Rhizopogon vesiculosus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Boletales; Suillineae; Rhizopogonaceae; Rhizopogon

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14146 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J8Q1V6|A0A1J8Q1V6_9AGAM Uncharacterized protein OS=Rhizopogon vesiculosus OX=180088 GN=AZE42_01866 PE=4 SV=1
MM1 pKa = 7.58LLALTLSILAVISVHH16 pKa = 7.19ADD18 pKa = 3.4PNPTNPGPGDD28 pKa = 3.56VFIEE32 pKa = 4.59GQSCAITWSPDD43 pKa = 2.96TTGVWKK49 pKa = 9.57TMDD52 pKa = 3.55IEE54 pKa = 5.41LKK56 pKa = 10.24TGDD59 pKa = 4.26NFNMINLTMVGTVDD73 pKa = 3.2GTDD76 pKa = 3.1ATKK79 pKa = 10.42TSFSYY84 pKa = 10.33PCPQVSPHH92 pKa = 5.94SPIYY96 pKa = 10.12FYY98 pKa = 10.88QFSSLASNTSYY109 pKa = 7.92WTGRR113 pKa = 11.84FTITDD118 pKa = 3.81SASDD122 pKa = 3.73IVPPAQSTQPDD133 pKa = 4.11GEE135 pKa = 4.6AIPWGTGQLVNGTSTTSTPVTTSTVQATDD164 pKa = 3.25AALGTPSVTSSSDD177 pKa = 3.31VNSASTSSLSSSAPAGTGTGSGLALSAQSLGWQTALTLGVSALGFTLVLL226 pKa = 3.98

Molecular weight: 23.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J8QEY4|A0A1J8QEY4_9AGAM Uncharacterized protein OS=Rhizopogon vesiculosus OX=180088 GN=AZE42_08722 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.29KK3 pKa = 10.11SRR5 pKa = 11.84STKK8 pKa = 6.49TRR10 pKa = 11.84KK11 pKa = 8.81WPLRR15 pKa = 11.84SPSLLTRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84SMTLRR30 pKa = 11.84QKK32 pKa = 10.22KK33 pKa = 9.16RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84TRR38 pKa = 11.84IKK40 pKa = 9.4GGRR43 pKa = 11.84YY44 pKa = 9.43GGG46 pKa = 3.58

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14140

6

14146

5198710

8

5048

367.5

40.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.114 ± 0.018

1.321 ± 0.009

5.572 ± 0.015

5.647 ± 0.019

3.747 ± 0.014

6.098 ± 0.019

2.702 ± 0.01

5.097 ± 0.017

4.342 ± 0.018

9.256 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.213 ± 0.008

3.386 ± 0.011

6.381 ± 0.024

3.767 ± 0.015

6.04 ± 0.019

9.007 ± 0.027

6.042 ± 0.013

6.353 ± 0.015

1.397 ± 0.008

2.653 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski