Halococcus morrhuae DSM 1307
Average proteome isoelectric point is 4.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3035 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M0MW88|M0MW88_HALMO Fe/B12 periplasmic-binding domain-containing protein OS=Halococcus morrhuae DSM 1307 OX=931277 GN=C448_02528 PE=4 SV=1
MM1 pKa = 7.31 SAPWTEE7 pKa = 3.4 WDD9 pKa = 4.58 HH10 pKa = 6.32 IVKK13 pKa = 9.86 IDD15 pKa = 3.8 PDD17 pKa = 3.54 KK18 pKa = 11.54 SLVDD22 pKa = 3.55 GEE24 pKa = 4.76 SYY26 pKa = 11.42 EE27 pKa = 4.38 DD28 pKa = 3.58 VCRR31 pKa = 11.84 TGTDD35 pKa = 3.47 AIEE38 pKa = 4.23 VGGTTGMTEE47 pKa = 4.14 EE48 pKa = 4.21 KK49 pKa = 9.2 MQAVVEE55 pKa = 4.4 ACSEE59 pKa = 4.21 HH60 pKa = 7.46 DD61 pKa = 3.56 VPLYY65 pKa = 10.41 IEE67 pKa = 4.88 PGVDD71 pKa = 2.76 ATVVHH76 pKa = 6.68 TDD78 pKa = 3.08 SLDD81 pKa = 3.54 GYY83 pKa = 9.86 LIPIVFNAGDD93 pKa = 3.95 VSWMTGAHH101 pKa = 6.09 KK102 pKa = 10.18 EE103 pKa = 3.94 WVRR106 pKa = 11.84 IDD108 pKa = 5.87 DD109 pKa = 4.98 GIDD112 pKa = 3.04 WARR115 pKa = 11.84 TNTEE119 pKa = 3.76 AYY121 pKa = 9.34 IVLNPDD127 pKa = 3.12 SSVATYY133 pKa = 8.36 TQANCDD139 pKa = 3.55 LDD141 pKa = 5.83 AEE143 pKa = 4.56 DD144 pKa = 3.49 VAAYY148 pKa = 8.22 ATIAEE153 pKa = 4.22 RR154 pKa = 11.84 MFGQEE159 pKa = 3.33 IVYY162 pKa = 10.08 VEE164 pKa = 3.9 YY165 pKa = 10.91 SGTFGDD171 pKa = 4.2 PEE173 pKa = 3.96 IVAAAGDD180 pKa = 4.13 ALDD183 pKa = 4.47 EE184 pKa = 4.35 ATLFYY189 pKa = 11.1 GGGIHH194 pKa = 7.71 DD195 pKa = 4.55 YY196 pKa = 11.32 DD197 pKa = 4.04 SAHH200 pKa = 6.36 EE201 pKa = 4.01 MGEE204 pKa = 4.0 RR205 pKa = 11.84 ADD207 pKa = 3.9 TVVVGDD213 pKa = 4.2 LVHH216 pKa = 7.49 DD217 pKa = 4.38 EE218 pKa = 4.4 GVDD221 pKa = 3.56 AVRR224 pKa = 11.84 EE225 pKa = 4.32 TVRR228 pKa = 11.84 GATDD232 pKa = 3.44 ASS234 pKa = 3.68
Molecular weight: 25.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.834
IPC_protein 3.846
Toseland 3.63
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.668
Grimsley 3.541
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.164
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.961
Patrickios 1.252
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|M0N5B9|M0N5B9_HALMO Chlorite dismutase OS=Halococcus morrhuae DSM 1307 OX=931277 GN=C448_00075 PE=4 SV=1
MM1 pKa = 7.89 RR2 pKa = 11.84 SNSVGWSSARR12 pKa = 11.84 TRR14 pKa = 11.84 ISVVQIRR21 pKa = 11.84 RR22 pKa = 11.84 LIWKK26 pKa = 9.2 IIQGFSRR33 pKa = 11.84 VIQQRR38 pKa = 11.84 XSSARR43 pKa = 11.84 TRR45 pKa = 11.84 ISVVQIRR52 pKa = 11.84 RR53 pKa = 11.84 LIWKK57 pKa = 9.2 IIQGFSRR64 pKa = 11.84 VIQQRR69 pKa = 11.84 LQEE72 pKa = 4.01 QQ73 pKa = 3.38
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.452
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.31
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.047
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.132
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3035
0
3035
843377
27
1867
277.9
30.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.031 ± 0.062
0.743 ± 0.013
8.066 ± 0.053
8.398 ± 0.069
3.424 ± 0.032
8.628 ± 0.048
2.071 ± 0.024
4.45 ± 0.033
1.838 ± 0.025
9.14 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.81 ± 0.018
2.423 ± 0.024
4.554 ± 0.027
2.446 ± 0.022
6.579 ± 0.037
5.602 ± 0.038
6.513 ± 0.039
8.504 ± 0.046
1.122 ± 0.018
2.645 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here