Acinetobacter rudis CIP 110305
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3609 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S3NHV0|S3NHV0_9GAMM 3-oxoadipate enol-lactonase OS=Acinetobacter rudis CIP 110305 OX=421052 GN=F945_01808 PE=4 SV=1
MM1 pKa = 7.56 VNLQMEE7 pKa = 4.72 GFEE10 pKa = 4.26 VVLWILGVLFGSVVIIGSVLLSTAIYY36 pKa = 8.43 TKK38 pKa = 10.09 RR39 pKa = 11.84 LKK41 pKa = 10.52 IIDD44 pKa = 3.83 QQATASEE51 pKa = 4.17 DD52 pKa = 3.97 RR53 pKa = 11.84 YY54 pKa = 11.53 NSMPWHH60 pKa = 6.07 VMNGDD65 pKa = 3.36 QDD67 pKa = 3.85 VSSSQDD73 pKa = 3.11 SYY75 pKa = 11.82 SSCDD79 pKa = 3.55 TQSSDD84 pKa = 4.79 SSSCDD89 pKa = 3.15 SSSSSDD95 pKa = 3.01
Molecular weight: 10.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.923
IPC_protein 3.859
Toseland 3.643
ProMoST 3.961
Dawson 3.872
Bjellqvist 4.101
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.916
Protein with the highest isoelectric point:
>tr|S3MQ38|S3MQ38_9GAMM Pantothenate synthetase OS=Acinetobacter rudis CIP 110305 OX=421052 GN=panC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3609
0
3609
1134487
26
2311
314.3
35.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.69 ± 0.051
1.006 ± 0.012
5.125 ± 0.031
5.6 ± 0.039
4.269 ± 0.029
6.337 ± 0.038
2.451 ± 0.022
6.976 ± 0.036
5.392 ± 0.034
10.607 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.477 ± 0.02
4.441 ± 0.035
3.772 ± 0.024
5.839 ± 0.044
4.285 ± 0.03
6.381 ± 0.029
5.152 ± 0.026
6.542 ± 0.036
1.276 ± 0.015
3.383 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here