Aneurinibacillus sp. XH2
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3424 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A120HSJ0|A0A120HSJ0_9BACL Phage_base_V domain-containing protein OS=Aneurinibacillus sp. XH2 OX=1450761 GN=ACH33_11830 PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 4.24 EE3 pKa = 4.81 AKK5 pKa = 10.65 LGSIYY10 pKa = 10.14 QAIAQNIIEE19 pKa = 5.17 TIPEE23 pKa = 4.12 DD24 pKa = 3.36 WDD26 pKa = 3.58 KK27 pKa = 11.7 VYY29 pKa = 10.88 LYY31 pKa = 11.2 AEE33 pKa = 4.1 LTEE36 pKa = 4.63 GVRR39 pKa = 11.84 KK40 pKa = 10.08 SYY42 pKa = 10.55 FFYY45 pKa = 11.21 YY46 pKa = 9.67 PVEE49 pKa = 4.19 SDD51 pKa = 3.6 EE52 pKa = 4.5 PVYY55 pKa = 11.31 SHH57 pKa = 7.86 DD58 pKa = 3.51 IPEE61 pKa = 4.48 LFDD64 pKa = 4.03 INEE67 pKa = 4.01 NEE69 pKa = 4.12 YY70 pKa = 11.17 DD71 pKa = 3.79 QLWYY75 pKa = 10.75 QLLDD79 pKa = 3.77 LLKK82 pKa = 10.49 EE83 pKa = 3.9 LWDD86 pKa = 3.7 EE87 pKa = 4.39 FKK89 pKa = 11.48 VNSNQPWSNLTLILDD104 pKa = 3.62 NEE106 pKa = 4.87 GNFKK110 pKa = 9.88 IDD112 pKa = 3.12 YY113 pKa = 10.08 SYY115 pKa = 12.08 NDD117 pKa = 3.76 LSKK120 pKa = 11.07 ADD122 pKa = 3.61 PHH124 pKa = 5.85 EE125 pKa = 3.92 QQIIWEE131 pKa = 4.24 HH132 pKa = 6.64 KK133 pKa = 8.38 YY134 pKa = 10.85 LGLIPEE140 pKa = 5.2 DD141 pKa = 4.14 DD142 pKa = 4.34 DD143 pKa = 4.98 DD144 pKa = 6.24 RR145 pKa = 11.84 EE146 pKa = 4.08 FLEE149 pKa = 5.32 EE150 pKa = 3.96 YY151 pKa = 10.38 LKK153 pKa = 11.07 SIEE156 pKa = 4.92 DD157 pKa = 3.37 KK158 pKa = 11.31 TEE160 pKa = 3.42
Molecular weight: 19.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.973
IPC_protein 3.935
Toseland 3.745
ProMoST 4.05
Dawson 3.91
Bjellqvist 4.088
Wikipedia 3.808
Rodwell 3.77
Grimsley 3.656
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.783
EMBOSS 3.821
Sillero 4.05
Patrickios 1.901
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.956
Protein with the highest isoelectric point:
>tr|A0A120HS21|A0A120HS21_9BACL Transposase OS=Aneurinibacillus sp. XH2 OX=1450761 GN=ACH33_03225 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 8.8 PTFNPNNRR10 pKa = 11.84 KK11 pKa = 9.18 RR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.69 VHH16 pKa = 5.46 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.49 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3424
0
3424
983083
37
1913
287.1
32.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.071 ± 0.047
0.882 ± 0.014
4.579 ± 0.033
7.588 ± 0.055
4.15 ± 0.036
7.071 ± 0.041
2.116 ± 0.017
7.283 ± 0.035
6.419 ± 0.048
9.702 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.929 ± 0.023
3.709 ± 0.028
3.923 ± 0.031
3.811 ± 0.031
5.302 ± 0.039
5.326 ± 0.033
5.209 ± 0.03
7.386 ± 0.032
1.066 ± 0.015
3.476 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here