Thiomonas delicata (Thiomonas cuprina)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Thiomonas

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3683 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238D3W1|A0A238D3W1_THIDL Hydroxypyruvate isomerase OS=Thiomonas delicata OX=364030 GN=hyi PE=3 SV=1
MM1 pKa = 6.64NTEE4 pKa = 4.81FAASGPPHH12 pKa = 7.35APPDD16 pKa = 3.91SPVDD20 pKa = 3.56DD21 pKa = 3.7TRR23 pKa = 11.84QWVCKK28 pKa = 10.38VCGLVYY34 pKa = 10.65DD35 pKa = 5.19PVAGMPDD42 pKa = 3.47DD43 pKa = 5.63GIPAGTRR50 pKa = 11.84FEE52 pKa = 5.69DD53 pKa = 5.31IPDD56 pKa = 2.96GWYY59 pKa = 10.87CPDD62 pKa = 4.11CGVTKK67 pKa = 10.73ADD69 pKa = 3.83FEE71 pKa = 4.85LLVFF75 pKa = 4.98

Molecular weight: 8.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238D194|A0A238D194_THIDL Uncharacterized protein OS=Thiomonas delicata OX=364030 GN=THIARS_50222 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.98TRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.99RR14 pKa = 11.84THH16 pKa = 5.86GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TVGGRR28 pKa = 11.84AVIKK32 pKa = 10.38ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3683

0

3683

1131413

20

2912

307.2

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.346 ± 0.071

0.966 ± 0.013

5.156 ± 0.035

4.827 ± 0.044

3.345 ± 0.026

8.221 ± 0.044

2.516 ± 0.021

4.249 ± 0.03

2.808 ± 0.032

11.192 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.019

2.423 ± 0.025

5.619 ± 0.029

4.431 ± 0.034

7.402 ± 0.044

5.153 ± 0.031

4.889 ± 0.025

7.25 ± 0.037

1.543 ± 0.02

2.173 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski