (strain WH7803) OX=32051 GN=SynWH7803_1900 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.01RR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 9.07RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92RR38 pKa = 11.84GRR40 pKa = 11.84SRR42 pKa = 11.84LAVV45 pKa = 3.35

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2530

0

2530

731277

31

2208

289.0

31.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.627 ± 0.059

1.226 ± 0.019

5.443 ± 0.054

5.621 ± 0.046

3.087 ± 0.037

8.3 ± 0.048

2.107 ± 0.03

4.221 ± 0.045

2.507 ± 0.037

12.59 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.048 ± 0.023

2.623 ± 0.038

5.558 ± 0.043

4.741 ± 0.041

7.282 ± 0.057

6.495 ± 0.05

4.867 ± 0.036

7.103 ± 0.047

1.761 ± 0.029

1.794 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski