Keratinibaculum paraultunense
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2180 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R3KTN9|A0A4R3KTN9_9FIRM Uncharacterized protein YutE (UPF0331/DUF86 family) OS=Keratinibaculum paraultunense OX=1278232 GN=EDD65_11280 PE=3 SV=1
MM1 pKa = 7.26 TLINTIYY8 pKa = 10.29 FYY10 pKa = 11.04 DD11 pKa = 3.46 EE12 pKa = 4.02 WVDD15 pKa = 3.57 SFNVKK20 pKa = 8.16 NTIEE24 pKa = 4.16 DD25 pKa = 3.56 EE26 pKa = 4.58 FYY28 pKa = 10.95 LADD31 pKa = 4.15 GSTVKK36 pKa = 10.6 SDD38 pKa = 3.87 FMNMTYY44 pKa = 10.68 GSHH47 pKa = 6.31 SFVGVDD53 pKa = 4.15 GYY55 pKa = 9.17 TVSYY59 pKa = 11.07 LNLKK63 pKa = 10.13 NSSQMVFILPDD74 pKa = 3.68 EE75 pKa = 4.64 GVSPYY80 pKa = 10.78 DD81 pKa = 3.73 IISDD85 pKa = 3.95 PEE87 pKa = 4.27 LLDD90 pKa = 3.62 EE91 pKa = 5.07 ALNSLSTDD99 pKa = 3.12 EE100 pKa = 4.17 MQMGEE105 pKa = 4.77 VIFKK109 pKa = 10.31 IPKK112 pKa = 8.45 LTFLQVLSS120 pKa = 4.07
Molecular weight: 13.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.872
Patrickios 0.846
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A4R3KZ24|A0A4R3KZ24_9FIRM UPF0342 protein EDD65_102230 OS=Keratinibaculum paraultunense OX=1278232 GN=EDD65_102230 PE=3 SV=1
MM1 pKa = 7.42 NNNNGFKK8 pKa = 10.42 SGALMGAILGTSVGMFFGARR28 pKa = 11.84 MNPIQKK34 pKa = 10.22 RR35 pKa = 11.84 KK36 pKa = 6.47 ITKK39 pKa = 9.62 NIRR42 pKa = 11.84 KK43 pKa = 9.22 ARR45 pKa = 11.84 STLMNGVNSLLRR57 pKa = 4.55
Molecular weight: 6.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.115
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.193
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2180
0
2180
661144
25
2698
303.3
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.134 ± 0.047
0.886 ± 0.017
5.863 ± 0.044
8.155 ± 0.06
4.17 ± 0.042
6.535 ± 0.047
1.399 ± 0.016
10.845 ± 0.067
9.184 ± 0.056
9.161 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.619 ± 0.026
5.915 ± 0.046
3.073 ± 0.027
2.271 ± 0.024
3.673 ± 0.035
5.369 ± 0.036
4.641 ± 0.034
6.154 ± 0.037
0.642 ± 0.016
4.313 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here