Amino acid dipepetide frequency for Aneurinibacillus sp. XH2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.472AlaAla: 7.472 ± 0.115
0.837AlaCys: 0.837 ± 0.027
3.671AlaAsp: 3.671 ± 0.065
5.959AlaGlu: 5.959 ± 0.095
3.409AlaPhe: 3.409 ± 0.055
6.166AlaGly: 6.166 ± 0.101
1.55AlaHis: 1.55 ± 0.037
5.874AlaIle: 5.874 ± 0.08
4.937AlaLys: 4.937 ± 0.077
8.375AlaLeu: 8.375 ± 0.109
2.29AlaMet: 2.29 ± 0.051
2.666AlaAsn: 2.666 ± 0.052
2.607AlaPro: 2.607 ± 0.055
2.84AlaGln: 2.84 ± 0.067
4.385AlaArg: 4.385 ± 0.078
4.355AlaSer: 4.355 ± 0.066
3.704AlaThr: 3.704 ± 0.067
6.144AlaVal: 6.144 ± 0.087
0.765AlaTrp: 0.765 ± 0.028
2.701AlaTyr: 2.701 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
0.624CysAla: 0.624 ± 0.029
0.145CysCys: 0.145 ± 0.014
0.467CysAsp: 0.467 ± 0.019
0.575CysGlu: 0.575 ± 0.024
0.368CysPhe: 0.368 ± 0.02
0.924CysGly: 0.924 ± 0.029
0.21CysHis: 0.21 ± 0.017
0.571CysIle: 0.571 ± 0.021
0.407CysLys: 0.407 ± 0.022
0.795CysLeu: 0.795 ± 0.031
0.272CysMet: 0.272 ± 0.016
0.299CysAsn: 0.299 ± 0.02
0.44CysPro: 0.44 ± 0.024
0.282CysGln: 0.282 ± 0.017
0.526CysArg: 0.526 ± 0.025
0.519CysSer: 0.519 ± 0.023
0.449CysThr: 0.449 ± 0.021
0.544CysVal: 0.544 ± 0.029
0.103CysTrp: 0.103 ± 0.01
0.299CysTyr: 0.299 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.336AspAla: 3.336 ± 0.063
0.424AspCys: 0.424 ± 0.02
1.938AspAsp: 1.938 ± 0.053
4.026AspGlu: 4.026 ± 0.071
1.993AspPhe: 1.993 ± 0.047
3.129AspGly: 3.129 ± 0.058
0.792AspHis: 0.792 ± 0.031
3.916AspIle: 3.916 ± 0.068
2.976AspLys: 2.976 ± 0.06
4.193AspLeu: 4.193 ± 0.057
1.46AspMet: 1.46 ± 0.036
1.503AspAsn: 1.503 ± 0.038
1.884AspPro: 1.884 ± 0.046
1.283AspGln: 1.283 ± 0.034
2.305AspArg: 2.305 ± 0.047
2.118AspSer: 2.118 ± 0.05
2.461AspThr: 2.461 ± 0.05
3.746AspVal: 3.746 ± 0.058
0.576AspTrp: 0.576 ± 0.025
1.735AspTyr: 1.735 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
6.559GluAla: 6.559 ± 0.105
0.465GluCys: 0.465 ± 0.023
3.184GluAsp: 3.184 ± 0.06
7.343GluGlu: 7.343 ± 0.124
2.363GluPhe: 2.363 ± 0.051
4.514GluGly: 4.514 ± 0.076
1.514GluHis: 1.514 ± 0.042
5.399GluIle: 5.399 ± 0.072
6.417GluLys: 6.417 ± 0.097
6.913GluLeu: 6.913 ± 0.107
2.509GluMet: 2.509 ± 0.054
2.998GluAsn: 2.998 ± 0.06
2.013GluPro: 2.013 ± 0.049
3.805GluGln: 3.805 ± 0.075
4.747GluArg: 4.747 ± 0.081
3.062GluSer: 3.062 ± 0.053
3.642GluThr: 3.642 ± 0.063
5.167GluVal: 5.167 ± 0.081
0.927GluTrp: 0.927 ± 0.033
2.341GluTyr: 2.341 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.348PheAla: 3.348 ± 0.067
0.42PheCys: 0.42 ± 0.02
1.89PheAsp: 1.89 ± 0.052
2.42PheGlu: 2.42 ± 0.051
2.237PhePhe: 2.237 ± 0.058
3.155PheGly: 3.155 ± 0.073
0.881PheHis: 0.881 ± 0.029
3.26PheIle: 3.26 ± 0.064
1.783PheLys: 1.783 ± 0.047
4.395PheLeu: 4.395 ± 0.083
1.099PheMet: 1.099 ± 0.035
1.333PheAsn: 1.333 ± 0.038
1.686PhePro: 1.686 ± 0.043
1.318PheGln: 1.318 ± 0.036
1.85PheArg: 1.85 ± 0.048
2.801PheSer: 2.801 ± 0.059
2.391PheThr: 2.391 ± 0.052
3.179PheVal: 3.179 ± 0.07
0.453PheTrp: 0.453 ± 0.024
1.598PheTyr: 1.598 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
5.477GlyAla: 5.477 ± 0.091
0.733GlyCys: 0.733 ± 0.031
3.002GlyAsp: 3.002 ± 0.061
4.724GlyGlu: 4.724 ± 0.071
3.145GlyPhe: 3.145 ± 0.062
5.002GlyGly: 5.002 ± 0.082
1.475GlyHis: 1.475 ± 0.038
5.833GlyIle: 5.833 ± 0.091
5.118GlyLys: 5.118 ± 0.077
6.71GlyLeu: 6.71 ± 0.106
2.39GlyMet: 2.39 ± 0.049
2.503GlyAsn: 2.503 ± 0.05
1.847GlyPro: 1.847 ± 0.043
2.41GlyGln: 2.41 ± 0.048
3.508GlyArg: 3.508 ± 0.065
3.536GlySer: 3.536 ± 0.064
4.157GlyThr: 4.157 ± 0.066
5.426GlyVal: 5.426 ± 0.075
0.925GlyTrp: 0.925 ± 0.033
2.788GlyTyr: 2.788 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
1.509HisAla: 1.509 ± 0.041
0.205HisCys: 0.205 ± 0.014
0.968HisAsp: 0.968 ± 0.034
1.322HisGlu: 1.322 ± 0.04
0.923HisPhe: 0.923 ± 0.032
1.471HisGly: 1.471 ± 0.036
0.652HisHis: 0.652 ± 0.03
1.576HisIle: 1.576 ± 0.049
1.117HisLys: 1.117 ± 0.034
2.007HisLeu: 2.007 ± 0.047
0.593HisMet: 0.593 ± 0.028
0.762HisAsn: 0.762 ± 0.028
1.35HisPro: 1.35 ± 0.042
0.725HisGln: 0.725 ± 0.026
1.113HisArg: 1.113 ± 0.035
1.155HisSer: 1.155 ± 0.033
1.149HisThr: 1.149 ± 0.037
1.524HisVal: 1.524 ± 0.036
0.226HisTrp: 0.226 ± 0.017
0.817HisTyr: 0.817 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.356IleAla: 6.356 ± 0.084
0.737IleCys: 0.737 ± 0.037
3.702IleAsp: 3.702 ± 0.058
5.369IleGlu: 5.369 ± 0.081
2.798IlePhe: 2.798 ± 0.061
5.774IleGly: 5.774 ± 0.101
1.638IleHis: 1.638 ± 0.035
5.144IleIle: 5.144 ± 0.091
3.951IleLys: 3.951 ± 0.071
6.218IleLeu: 6.218 ± 0.094
1.832IleMet: 1.832 ± 0.043
2.544IleAsn: 2.544 ± 0.057
3.417IlePro: 3.417 ± 0.063
2.692IleGln: 2.692 ± 0.057
3.768IleArg: 3.768 ± 0.067
4.384IleSer: 4.384 ± 0.067
4.095IleThr: 4.095 ± 0.061
5.557IleVal: 5.557 ± 0.078
0.664IleTrp: 0.664 ± 0.029
2.194IleTyr: 2.194 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
5.006LysAla: 5.006 ± 0.078
0.372LysCys: 0.372 ± 0.02
2.904LysAsp: 2.904 ± 0.059
6.205LysGlu: 6.205 ± 0.089
1.74LysPhe: 1.74 ± 0.042
4.021LysGly: 4.021 ± 0.064
1.269LysHis: 1.269 ± 0.03
4.16LysIle: 4.16 ± 0.063
5.469LysLys: 5.469 ± 0.075
5.406LysLeu: 5.406 ± 0.082
2.154LysMet: 2.154 ± 0.046
2.83LysAsn: 2.83 ± 0.065
2.386LysPro: 2.386 ± 0.056
3.164LysGln: 3.164 ± 0.067
3.742LysArg: 3.742 ± 0.06
2.727LysSer: 2.727 ± 0.058
3.288LysThr: 3.288 ± 0.06
4.576LysVal: 4.576 ± 0.07
0.677LysTrp: 0.677 ± 0.03
2.094LysTyr: 2.094 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
8.623LeuAla: 8.623 ± 0.096
0.884LeuCys: 0.884 ± 0.03
4.569LeuAsp: 4.569 ± 0.075
6.764LeuGlu: 6.764 ± 0.1
4.414LeuPhe: 4.414 ± 0.089
6.565LeuGly: 6.565 ± 0.103
2.213LeuHis: 2.213 ± 0.047
6.351LeuIle: 6.351 ± 0.094
5.55LeuLys: 5.55 ± 0.083
10.185LeuLeu: 10.185 ± 0.147
2.381LeuMet: 2.381 ± 0.045
3.372LeuAsn: 3.372 ± 0.059
4.317LeuPro: 4.317 ± 0.068
3.591LeuGln: 3.591 ± 0.075
5.068LeuArg: 5.068 ± 0.082
6.237LeuSer: 6.237 ± 0.092
5.269LeuThr: 5.269 ± 0.078
6.527LeuVal: 6.527 ± 0.097
0.88LeuTrp: 0.88 ± 0.033
3.258LeuTyr: 3.258 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.451MetAla: 2.451 ± 0.053
0.189MetCys: 0.189 ± 0.016
1.397MetAsp: 1.397 ± 0.033
2.272MetGlu: 2.272 ± 0.045
1.048MetPhe: 1.048 ± 0.032
1.935MetGly: 1.935 ± 0.05
0.501MetHis: 0.501 ± 0.024
2.127MetIle: 2.127 ± 0.05
2.317MetLys: 2.317 ± 0.044
2.833MetLeu: 2.833 ± 0.061
0.974MetMet: 0.974 ± 0.035
1.411MetAsn: 1.411 ± 0.037
1.085MetPro: 1.085 ± 0.041
1.092MetGln: 1.092 ± 0.033
1.503MetArg: 1.503 ± 0.038
1.623MetSer: 1.623 ± 0.043
1.451MetThr: 1.451 ± 0.038
2.032MetVal: 2.032 ± 0.044
0.228MetTrp: 0.228 ± 0.014
0.82MetTyr: 0.82 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.62AsnAla: 2.62 ± 0.054
0.337AsnCys: 0.337 ± 0.02
1.598AsnAsp: 1.598 ± 0.043
2.673AsnGlu: 2.673 ± 0.053
1.383AsnPhe: 1.383 ± 0.046
2.924AsnGly: 2.924 ± 0.059
0.823AsnHis: 0.823 ± 0.029
2.949AsnIle: 2.949 ± 0.059
2.39AsnLys: 2.39 ± 0.049
3.231AsnLeu: 3.231 ± 0.06
1.126AsnMet: 1.126 ± 0.035
1.357AsnAsn: 1.357 ± 0.037
2.042AsnPro: 2.042 ± 0.041
1.538AsnGln: 1.538 ± 0.041
2.154AsnArg: 2.154 ± 0.049
1.628AsnSer: 1.628 ± 0.041
1.83AsnThr: 1.83 ± 0.043
2.849AsnVal: 2.849 ± 0.051
0.442AsnTrp: 0.442 ± 0.024
1.195AsnTyr: 1.195 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
2.881ProAla: 2.881 ± 0.061
0.293ProCys: 0.293 ± 0.017
2.264ProAsp: 2.264 ± 0.045
3.483ProGlu: 3.483 ± 0.056
1.875ProPhe: 1.875 ± 0.045
3.055ProGly: 3.055 ± 0.063
0.928ProHis: 0.928 ± 0.034
2.499ProIle: 2.499 ± 0.048
2.041ProLys: 2.041 ± 0.052
3.859ProLeu: 3.859 ± 0.071
0.872ProMet: 0.872 ± 0.036
1.52ProAsn: 1.52 ± 0.048
1.331ProPro: 1.331 ± 0.04
1.267ProGln: 1.267 ± 0.038
1.631ProArg: 1.631 ± 0.043
2.177ProSer: 2.177 ± 0.05
1.901ProThr: 1.901 ± 0.04
3.393ProVal: 3.393 ± 0.059
0.398ProTrp: 0.398 ± 0.021
1.569ProTyr: 1.569 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
3.542GlnAla: 3.542 ± 0.066
0.221GlnCys: 0.221 ± 0.013
1.477GlnAsp: 1.477 ± 0.037
3.089GlnGlu: 3.089 ± 0.07
1.314GlnPhe: 1.314 ± 0.035
2.389GlnGly: 2.389 ± 0.049
0.814GlnHis: 0.814 ± 0.025
2.61GlnIle: 2.61 ± 0.055
2.74GlnLys: 2.74 ± 0.063
3.74GlnLeu: 3.74 ± 0.068
1.261GlnMet: 1.261 ± 0.035
1.533GlnAsn: 1.533 ± 0.042
1.335GlnPro: 1.335 ± 0.039
1.915GlnGln: 1.915 ± 0.059
2.089GlnArg: 2.089 ± 0.043
1.729GlnSer: 1.729 ± 0.047
1.941GlnThr: 1.941 ± 0.05
2.68GlnVal: 2.68 ± 0.054
0.411GlnTrp: 0.411 ± 0.02
1.277GlnTyr: 1.277 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
3.739ArgAla: 3.739 ± 0.063
0.437ArgCys: 0.437 ± 0.024
2.387ArgAsp: 2.387 ± 0.046
4.36ArgGlu: 4.36 ± 0.079
2.208ArgPhe: 2.208 ± 0.05
3.106ArgGly: 3.106 ± 0.06
1.12ArgHis: 1.12 ± 0.029
3.988ArgIle: 3.988 ± 0.061
3.783ArgLys: 3.783 ± 0.057
5.406ArgLeu: 5.406 ± 0.085
1.802ArgMet: 1.802 ± 0.043
2.089ArgAsn: 2.089 ± 0.053
1.835ArgPro: 1.835 ± 0.05
2.286ArgGln: 2.286 ± 0.052
3.139ArgArg: 3.139 ± 0.068
2.52ArgSer: 2.52 ± 0.053
2.572ArgThr: 2.572 ± 0.044
3.585ArgVal: 3.585 ± 0.062
0.6ArgTrp: 0.6 ± 0.026
2.058ArgTyr: 2.058 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
3.975SerAla: 3.975 ± 0.07
0.472SerCys: 0.472 ± 0.02
2.309SerAsp: 2.309 ± 0.046
3.387SerGlu: 3.387 ± 0.053
2.703SerPhe: 2.703 ± 0.057
4.158SerGly: 4.158 ± 0.067
1.203SerHis: 1.203 ± 0.038
3.895SerIle: 3.895 ± 0.064
2.963SerLys: 2.963 ± 0.055
5.693SerLeu: 5.693 ± 0.077
1.53SerMet: 1.53 ± 0.039
1.817SerAsn: 1.817 ± 0.046
2.181SerPro: 2.181 ± 0.051
1.878SerGln: 1.878 ± 0.041
2.741SerArg: 2.741 ± 0.059
3.209SerSer: 3.209 ± 0.067
2.615SerThr: 2.615 ± 0.051
3.977SerVal: 3.977 ± 0.061
0.573SerTrp: 0.573 ± 0.023
1.981SerTyr: 1.981 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.059ThrAla: 4.059 ± 0.066
0.414ThrCys: 0.414 ± 0.023
2.338ThrAsp: 2.338 ± 0.047
3.442ThrGlu: 3.442 ± 0.062
2.422ThrPhe: 2.422 ± 0.047
4.376ThrGly: 4.376 ± 0.073
0.984ThrHis: 0.984 ± 0.03
4.004ThrIle: 4.004 ± 0.064
2.933ThrLys: 2.933 ± 0.061
5.167ThrLeu: 5.167 ± 0.079
1.416ThrMet: 1.416 ± 0.039
1.934ThrAsn: 1.934 ± 0.047
2.425ThrPro: 2.425 ± 0.058
1.586ThrGln: 1.586 ± 0.044
2.404ThrArg: 2.404 ± 0.048
2.795ThrSer: 2.795 ± 0.056
2.635ThrThr: 2.635 ± 0.063
4.314ThrVal: 4.314 ± 0.068
0.52ThrTrp: 0.52 ± 0.026
1.928ThrTyr: 1.928 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
5.974ValAla: 5.974 ± 0.094
0.746ValCys: 0.746 ± 0.028
3.519ValAsp: 3.519 ± 0.057
5.2ValGlu: 5.2 ± 0.083
3.042ValPhe: 3.042 ± 0.065
4.808ValGly: 4.808 ± 0.085
1.52ValHis: 1.52 ± 0.041
5.49ValIle: 5.49 ± 0.088
4.557ValLys: 4.557 ± 0.072
7.263ValLeu: 7.263 ± 0.105
2.07ValMet: 2.07 ± 0.039
2.776ValAsn: 2.776 ± 0.052
3.236ValPro: 3.236 ± 0.066
2.651ValGln: 2.651 ± 0.053
3.819ValArg: 3.819 ± 0.057
4.278ValSer: 4.278 ± 0.072
4.185ValThr: 4.185 ± 0.068
5.545ValVal: 5.545 ± 0.087
0.782ValTrp: 0.782 ± 0.03
2.404ValTyr: 2.404 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
0.717TrpAla: 0.717 ± 0.028
0.1TrpCys: 0.1 ± 0.009
0.522TrpAsp: 0.522 ± 0.024
0.741TrpGlu: 0.741 ± 0.029
0.506TrpPhe: 0.506 ± 0.023
0.701TrpGly: 0.701 ± 0.026
0.208TrpHis: 0.208 ± 0.016
0.767TrpIle: 0.767 ± 0.03
0.771TrpLys: 0.771 ± 0.026
1.235TrpLeu: 1.235 ± 0.045
0.42TrpMet: 0.42 ± 0.021
0.504TrpAsn: 0.504 ± 0.023
0.315TrpPro: 0.315 ± 0.019
0.426TrpGln: 0.426 ± 0.021
0.528TrpArg: 0.528 ± 0.023
0.51TrpSer: 0.51 ± 0.025
0.522TrpThr: 0.522 ± 0.024
0.686TrpVal: 0.686 ± 0.028
0.146TrpTrp: 0.146 ± 0.014
0.335TrpTyr: 0.335 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.439TyrAla: 2.439 ± 0.045
0.385TyrCys: 0.385 ± 0.019
1.69TyrAsp: 1.69 ± 0.043
2.527TyrGlu: 2.527 ± 0.051
1.606TyrPhe: 1.606 ± 0.043
2.535TyrGly: 2.535 ± 0.051
0.869TyrHis: 0.869 ± 0.035
2.419TyrIle: 2.419 ± 0.053
1.975TyrLys: 1.975 ± 0.049
3.393TyrLeu: 3.393 ± 0.069
0.838TyrMet: 0.838 ± 0.031
1.341TyrAsn: 1.341 ± 0.042
1.503TyrPro: 1.503 ± 0.042
1.36TyrGln: 1.36 ± 0.039
2.0TyrArg: 2.0 ± 0.05
1.899TyrSer: 1.899 ± 0.045
1.838TyrThr: 1.838 ± 0.048
2.411TyrVal: 2.411 ± 0.046
0.362TyrTrp: 0.362 ± 0.021
1.374TyrTyr: 1.374 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3424 proteins (983083 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski