Amino acid dipepetide frequency for Pseudomonas phage PFP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.118AlaAla: 11.118 ± 1.135
0.747AlaCys: 0.747 ± 0.241
7.135AlaAsp: 7.135 ± 0.805
6.057AlaGlu: 6.057 ± 0.681
3.402AlaPhe: 3.402 ± 0.57
7.882AlaGly: 7.882 ± 0.579
2.24AlaHis: 2.24 ± 0.434
3.651AlaIle: 3.651 ± 0.411
6.554AlaLys: 6.554 ± 0.511
8.297AlaLeu: 8.297 ± 0.764
3.816AlaMet: 3.816 ± 0.574
3.816AlaAsn: 3.816 ± 0.93
2.987AlaPro: 2.987 ± 0.47
5.144AlaGln: 5.144 ± 0.719
6.14AlaArg: 6.14 ± 0.661
5.808AlaSer: 5.808 ± 0.715
4.978AlaThr: 4.978 ± 0.872
7.218AlaVal: 7.218 ± 0.588
1.245AlaTrp: 1.245 ± 0.403
2.24AlaTyr: 2.24 ± 0.508
0.0AlaXaa: 0.0 ± 0.0
Cys
1.162CysAla: 1.162 ± 0.312
0.0CysCys: 0.0 ± 0.0
0.913CysAsp: 0.913 ± 0.369
0.415CysGlu: 0.415 ± 0.194
0.498CysPhe: 0.498 ± 0.2
0.415CysGly: 0.415 ± 0.174
0.166CysHis: 0.166 ± 0.101
0.332CysIle: 0.332 ± 0.169
0.498CysLys: 0.498 ± 0.199
0.664CysLeu: 0.664 ± 0.247
0.166CysMet: 0.166 ± 0.115
0.249CysAsn: 0.249 ± 0.137
0.498CysPro: 0.498 ± 0.204
0.332CysGln: 0.332 ± 0.15
0.415CysArg: 0.415 ± 0.175
0.166CysSer: 0.166 ± 0.125
0.166CysThr: 0.166 ± 0.094
0.581CysVal: 0.581 ± 0.234
0.083CysTrp: 0.083 ± 0.094
0.332CysTyr: 0.332 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
7.882AspAla: 7.882 ± 0.828
0.249AspCys: 0.249 ± 0.175
3.982AspAsp: 3.982 ± 0.668
3.568AspGlu: 3.568 ± 0.613
3.402AspPhe: 3.402 ± 0.456
6.72AspGly: 6.72 ± 0.833
1.41AspHis: 1.41 ± 0.319
3.568AspIle: 3.568 ± 0.381
2.572AspLys: 2.572 ± 0.53
4.895AspLeu: 4.895 ± 0.718
1.908AspMet: 1.908 ± 0.39
1.576AspAsn: 1.576 ± 0.227
2.821AspPro: 2.821 ± 0.58
2.323AspGln: 2.323 ± 0.457
4.065AspArg: 4.065 ± 0.392
2.738AspSer: 2.738 ± 0.562
2.821AspThr: 2.821 ± 0.485
4.231AspVal: 4.231 ± 0.53
0.996AspTrp: 0.996 ± 0.309
1.742AspTyr: 1.742 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
9.375GluAla: 9.375 ± 0.941
0.913GluCys: 0.913 ± 0.292
4.065GluAsp: 4.065 ± 0.505
5.227GluGlu: 5.227 ± 0.833
2.489GluPhe: 2.489 ± 0.446
5.725GluGly: 5.725 ± 0.731
1.327GluHis: 1.327 ± 0.311
3.153GluIle: 3.153 ± 0.488
2.323GluLys: 2.323 ± 0.424
5.476GluLeu: 5.476 ± 0.653
1.41GluMet: 1.41 ± 0.415
2.821GluAsn: 2.821 ± 0.418
1.908GluPro: 1.908 ± 0.413
2.655GluGln: 2.655 ± 0.416
3.899GluArg: 3.899 ± 0.437
3.568GluSer: 3.568 ± 0.527
4.314GluThr: 4.314 ± 0.442
5.061GluVal: 5.061 ± 0.756
1.41GluTrp: 1.41 ± 0.303
1.991GluTyr: 1.991 ± 0.34
0.0GluXaa: 0.0 ± 0.0
Phe
2.489PheAla: 2.489 ± 0.465
0.415PheCys: 0.415 ± 0.216
2.821PheAsp: 2.821 ± 0.514
2.157PheGlu: 2.157 ± 0.467
1.245PhePhe: 1.245 ± 0.288
2.655PheGly: 2.655 ± 0.419
1.162PheHis: 1.162 ± 0.244
1.327PheIle: 1.327 ± 0.34
1.991PheLys: 1.991 ± 0.449
4.148PheLeu: 4.148 ± 0.575
1.327PheMet: 1.327 ± 0.232
1.742PheAsn: 1.742 ± 0.331
1.41PhePro: 1.41 ± 0.378
1.493PheGln: 1.493 ± 0.375
2.489PheArg: 2.489 ± 0.469
2.074PheSer: 2.074 ± 0.401
2.074PheThr: 2.074 ± 0.312
2.572PheVal: 2.572 ± 0.361
0.498PheTrp: 0.498 ± 0.182
0.83PheTyr: 0.83 ± 0.283
0.0PheXaa: 0.0 ± 0.0
Gly
6.886GlyAla: 6.886 ± 1.091
0.83GlyCys: 0.83 ± 0.259
5.31GlyAsp: 5.31 ± 0.675
5.393GlyGlu: 5.393 ± 0.639
3.651GlyPhe: 3.651 ± 0.599
6.305GlyGly: 6.305 ± 0.725
2.157GlyHis: 2.157 ± 0.309
4.729GlyIle: 4.729 ± 0.753
5.227GlyLys: 5.227 ± 0.961
6.388GlyLeu: 6.388 ± 0.81
1.659GlyMet: 1.659 ± 0.42
3.153GlyAsn: 3.153 ± 0.536
2.323GlyPro: 2.323 ± 0.41
2.323GlyGln: 2.323 ± 0.333
5.476GlyArg: 5.476 ± 0.743
5.808GlySer: 5.808 ± 0.605
4.646GlyThr: 4.646 ± 0.469
4.895GlyVal: 4.895 ± 0.644
1.245GlyTrp: 1.245 ± 0.326
3.07GlyTyr: 3.07 ± 0.507
0.0GlyXaa: 0.0 ± 0.0
His
1.742HisAla: 1.742 ± 0.411
0.415HisCys: 0.415 ± 0.157
1.162HisAsp: 1.162 ± 0.31
2.406HisGlu: 2.406 ± 0.363
0.996HisPhe: 0.996 ± 0.23
1.908HisGly: 1.908 ± 0.458
0.415HisHis: 0.415 ± 0.177
0.913HisIle: 0.913 ± 0.384
1.576HisLys: 1.576 ± 0.32
2.074HisLeu: 2.074 ± 0.41
0.747HisMet: 0.747 ± 0.261
1.162HisAsn: 1.162 ± 0.308
0.996HisPro: 0.996 ± 0.34
0.498HisGln: 0.498 ± 0.179
1.162HisArg: 1.162 ± 0.322
1.079HisSer: 1.079 ± 0.313
0.664HisThr: 0.664 ± 0.252
1.493HisVal: 1.493 ± 0.361
0.581HisTrp: 0.581 ± 0.199
0.83HisTyr: 0.83 ± 0.22
0.0HisXaa: 0.0 ± 0.0
Ile
4.148IleAla: 4.148 ± 0.561
0.498IleCys: 0.498 ± 0.184
2.24IleAsp: 2.24 ± 0.39
2.821IleGlu: 2.821 ± 0.49
0.664IlePhe: 0.664 ± 0.209
3.816IleGly: 3.816 ± 0.506
1.327IleHis: 1.327 ± 0.391
1.991IleIle: 1.991 ± 0.333
3.651IleLys: 3.651 ± 0.462
3.899IleLeu: 3.899 ± 0.556
1.41IleMet: 1.41 ± 0.346
1.162IleAsn: 1.162 ± 0.339
2.655IlePro: 2.655 ± 0.489
2.738IleGln: 2.738 ± 0.431
2.24IleArg: 2.24 ± 0.328
1.825IleSer: 1.825 ± 0.326
2.821IleThr: 2.821 ± 0.57
2.904IleVal: 2.904 ± 0.542
0.664IleTrp: 0.664 ± 0.206
1.327IleTyr: 1.327 ± 0.368
0.0IleXaa: 0.0 ± 0.0
Lys
7.965LysAla: 7.965 ± 0.976
0.415LysCys: 0.415 ± 0.167
4.397LysAsp: 4.397 ± 0.998
3.651LysGlu: 3.651 ± 0.633
1.991LysPhe: 1.991 ± 0.275
4.812LysGly: 4.812 ± 0.682
1.576LysHis: 1.576 ± 0.443
2.323LysIle: 2.323 ± 0.358
3.402LysLys: 3.402 ± 0.967
5.061LysLeu: 5.061 ± 0.614
1.908LysMet: 1.908 ± 0.301
1.742LysAsn: 1.742 ± 0.404
2.987LysPro: 2.987 ± 0.663
2.406LysGln: 2.406 ± 0.482
3.734LysArg: 3.734 ± 0.751
3.402LysSer: 3.402 ± 0.491
3.402LysThr: 3.402 ± 0.543
4.065LysVal: 4.065 ± 0.662
0.415LysTrp: 0.415 ± 0.157
1.493LysTyr: 1.493 ± 0.384
0.0LysXaa: 0.0 ± 0.0
Leu
7.716LeuAla: 7.716 ± 0.679
0.581LeuCys: 0.581 ± 0.242
4.48LeuAsp: 4.48 ± 0.774
6.637LeuGlu: 6.637 ± 0.817
2.738LeuPhe: 2.738 ± 0.582
5.061LeuGly: 5.061 ± 0.737
1.41LeuHis: 1.41 ± 0.324
4.314LeuIle: 4.314 ± 0.601
6.72LeuLys: 6.72 ± 0.69
5.559LeuLeu: 5.559 ± 0.805
2.489LeuMet: 2.489 ± 0.541
4.314LeuAsn: 4.314 ± 0.486
2.904LeuPro: 2.904 ± 0.435
3.568LeuGln: 3.568 ± 0.626
4.729LeuArg: 4.729 ± 0.512
4.314LeuSer: 4.314 ± 0.616
5.227LeuThr: 5.227 ± 0.554
6.305LeuVal: 6.305 ± 0.552
1.079LeuTrp: 1.079 ± 0.373
2.323LeuTyr: 2.323 ± 0.339
0.0LeuXaa: 0.0 ± 0.0
Met
3.07MetAla: 3.07 ± 0.541
0.332MetCys: 0.332 ± 0.194
1.576MetAsp: 1.576 ± 0.277
2.489MetGlu: 2.489 ± 0.415
0.498MetPhe: 0.498 ± 0.196
2.904MetGly: 2.904 ± 0.52
0.747MetHis: 0.747 ± 0.296
1.327MetIle: 1.327 ± 0.235
1.825MetLys: 1.825 ± 0.422
2.406MetLeu: 2.406 ± 0.485
0.581MetMet: 0.581 ± 0.205
0.747MetAsn: 0.747 ± 0.229
1.41MetPro: 1.41 ± 0.308
0.913MetGln: 0.913 ± 0.294
1.41MetArg: 1.41 ± 0.322
2.24MetSer: 2.24 ± 0.371
2.572MetThr: 2.572 ± 0.485
2.074MetVal: 2.074 ± 0.292
0.498MetTrp: 0.498 ± 0.207
0.581MetTyr: 0.581 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
3.651AsnAla: 3.651 ± 0.528
0.332AsnCys: 0.332 ± 0.159
1.659AsnAsp: 1.659 ± 0.288
2.406AsnGlu: 2.406 ± 0.382
1.245AsnPhe: 1.245 ± 0.338
3.734AsnGly: 3.734 ± 0.497
0.664AsnHis: 0.664 ± 0.228
1.659AsnIle: 1.659 ± 0.446
1.493AsnLys: 1.493 ± 0.32
3.816AsnLeu: 3.816 ± 0.42
1.576AsnMet: 1.576 ± 0.349
0.664AsnAsn: 0.664 ± 0.188
3.153AsnPro: 3.153 ± 0.703
1.245AsnGln: 1.245 ± 0.26
2.406AsnArg: 2.406 ± 0.511
1.245AsnSer: 1.245 ± 0.368
1.576AsnThr: 1.576 ± 0.403
2.489AsnVal: 2.489 ± 0.495
0.415AsnTrp: 0.415 ± 0.209
1.327AsnTyr: 1.327 ± 0.346
0.0AsnXaa: 0.0 ± 0.0
Pro
2.738ProAla: 2.738 ± 0.524
0.083ProCys: 0.083 ± 0.072
2.987ProAsp: 2.987 ± 0.581
4.231ProGlu: 4.231 ± 0.66
1.493ProPhe: 1.493 ± 0.283
2.406ProGly: 2.406 ± 0.437
1.327ProHis: 1.327 ± 0.319
1.162ProIle: 1.162 ± 0.281
2.738ProLys: 2.738 ± 0.455
2.406ProLeu: 2.406 ± 0.417
1.162ProMet: 1.162 ± 0.303
2.24ProAsn: 2.24 ± 0.334
1.079ProPro: 1.079 ± 0.279
1.742ProGln: 1.742 ± 0.398
2.157ProArg: 2.157 ± 0.354
2.24ProSer: 2.24 ± 0.489
1.991ProThr: 1.991 ± 0.389
2.738ProVal: 2.738 ± 0.545
0.415ProTrp: 0.415 ± 0.218
1.327ProTyr: 1.327 ± 0.35
0.0ProXaa: 0.0 ± 0.0
Gln
4.646GlnAla: 4.646 ± 0.835
0.0GlnCys: 0.0 ± 0.0
3.236GlnAsp: 3.236 ± 0.587
2.323GlnGlu: 2.323 ± 0.403
1.825GlnPhe: 1.825 ± 0.382
3.485GlnGly: 3.485 ± 0.586
0.996GlnHis: 0.996 ± 0.32
1.576GlnIle: 1.576 ± 0.323
2.406GlnLys: 2.406 ± 0.552
3.734GlnLeu: 3.734 ± 0.563
1.41GlnMet: 1.41 ± 0.371
1.162GlnAsn: 1.162 ± 0.375
0.83GlnPro: 0.83 ± 0.289
2.406GlnGln: 2.406 ± 0.403
2.489GlnArg: 2.489 ± 0.662
2.406GlnSer: 2.406 ± 0.385
1.576GlnThr: 1.576 ± 0.308
2.904GlnVal: 2.904 ± 0.403
0.664GlnTrp: 0.664 ± 0.279
1.162GlnTyr: 1.162 ± 0.324
0.0GlnXaa: 0.0 ± 0.0
Arg
4.646ArgAla: 4.646 ± 0.623
0.498ArgCys: 0.498 ± 0.202
3.899ArgAsp: 3.899 ± 0.643
4.231ArgGlu: 4.231 ± 0.595
2.572ArgPhe: 2.572 ± 0.536
4.563ArgGly: 4.563 ± 0.557
1.327ArgHis: 1.327 ± 0.303
2.738ArgIle: 2.738 ± 0.332
3.568ArgLys: 3.568 ± 0.591
4.646ArgLeu: 4.646 ± 0.468
1.576ArgMet: 1.576 ± 0.349
2.572ArgAsn: 2.572 ± 0.395
1.825ArgPro: 1.825 ± 0.366
2.572ArgGln: 2.572 ± 0.51
3.568ArgArg: 3.568 ± 0.479
4.231ArgSer: 4.231 ± 0.617
2.821ArgThr: 2.821 ± 0.425
3.402ArgVal: 3.402 ± 0.538
0.747ArgTrp: 0.747 ± 0.31
2.323ArgTyr: 2.323 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
5.974SerAla: 5.974 ± 0.976
0.332SerCys: 0.332 ± 0.153
4.231SerAsp: 4.231 ± 0.735
2.738SerGlu: 2.738 ± 0.458
2.655SerPhe: 2.655 ± 0.334
4.978SerGly: 4.978 ± 0.62
1.327SerHis: 1.327 ± 0.278
2.904SerIle: 2.904 ± 0.371
3.651SerLys: 3.651 ± 0.382
4.646SerLeu: 4.646 ± 0.558
1.493SerMet: 1.493 ± 0.489
1.493SerAsn: 1.493 ± 0.42
1.908SerPro: 1.908 ± 0.42
1.41SerGln: 1.41 ± 0.296
2.738SerArg: 2.738 ± 0.374
3.153SerSer: 3.153 ± 0.596
2.572SerThr: 2.572 ± 0.459
4.563SerVal: 4.563 ± 0.631
0.996SerTrp: 0.996 ± 0.225
2.323SerTyr: 2.323 ± 0.561
0.0SerXaa: 0.0 ± 0.0
Thr
4.978ThrAla: 4.978 ± 0.702
0.249ThrCys: 0.249 ± 0.135
2.821ThrAsp: 2.821 ± 0.568
4.231ThrGlu: 4.231 ± 0.537
1.991ThrPhe: 1.991 ± 0.43
5.476ThrGly: 5.476 ± 0.661
1.079ThrHis: 1.079 ± 0.275
2.821ThrIle: 2.821 ± 0.459
4.563ThrLys: 4.563 ± 0.533
5.31ThrLeu: 5.31 ± 0.662
1.908ThrMet: 1.908 ± 0.398
1.908ThrAsn: 1.908 ± 0.33
2.738ThrPro: 2.738 ± 0.468
2.323ThrGln: 2.323 ± 0.569
2.157ThrArg: 2.157 ± 0.33
2.738ThrSer: 2.738 ± 0.415
2.987ThrThr: 2.987 ± 0.59
3.153ThrVal: 3.153 ± 0.476
0.415ThrTrp: 0.415 ± 0.194
1.742ThrTyr: 1.742 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
5.974ValAla: 5.974 ± 0.679
0.581ValCys: 0.581 ± 0.239
3.568ValAsp: 3.568 ± 0.511
5.974ValGlu: 5.974 ± 0.626
2.24ValPhe: 2.24 ± 0.364
4.563ValGly: 4.563 ± 0.497
1.162ValHis: 1.162 ± 0.285
2.655ValIle: 2.655 ± 0.516
3.402ValLys: 3.402 ± 0.552
5.725ValLeu: 5.725 ± 0.527
1.825ValMet: 1.825 ± 0.37
2.821ValAsn: 2.821 ± 0.481
2.904ValPro: 2.904 ± 0.298
2.904ValGln: 2.904 ± 0.426
4.48ValArg: 4.48 ± 0.591
3.816ValSer: 3.816 ± 0.414
5.725ValThr: 5.725 ± 0.615
5.227ValVal: 5.227 ± 0.742
1.162ValTrp: 1.162 ± 0.327
1.991ValTyr: 1.991 ± 0.359
0.0ValXaa: 0.0 ± 0.0
Trp
1.245TrpAla: 1.245 ± 0.303
0.166TrpCys: 0.166 ± 0.117
0.498TrpAsp: 0.498 ± 0.215
0.498TrpGlu: 0.498 ± 0.15
0.581TrpPhe: 0.581 ± 0.252
1.245TrpGly: 1.245 ± 0.221
0.664TrpHis: 0.664 ± 0.314
0.249TrpIle: 0.249 ± 0.113
1.079TrpLys: 1.079 ± 0.314
1.41TrpLeu: 1.41 ± 0.413
0.415TrpMet: 0.415 ± 0.168
0.498TrpAsn: 0.498 ± 0.214
0.415TrpPro: 0.415 ± 0.151
0.83TrpGln: 0.83 ± 0.249
0.83TrpArg: 0.83 ± 0.236
1.245TrpSer: 1.245 ± 0.41
0.996TrpThr: 0.996 ± 0.276
1.162TrpVal: 1.162 ± 0.285
0.083TrpTrp: 0.083 ± 0.082
0.166TrpTyr: 0.166 ± 0.113
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.07TyrAla: 3.07 ± 0.463
0.415TyrCys: 0.415 ± 0.185
2.489TyrAsp: 2.489 ± 0.346
1.659TyrGlu: 1.659 ± 0.318
0.747TyrPhe: 0.747 ± 0.178
2.821TyrGly: 2.821 ± 0.602
0.332TyrHis: 0.332 ± 0.152
1.41TyrIle: 1.41 ± 0.291
1.908TyrLys: 1.908 ± 0.424
1.908TyrLeu: 1.908 ± 0.408
1.327TyrMet: 1.327 ± 0.36
0.913TyrAsn: 0.913 ± 0.285
0.913TyrPro: 0.913 ± 0.236
1.41TyrGln: 1.41 ± 0.378
1.825TyrArg: 1.825 ± 0.325
1.991TyrSer: 1.991 ± 0.43
1.659TyrThr: 1.659 ± 0.375
1.659TyrVal: 1.659 ± 0.393
0.664TyrTrp: 0.664 ± 0.24
0.332TyrTyr: 0.332 ± 0.153
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (12054 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski