Halococcus hamelinensis 100A6

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halococcaceae; Halococcus; Halococcus hamelinensis

Average proteome isoelectric point is 5.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M0M748|M0M748_9EURY Na+/H+ antiporter OS=Halococcus hamelinensis 100A6 OX=1132509 GN=C447_04717 PE=4 SV=1
MM1 pKa = 6.93TTTAPDD7 pKa = 3.33RR8 pKa = 11.84FGGALCPTITPFADD22 pKa = 4.0DD23 pKa = 3.87EE24 pKa = 4.82VSTAGLEE31 pKa = 4.07TLIEE35 pKa = 4.12HH36 pKa = 6.43VIEE39 pKa = 4.69GGIEE43 pKa = 4.01GLVPCGTTGEE53 pKa = 4.24FASLSTTEE61 pKa = 3.83YY62 pKa = 8.81RR63 pKa = 11.84TVIEE67 pKa = 4.37TTVDD71 pKa = 3.35VADD74 pKa = 3.81GRR76 pKa = 11.84VPVMAGTAGTSVAGTVEE93 pKa = 4.38NIAFAAEE100 pKa = 3.96AGADD104 pKa = 3.41AALITLPYY112 pKa = 9.95FHH114 pKa = 7.05TANDD118 pKa = 3.6EE119 pKa = 4.35AGNEE123 pKa = 3.96AFITAVADD131 pKa = 4.0EE132 pKa = 4.76AALPCYY138 pKa = 10.06LYY140 pKa = 10.52NIPSCTGAPIAPEE153 pKa = 4.17TVSTVAGHH161 pKa = 6.94DD162 pKa = 3.72SVVGLKK168 pKa = 10.75DD169 pKa = 3.17SGGDD173 pKa = 3.21FTYY176 pKa = 10.22FQDD179 pKa = 3.54IVEE182 pKa = 4.28RR183 pKa = 11.84TPADD187 pKa = 3.52FQVYY191 pKa = 9.41PGSDD195 pKa = 3.2SLLAWGLLGGAAGGVCALSNVVPEE219 pKa = 4.59AFAEE223 pKa = 4.13LVAAVEE229 pKa = 4.27RR230 pKa = 11.84GDD232 pKa = 4.26IEE234 pKa = 4.46TATDD238 pKa = 3.87LQAGITALFQACVAHH253 pKa = 6.73GFAPTAKK260 pKa = 10.17AGLAVRR266 pKa = 11.84GVLPEE271 pKa = 3.87ATVRR275 pKa = 11.84PPLVEE280 pKa = 4.13LDD282 pKa = 3.09GDD284 pKa = 3.59ARR286 pKa = 11.84DD287 pKa = 3.95AVEE290 pKa = 3.9TAIDD294 pKa = 3.79DD295 pKa = 3.73VLTAVEE301 pKa = 4.61ASAA304 pKa = 4.93

Molecular weight: 30.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M0LWE5|M0LWE5_9EURY Uncharacterized protein OS=Halococcus hamelinensis 100A6 OX=1132509 GN=C447_12485 PE=4 SV=1
MM1 pKa = 7.56GARR4 pKa = 11.84SRR6 pKa = 11.84WPCVIHH12 pKa = 7.12RR13 pKa = 11.84FHH15 pKa = 7.1KK16 pKa = 10.25NARR19 pKa = 11.84KK20 pKa = 9.53RR21 pKa = 11.84LRR23 pKa = 11.84EE24 pKa = 4.09LEE26 pKa = 3.98TRR28 pKa = 11.84PLIKK32 pKa = 10.4HH33 pKa = 6.08CVCAPYY39 pKa = 10.88DD40 pKa = 3.53HH41 pKa = 6.99AHH43 pKa = 6.55NARR46 pKa = 11.84IDD48 pKa = 3.65EE49 pKa = 4.97DD50 pKa = 4.03IYY52 pKa = 11.16AQRR55 pKa = 11.84SMTEE59 pKa = 4.2TVNSAMKK66 pKa = 10.28RR67 pKa = 11.84SLGYY71 pKa = 9.22VVRR74 pKa = 11.84ACDD77 pKa = 2.9WWRR80 pKa = 11.84VPRR83 pKa = 11.84DD84 pKa = 3.36RR85 pKa = 11.84SDD87 pKa = 3.64VYY89 pKa = 11.02CLQHH93 pKa = 4.48QTLRR97 pKa = 11.84HH98 pKa = 4.63TVKK101 pKa = 9.71STALQRR107 pKa = 11.84FNRR110 pKa = 11.84AKK112 pKa = 9.23QTT114 pKa = 3.42

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3400

0

3400

957097

29

1650

281.5

30.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.905 ± 0.059

0.731 ± 0.013

7.95 ± 0.056

8.171 ± 0.061

3.503 ± 0.031

8.906 ± 0.045

2.025 ± 0.021

3.925 ± 0.034

1.668 ± 0.027

9.084 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.802 ± 0.018

2.436 ± 0.026

4.698 ± 0.032

2.227 ± 0.028

6.658 ± 0.047

5.577 ± 0.038

6.574 ± 0.036

9.299 ± 0.051

1.165 ± 0.018

2.691 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski