Vibrio azureus NBRC 104587

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio harveyi group; Vibrio azureus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3A2C3|U3A2C3_9VIBR DNA topoisomerase 3 OS=Vibrio azureus NBRC 104587 OX=1219077 GN=topB PE=3 SV=1
MM1 pKa = 7.38IAKK4 pKa = 8.17TKK6 pKa = 10.23LASLCLLSLASANIYY21 pKa = 10.16ASSVIDD27 pKa = 4.07LDD29 pKa = 4.22FSNHH33 pKa = 5.86LEE35 pKa = 4.05ATNSSVSWAGPVFTGPDD52 pKa = 3.18MHH54 pKa = 6.47FVGVGTHH61 pKa = 6.83DD62 pKa = 4.17GKK64 pKa = 9.61TIDD67 pKa = 3.85AKK69 pKa = 9.87VTSSVFGDD77 pKa = 3.84ATFLWHH83 pKa = 6.99APNYY87 pKa = 8.64NQLSGSQPLGDD98 pKa = 4.49IGFLYY103 pKa = 8.56QTNSPGNAGLTYY115 pKa = 10.63VFEE118 pKa = 4.9FFDD121 pKa = 4.07GTDD124 pKa = 3.54GLSGTFSVPYY134 pKa = 9.8IIPEE138 pKa = 4.05FEE140 pKa = 4.28FIGYY144 pKa = 9.75DD145 pKa = 3.11IDD147 pKa = 4.14GEE149 pKa = 4.46PVQSEE154 pKa = 3.95QVRR157 pKa = 11.84VFKK160 pKa = 11.31SEE162 pKa = 3.71GFYY165 pKa = 10.55SYY167 pKa = 11.3QLGSGAASLTAQEE180 pKa = 4.76SPDD183 pKa = 3.64GQSVLFTGPGTNFSEE198 pKa = 4.61TDD200 pKa = 2.76TSGAVKK206 pKa = 8.87FTYY209 pKa = 10.46KK210 pKa = 9.25NTSIVTLQFEE220 pKa = 4.87TVTTSASGFPNPIFSAFDD238 pKa = 3.93GNWDD242 pKa = 3.59LAGFDD247 pKa = 4.25TPTTSNSDD255 pKa = 3.14FDD257 pKa = 4.93YY258 pKa = 11.53GDD260 pKa = 4.2APDD263 pKa = 3.63TYY265 pKa = 9.74KK266 pKa = 10.63TLLASDD272 pKa = 5.41GPQHH276 pKa = 6.61ALSSSLYY283 pKa = 10.17LGSSVDD289 pKa = 4.23ADD291 pKa = 4.02PDD293 pKa = 3.87GQPNPTSTGDD303 pKa = 3.81DD304 pKa = 3.4FDD306 pKa = 5.72VDD308 pKa = 4.3GNDD311 pKa = 4.66DD312 pKa = 4.78DD313 pKa = 6.73GITPLTNFEE322 pKa = 4.5IGLDD326 pKa = 3.63ALINAQVVGEE336 pKa = 4.77GYY338 pKa = 10.33LHH340 pKa = 7.38AWADD344 pKa = 2.95WDD346 pKa = 3.95MNGTFDD352 pKa = 5.6ADD354 pKa = 3.61EE355 pKa = 4.79QILKK359 pKa = 10.25NYY361 pKa = 10.33NVTTGANVIPIRR373 pKa = 11.84VGDD376 pKa = 3.84DD377 pKa = 3.77AIVGNVQTRR386 pKa = 11.84FRR388 pKa = 11.84LSSSPNLPSTGYY400 pKa = 10.86AGDD403 pKa = 4.07GEE405 pKa = 4.57VEE407 pKa = 4.01DD408 pKa = 4.75RR409 pKa = 11.84VFDD412 pKa = 3.95VVDD415 pKa = 4.06PEE417 pKa = 4.51TTIQTSDD424 pKa = 3.82YY425 pKa = 8.3YY426 pKa = 10.71TAAFEE431 pKa = 5.39DD432 pKa = 3.98NWPEE436 pKa = 4.15MGDD439 pKa = 3.36FDD441 pKa = 6.53LNDD444 pKa = 2.91VVTYY448 pKa = 10.65YY449 pKa = 9.03RR450 pKa = 11.84TKK452 pKa = 10.78VISKK456 pKa = 9.88DD457 pKa = 3.63GNVLRR462 pKa = 11.84LDD464 pKa = 3.37IEE466 pKa = 4.57GTITAYY472 pKa = 10.08GASYY476 pKa = 11.43SNGLAWKK483 pKa = 9.92LDD485 pKa = 3.6GFAEE489 pKa = 4.47SDD491 pKa = 3.76VNLQLSRR498 pKa = 11.84VTKK501 pKa = 10.62NGQLRR506 pKa = 11.84NGISPFTGEE515 pKa = 4.62DD516 pKa = 3.16KK517 pKa = 11.11SVASPGGDD525 pKa = 2.84LVVVASVNLLDD536 pKa = 5.98DD537 pKa = 3.83IPIHH541 pKa = 5.96GEE543 pKa = 4.1CIYY546 pKa = 10.98HH547 pKa = 5.93RR548 pKa = 11.84TNPSCNPSLEE558 pKa = 4.19SSQMTFSVSLPFNDD572 pKa = 3.95GSEE575 pKa = 4.18PTVTSLLPLDD585 pKa = 3.85GHH587 pKa = 7.01DD588 pKa = 3.87PFIFASGEE596 pKa = 3.95GSYY599 pKa = 10.25HH600 pKa = 6.99GEE602 pKa = 3.98IFTVPPGKK610 pKa = 10.14DD611 pKa = 3.62LEE613 pKa = 4.21IHH615 pKa = 5.98TADD618 pKa = 4.54FPPTSRR624 pKa = 11.84GTLVSTFYY632 pKa = 10.79EE633 pKa = 4.28QADD636 pKa = 3.87DD637 pKa = 4.81RR638 pKa = 11.84SDD640 pKa = 3.59PSISKK645 pKa = 10.4YY646 pKa = 10.65YY647 pKa = 9.36RR648 pKa = 11.84TDD650 pKa = 3.2GNLPWGIIIPSIWNHH665 pKa = 5.04PSEE668 pKa = 4.83YY669 pKa = 9.31IDD671 pKa = 3.95VSVAYY676 pKa = 9.5PDD678 pKa = 3.6FAEE681 pKa = 4.3WAVSGGSSKK690 pKa = 9.13PTWYY694 pKa = 10.66LNPEE698 pKa = 4.28SSQTWTTSDD707 pKa = 2.96

Molecular weight: 76.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3C526|U3C526_9VIBR Inosine/xanthosine triphosphatase OS=Vibrio azureus NBRC 104587 OX=1219077 GN=VAZ01S_045_00470 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.5RR3 pKa = 11.84TFQPTVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84KK29 pKa = 9.39VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LSKK44 pKa = 10.83

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4147

0

4147

1329685

37

3512

320.6

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.997 ± 0.04

1.074 ± 0.013

5.511 ± 0.03

6.453 ± 0.038

4.226 ± 0.027

6.517 ± 0.034

2.31 ± 0.021

6.443 ± 0.032

5.632 ± 0.036

10.185 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.02

4.543 ± 0.03

3.866 ± 0.023

4.751 ± 0.036

4.452 ± 0.029

6.932 ± 0.035

5.386 ± 0.026

6.695 ± 0.029

1.238 ± 0.014

3.179 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski