Pseudomonas phage PFP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pifdecavirus; Pseudomonas virus PFP1

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QIN7|A0A2Z4QIN7_9CAUD RNA polymerase inhibitor OS=Pseudomonas phage PFP1 OX=2201462 GN=PFP1_13 PE=4 SV=1
MM1 pKa = 7.06TVWTHH6 pKa = 3.71WCEE9 pKa = 3.25WDD11 pKa = 3.31MGFEE15 pKa = 4.08GAVWEE20 pKa = 4.34TLEE23 pKa = 4.44AAQRR27 pKa = 11.84DD28 pKa = 3.88LRR30 pKa = 11.84IAFEE34 pKa = 4.23NMGEE38 pKa = 4.02DD39 pKa = 3.9FEE41 pKa = 6.16DD42 pKa = 3.87SVEE45 pKa = 4.08AGLLGFDD52 pKa = 3.55STEE55 pKa = 3.95VLVV58 pKa = 3.98

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4QJ77|A0A2Z4QJ77_9CAUD Endonuclease I OS=Pseudomonas phage PFP1 OX=2201462 GN=PFP1_16 PE=4 SV=1
MM1 pKa = 7.75NDD3 pKa = 3.37MTRR6 pKa = 11.84KK7 pKa = 8.81AAKK10 pKa = 9.36PGLYY14 pKa = 8.5DD15 pKa = 3.41TQLRR19 pKa = 11.84SAKK22 pKa = 10.25AFRR25 pKa = 11.84NVVNLKK31 pKa = 10.36KK32 pKa = 10.73RR33 pKa = 11.84GDD35 pKa = 3.75RR36 pKa = 11.84DD37 pKa = 3.65DD38 pKa = 5.94LMVSLADD45 pKa = 3.72AQHH48 pKa = 5.51GRR50 pKa = 11.84MRR52 pKa = 11.84YY53 pKa = 3.97TTKK56 pKa = 11.14GDD58 pKa = 3.28FTNCFRR64 pKa = 11.84RR65 pKa = 11.84GII67 pKa = 3.84

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12054

38

1331

267.9

29.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.046 ± 0.445

0.805 ± 0.158

6.346 ± 0.231

6.911 ± 0.365

3.692 ± 0.192

8.147 ± 0.384

2.281 ± 0.172

4.48 ± 0.165

6.114 ± 0.328

8.246 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.02 ± 0.253

3.675 ± 0.234

3.866 ± 0.17

4.123 ± 0.3

5.84 ± 0.166

5.608 ± 0.288

5.558 ± 0.179

6.869 ± 0.364

1.427 ± 0.176

2.945 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski