Streptomyces sp. SLBN-118

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A542QLB9|A0A542QLB9_9ACTN Uncharacterized protein OS=Streptomyces sp. SLBN-118 OX=2768454 GN=FBY35_1189 PE=4 SV=1
MM1 pKa = 7.7LSTRR5 pKa = 11.84FVTGSPNWIDD15 pKa = 5.02LGTPDD20 pKa = 5.41LDD22 pKa = 3.98AATAFYY28 pKa = 10.9GGLFGWTFQSAGPDD42 pKa = 2.99AGGYY46 pKa = 10.66GMYY49 pKa = 10.15QVDD52 pKa = 3.98GKK54 pKa = 8.82TVAGAMTVTPEE65 pKa = 3.74QGGPGWTVYY74 pKa = 9.86FQSPDD79 pKa = 3.14ADD81 pKa = 3.55GTAKK85 pKa = 10.44AVQAGGGAVLFEE97 pKa = 4.44PMDD100 pKa = 3.65VFEE103 pKa = 5.41LGRR106 pKa = 11.84MAVFTDD112 pKa = 3.37PGGAAFATWQPGQNKK127 pKa = 9.75GLDD130 pKa = 3.49AVNDD134 pKa = 4.28PNTLCWAEE142 pKa = 5.25LYY144 pKa = 10.4TEE146 pKa = 4.94DD147 pKa = 4.67PGAGLIFYY155 pKa = 10.17QGVFGWEE162 pKa = 3.88TSMMPLPDD170 pKa = 3.43GTGSYY175 pKa = 10.27TMINPAGAGAEE186 pKa = 4.04AMFGGIVPLAADD198 pKa = 3.9PLEE201 pKa = 4.51TEE203 pKa = 4.17GPYY206 pKa = 9.3WLPYY210 pKa = 10.42FEE212 pKa = 5.53VTDD215 pKa = 3.93CDD217 pKa = 3.65AAVATAQQLGGSVRR231 pKa = 11.84MAPVDD236 pKa = 3.5MEE238 pKa = 4.49GVGRR242 pKa = 11.84FAKK245 pKa = 10.62LADD248 pKa = 3.54PAGARR253 pKa = 11.84FALMQGVQQDD263 pKa = 3.74DD264 pKa = 3.37

Molecular weight: 27.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A542QSP5|A0A542QSP5_9ACTN Alanine racemase-like protein OS=Streptomyces sp. SLBN-118 OX=2768454 GN=FBY35_3568 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6747

0

6747

2213585

29

5832

328.1

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.305 ± 0.045

0.839 ± 0.009

5.87 ± 0.022

5.785 ± 0.029

2.724 ± 0.017

9.313 ± 0.029

2.281 ± 0.014

3.427 ± 0.021

2.42 ± 0.028

10.186 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.829 ± 0.011

1.875 ± 0.019

6.014 ± 0.028

2.978 ± 0.018

7.84 ± 0.037

5.324 ± 0.024

6.062 ± 0.03

8.293 ± 0.03

1.516 ± 0.013

2.12 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski