Amino acid dipepetide frequency for Synechococcus sp. (strain WH7803)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.899AlaAla: 12.899 ± 0.214	1.5AlaCys: 1.5 ± 0.048	6.128AlaAsp: 6.128 ± 0.11	6.199AlaGlu: 6.199 ± 0.104	3.313AlaPhe: 3.313 ± 0.071	8.574AlaGly: 8.574 ± 0.132	1.823AlaHis: 1.823 ± 0.052	4.995AlaIle: 4.995 ± 0.091	2.52AlaLys: 2.52 ± 0.075	14.241AlaLeu: 14.241 ± 0.209	2.921AlaMet: 2.921 ± 0.073	2.37AlaAsn: 2.37 ± 0.062	4.919AlaPro: 4.919 ± 0.098	4.071AlaGln: 4.071 ± 0.071	6.322AlaArg: 6.322 ± 0.101	7.062AlaSer: 7.062 ± 0.095	4.606AlaThr: 4.606 ± 0.074	8.209AlaVal: 8.209 ± 0.121	1.681AlaTrp: 1.681 ± 0.054	1.916AlaTyr: 1.916 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	1.056CysAla: 1.056 ± 0.043	0.269CysCys: 0.269 ± 0.019	0.706CysAsp: 0.706 ± 0.034	0.633CysGlu: 0.633 ± 0.028	0.455CysPhe: 0.455 ± 0.028	1.181CysGly: 1.181 ± 0.047	0.342CysHis: 0.342 ± 0.023	0.459CysIle: 0.459 ± 0.024	0.268CysLys: 0.268 ± 0.02	1.432CysLeu: 1.432 ± 0.052	0.189CysMet: 0.189 ± 0.018	0.33CysAsn: 0.33 ± 0.026	0.741CysPro: 0.741 ± 0.035	0.409CysGln: 0.409 ± 0.023	1.132CysArg: 1.132 ± 0.039	0.892CysSer: 0.892 ± 0.031	0.532CysThr: 0.532 ± 0.026	0.703CysVal: 0.703 ± 0.031	0.32CysTrp: 0.32 ± 0.022	0.213CysTyr: 0.213 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.981AspAla: 5.981 ± 0.11	0.625AspCys: 0.625 ± 0.033	2.987AspAsp: 2.987 ± 0.091	2.702AspGlu: 2.702 ± 0.07	1.608AspPhe: 1.608 ± 0.053	5.001AspGly: 5.001 ± 0.104	1.346AspHis: 1.346 ± 0.056	1.504AspIle: 1.504 ± 0.047	1.0AspLys: 1.0 ± 0.044	7.535AspLeu: 7.535 ± 0.109	0.762AspMet: 0.762 ± 0.034	1.194AspAsn: 1.194 ± 0.06	4.089AspPro: 4.089 ± 0.078	3.174AspGln: 3.174 ± 0.075	4.18AspArg: 4.18 ± 0.077	2.836AspSer: 2.836 ± 0.111	2.122AspThr: 2.122 ± 0.065	3.595AspVal: 3.595 ± 0.101	1.061AspTrp: 1.061 ± 0.041	1.127AspTyr: 1.127 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	7.259GluAla: 7.259 ± 0.112	0.461GluCys: 0.461 ± 0.03	2.42GluAsp: 2.42 ± 0.065	3.168GluGlu: 3.168 ± 0.084	1.228GluPhe: 1.228 ± 0.042	4.599GluGly: 4.599 ± 0.081	1.257GluHis: 1.257 ± 0.048	2.28GluIle: 2.28 ± 0.065	1.408GluLys: 1.408 ± 0.05	7.142GluLeu: 7.142 ± 0.112	1.034GluMet: 1.034 ± 0.041	1.151GluAsn: 1.151 ± 0.042	2.999GluPro: 2.999 ± 0.069	3.509GluGln: 3.509 ± 0.084	5.426GluArg: 5.426 ± 0.081	3.214GluSer: 3.214 ± 0.087	2.653GluThr: 2.653 ± 0.06	3.659GluVal: 3.659 ± 0.08	0.702GluTrp: 0.702 ± 0.031	0.639GluTyr: 0.639 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	2.966PheAla: 2.966 ± 0.072	0.487PheCys: 0.487 ± 0.025	2.006PheAsp: 2.006 ± 0.052	1.76PheGlu: 1.76 ± 0.05	1.06PhePhe: 1.06 ± 0.043	2.902PheGly: 2.902 ± 0.074	0.676PheHis: 0.676 ± 0.036	1.231PheIle: 1.231 ± 0.042	0.811PheLys: 0.811 ± 0.032	3.157PheLeu: 3.157 ± 0.083	0.573PheMet: 0.573 ± 0.028	1.112PheAsn: 1.112 ± 0.046	1.419PhePro: 1.419 ± 0.047	1.376PheGln: 1.376 ± 0.041	2.025PheArg: 2.025 ± 0.051	2.427PheSer: 2.427 ± 0.08	1.694PheThr: 1.694 ± 0.052	1.895PheVal: 1.895 ± 0.063	0.57PheTrp: 0.57 ± 0.033	0.726PheTyr: 0.726 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	7.44GlyAla: 7.44 ± 0.117	1.281GlyCys: 1.281 ± 0.045	4.711GlyAsp: 4.711 ± 0.105	4.435GlyGlu: 4.435 ± 0.083	3.274GlyPhe: 3.274 ± 0.066	7.103GlyGly: 7.103 ± 0.163	1.802GlyHis: 1.802 ± 0.049	3.813GlyIle: 3.813 ± 0.093	2.189GlyLys: 2.189 ± 0.064	10.726GlyLeu: 10.726 ± 0.122	1.869GlyMet: 1.869 ± 0.065	2.073GlyAsn: 2.073 ± 0.088	3.349GlyPro: 3.349 ± 0.069	3.506GlyGln: 3.506 ± 0.067	5.78GlyArg: 5.78 ± 0.111	5.619GlySer: 5.619 ± 0.104	4.187GlyThr: 4.187 ± 0.09	6.185GlyVal: 6.185 ± 0.103	1.823GlyTrp: 1.823 ± 0.056	1.834GlyTyr: 1.834 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	1.802HisAla: 1.802 ± 0.055	0.421HisCys: 0.421 ± 0.027	1.076HisAsp: 1.076 ± 0.046	0.877HisGlu: 0.877 ± 0.033	0.678HisPhe: 0.678 ± 0.03	1.991HisGly: 1.991 ± 0.061	0.841HisHis: 0.841 ± 0.046	0.633HisIle: 0.633 ± 0.033	0.364HisLys: 0.364 ± 0.022	2.621HisLeu: 2.621 ± 0.068	0.257HisMet: 0.257 ± 0.019	0.531HisAsn: 0.531 ± 0.028	1.726HisPro: 1.726 ± 0.049	1.153HisGln: 1.153 ± 0.044	1.86HisArg: 1.86 ± 0.061	1.164HisSer: 1.164 ± 0.042	0.833HisThr: 0.833 ± 0.033	1.091HisVal: 1.091 ± 0.044	0.677HisTrp: 0.677 ± 0.031	0.476HisTyr: 0.476 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	4.881IleAla: 4.881 ± 0.096	0.518IleCys: 0.518 ± 0.027	2.792IleAsp: 2.792 ± 0.067	2.712IleGlu: 2.712 ± 0.074	1.028IlePhe: 1.028 ± 0.05	4.119IleGly: 4.119 ± 0.096	1.016IleHis: 1.016 ± 0.04	1.201IleIle: 1.201 ± 0.052	1.231IleLys: 1.231 ± 0.045	3.535IleLeu: 3.535 ± 0.079	0.383IleMet: 0.383 ± 0.026	1.566IleAsn: 1.566 ± 0.056	2.608IlePro: 2.608 ± 0.055	1.864IleGln: 1.864 ± 0.056	2.924IleArg: 2.924 ± 0.07	2.961IleSer: 2.961 ± 0.076	2.78IleThr: 2.78 ± 0.073	2.632IleVal: 2.632 ± 0.077	0.656IleTrp: 0.656 ± 0.032	0.805IleTyr: 0.805 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	3.255LysAla: 3.255 ± 0.075	0.15LysCys: 0.15 ± 0.014	1.411LysAsp: 1.411 ± 0.049	1.499LysGlu: 1.499 ± 0.053	0.51LysPhe: 0.51 ± 0.026	2.135LysGly: 2.135 ± 0.066	0.421LysHis: 0.421 ± 0.026	0.952LysIle: 0.952 ± 0.041	0.844LysLys: 0.844 ± 0.044	2.645LysLeu: 2.645 ± 0.072	0.362LysMet: 0.362 ± 0.024	0.639LysAsn: 0.639 ± 0.035	1.718LysPro: 1.718 ± 0.056	1.302LysGln: 1.302 ± 0.041	2.021LysArg: 2.021 ± 0.058	1.396LysSer: 1.396 ± 0.058	1.441LysThr: 1.441 ± 0.05	1.831LysVal: 1.831 ± 0.056	0.156LysTrp: 0.156 ± 0.013	0.386LysTyr: 0.386 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.27LeuAla: 13.27 ± 0.192	1.357LeuCys: 1.357 ± 0.043	6.583LeuAsp: 6.583 ± 0.118	8.121LeuGlu: 8.121 ± 0.122	3.521LeuPhe: 3.521 ± 0.08	9.492LeuGly: 9.492 ± 0.132	2.523LeuHis: 2.523 ± 0.067	5.942LeuIle: 5.942 ± 0.116	3.668LeuLys: 3.668 ± 0.078	15.813LeuLeu: 15.813 ± 0.234	2.847LeuMet: 2.847 ± 0.07	3.64LeuAsn: 3.64 ± 0.082	7.067LeuPro: 7.067 ± 0.123	6.575LeuGln: 6.575 ± 0.14	8.846LeuArg: 8.846 ± 0.146	8.19LeuSer: 8.19 ± 0.127	5.882LeuThr: 5.882 ± 0.087	8.931LeuVal: 8.931 ± 0.145	1.817LeuTrp: 1.817 ± 0.062	1.816LeuTyr: 1.816 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	3.037MetAla: 3.037 ± 0.063	0.114MetCys: 0.114 ± 0.014	1.045MetAsp: 1.045 ± 0.042	1.121MetGlu: 1.121 ± 0.041	0.388MetPhe: 0.388 ± 0.021	1.757MetGly: 1.757 ± 0.05	0.398MetHis: 0.398 ± 0.024	0.825MetIle: 0.825 ± 0.039	0.585MetLys: 0.585 ± 0.03	2.132MetLeu: 2.132 ± 0.053	0.286MetMet: 0.286 ± 0.017	0.555MetAsn: 0.555 ± 0.025	1.372MetPro: 1.372 ± 0.047	0.885MetGln: 0.885 ± 0.038	1.358MetArg: 1.358 ± 0.042	1.268MetSer: 1.268 ± 0.044	1.384MetThr: 1.384 ± 0.041	1.652MetVal: 1.652 ± 0.05	0.122MetTrp: 0.122 ± 0.014	0.194MetTyr: 0.194 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.792AsnAla: 2.792 ± 0.064	0.283AsnCys: 0.283 ± 0.02	1.603AsnAsp: 1.603 ± 0.073	1.127AsnGlu: 1.127 ± 0.041	0.836AsnPhe: 0.836 ± 0.04	2.401AsnGly: 2.401 ± 0.063	0.578AsnHis: 0.578 ± 0.029	0.879AsnIle: 0.879 ± 0.046	0.65AsnLys: 0.65 ± 0.035	3.092AsnLeu: 3.092 ± 0.073	0.334AsnMet: 0.334 ± 0.021	0.841AsnAsn: 0.841 ± 0.046	1.947AsnPro: 1.947 ± 0.055	1.277AsnGln: 1.277 ± 0.05	2.072AsnArg: 2.072 ± 0.052	1.573AsnSer: 1.573 ± 0.062	1.355AsnThr: 1.355 ± 0.05	1.559AsnVal: 1.559 ± 0.058	0.496AsnTrp: 0.496 ± 0.028	0.533AsnTyr: 0.533 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	5.832ProAla: 5.832 ± 0.1	0.602ProCys: 0.602 ± 0.029	3.421ProAsp: 3.421 ± 0.072	4.07ProGlu: 4.07 ± 0.075	1.797ProPhe: 1.797 ± 0.061	4.664ProGly: 4.664 ± 0.083	1.001ProHis: 1.001 ± 0.036	2.207ProIle: 2.207 ± 0.063	1.283ProLys: 1.283 ± 0.056	7.064ProLeu: 7.064 ± 0.123	1.274ProMet: 1.274 ± 0.044	1.265ProAsn: 1.265 ± 0.041	2.786ProPro: 2.786 ± 0.085	2.392ProGln: 2.392 ± 0.054	2.958ProArg: 2.958 ± 0.073	3.895ProSer: 3.895 ± 0.078	2.616ProThr: 2.616 ± 0.05	4.286ProVal: 4.286 ± 0.086	1.127ProTrp: 1.127 ± 0.044	1.038ProTyr: 1.038 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	5.648GlnAla: 5.648 ± 0.107	0.432GlnCys: 0.432 ± 0.028	1.949GlnAsp: 1.949 ± 0.045	2.548GlnGlu: 2.548 ± 0.063	1.213GlnPhe: 1.213 ± 0.046	3.633GlnGly: 3.633 ± 0.067	0.983GlnHis: 0.983 ± 0.037	1.808GlnIle: 1.808 ± 0.056	1.105GlnLys: 1.105 ± 0.043	6.249GlnLeu: 6.249 ± 0.115	0.831GlnMet: 0.831 ± 0.034	0.968GlnAsn: 0.968 ± 0.035	2.857GlnPro: 2.857 ± 0.062	3.1GlnGln: 3.1 ± 0.089	4.722GlnArg: 4.722 ± 0.088	2.678GlnSer: 2.678 ± 0.064	2.033GlnThr: 2.033 ± 0.049	3.231GlnVal: 3.231 ± 0.074	0.926GlnTrp: 0.926 ± 0.041	0.492GlnTyr: 0.492 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	5.649ArgAla: 5.649 ± 0.098	1.18ArgCys: 1.18 ± 0.044	3.791ArgAsp: 3.791 ± 0.089	3.825ArgGlu: 3.825 ± 0.082	2.829ArgPhe: 2.829 ± 0.065	4.816ArgGly: 4.816 ± 0.098	1.783ArgHis: 1.783 ± 0.06	3.266ArgIle: 3.266 ± 0.064	1.641ArgLys: 1.641 ± 0.053	9.936ArgLeu: 9.936 ± 0.153	1.631ArgMet: 1.631 ± 0.058	1.842ArgAsn: 1.842 ± 0.042	3.776ArgPro: 3.776 ± 0.094	4.03ArgGln: 4.03 ± 0.092	6.608ArgArg: 6.608 ± 0.125	5.098ArgSer: 5.098 ± 0.09	3.052ArgThr: 3.052 ± 0.065	4.504ArgVal: 4.504 ± 0.071	1.894ArgTrp: 1.894 ± 0.06	1.666ArgTyr: 1.666 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.541SerAla: 6.541 ± 0.097	0.831SerCys: 0.831 ± 0.036	3.692SerAsp: 3.692 ± 0.104	3.446SerGlu: 3.446 ± 0.074	2.148SerPhe: 2.148 ± 0.064	6.099SerGly: 6.099 ± 0.111	1.305SerHis: 1.305 ± 0.044	2.706SerIle: 2.706 ± 0.071	1.607SerLys: 1.607 ± 0.049	7.926SerLeu: 7.926 ± 0.129	1.54SerMet: 1.54 ± 0.045	1.8SerAsn: 1.8 ± 0.063	3.194SerPro: 3.194 ± 0.066	2.508SerGln: 2.508 ± 0.053	4.473SerArg: 4.473 ± 0.082	4.729SerSer: 4.729 ± 0.133	3.569SerThr: 3.569 ± 0.091	4.44SerVal: 4.44 ± 0.09	1.217SerTrp: 1.217 ± 0.044	1.183SerTyr: 1.183 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	5.414ThrAla: 5.414 ± 0.087	0.505ThrCys: 0.505 ± 0.03	2.381ThrAsp: 2.381 ± 0.063	2.17ThrGlu: 2.17 ± 0.062	1.519ThrPhe: 1.519 ± 0.05	4.225ThrGly: 4.225 ± 0.102	0.915ThrHis: 0.915 ± 0.036	2.244ThrIle: 2.244 ± 0.078	1.119ThrLys: 1.119 ± 0.042	6.513ThrLeu: 6.513 ± 0.097	0.856ThrMet: 0.856 ± 0.036	1.213ThrAsn: 1.213 ± 0.049	3.495ThrPro: 3.495 ± 0.078	1.679ThrGln: 1.679 ± 0.048	2.87ThrArg: 2.87 ± 0.075	3.126ThrSer: 3.126 ± 0.077	2.795ThrThr: 2.795 ± 0.063	3.9ThrVal: 3.9 ± 0.082	0.686ThrTrp: 0.686 ± 0.033	1.047ThrTyr: 1.047 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	7.386ValAla: 7.386 ± 0.124	0.744ValCys: 0.744 ± 0.032	4.004ValAsp: 4.004 ± 0.096	4.184ValGlu: 4.184 ± 0.102	2.277ValPhe: 2.277 ± 0.063	5.207ValGly: 5.207 ± 0.098	1.344ValHis: 1.344 ± 0.049	3.759ValIle: 3.759 ± 0.091	1.86ValLys: 1.86 ± 0.051	9.396ValLeu: 9.396 ± 0.142	1.742ValMet: 1.742 ± 0.05	2.147ValAsn: 2.147 ± 0.054	3.524ValPro: 3.524 ± 0.088	2.93ValGln: 2.93 ± 0.064	4.171ValArg: 4.171 ± 0.086	4.301ValSer: 4.301 ± 0.086	3.519ValThr: 3.519 ± 0.079	6.486ValVal: 6.486 ± 0.123	0.901ValTrp: 0.901 ± 0.035	1.147ValTyr: 1.147 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.337TrpAla: 1.337 ± 0.047	0.253TrpCys: 0.253 ± 0.023	0.748TrpAsp: 0.748 ± 0.035	0.689TrpGlu: 0.689 ± 0.027	0.604TrpPhe: 0.604 ± 0.031	1.24TrpGly: 1.24 ± 0.044	0.387TrpHis: 0.387 ± 0.027	0.935TrpIle: 0.935 ± 0.039	0.447TrpLys: 0.447 ± 0.026	2.708TrpLeu: 2.708 ± 0.075	0.526TrpMet: 0.526 ± 0.029	0.484TrpAsn: 0.484 ± 0.029	0.991TrpPro: 0.991 ± 0.038	0.912TrpGln: 0.912 ± 0.038	1.451TrpArg: 1.451 ± 0.051	1.291TrpSer: 1.291 ± 0.044	0.862TrpThr: 0.862 ± 0.033	1.027TrpVal: 1.027 ± 0.038	0.424TrpTrp: 0.424 ± 0.03	0.291TrpTyr: 0.291 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.823TyrAla: 1.823 ± 0.051	0.249TyrCys: 0.249 ± 0.019	0.975TyrAsp: 0.975 ± 0.038	0.922TyrGlu: 0.922 ± 0.036	0.585TyrPhe: 0.585 ± 0.024	1.86TyrGly: 1.86 ± 0.057	0.331TyrHis: 0.331 ± 0.021	0.573TyrIle: 0.573 ± 0.027	0.475TyrLys: 0.475 ± 0.028	2.036TyrLeu: 2.036 ± 0.051	0.257TyrMet: 0.257 ± 0.02	0.509TyrAsn: 0.509 ± 0.028	1.001TyrPro: 1.001 ± 0.036	0.755TyrGln: 0.755 ± 0.036	1.618TyrArg: 1.618 ± 0.046	1.239TyrSer: 1.239 ± 0.047	0.759TyrThr: 0.759 ± 0.037	1.212TyrVal: 1.212 ± 0.041	0.353TyrTrp: 0.353 ± 0.024	0.406TyrTyr: 0.406 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2530 proteins (731277 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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