Amino acid dipepetide frequency for Novosphingobium tardaugens NBRC 16725

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.163AlaAla: 17.163 ± 0.179	1.093AlaCys: 1.093 ± 0.034	7.504AlaAsp: 7.504 ± 0.089	7.428AlaGlu: 7.428 ± 0.099	4.355AlaPhe: 4.355 ± 0.066	11.048AlaGly: 11.048 ± 0.144	2.417AlaHis: 2.417 ± 0.047	6.934AlaIle: 6.934 ± 0.078	3.786AlaLys: 3.786 ± 0.067	13.422AlaLeu: 13.422 ± 0.141	3.85AlaMet: 3.85 ± 0.058	3.319AlaAsn: 3.319 ± 0.061	5.804AlaPro: 5.804 ± 0.085	4.877AlaGln: 4.877 ± 0.077	9.275AlaArg: 9.275 ± 0.108	6.493AlaSer: 6.493 ± 0.084	6.31AlaThr: 6.31 ± 0.09	8.295AlaVal: 8.295 ± 0.09	1.634AlaTrp: 1.634 ± 0.041	2.761AlaTyr: 2.761 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.086CysAla: 1.086 ± 0.031	0.098CysCys: 0.098 ± 0.009	0.614CysAsp: 0.614 ± 0.023	0.434CysGlu: 0.434 ± 0.017	0.308CysPhe: 0.308 ± 0.018	0.912CysGly: 0.912 ± 0.028	0.229CysHis: 0.229 ± 0.013	0.415CysIle: 0.415 ± 0.018	0.186CysLys: 0.186 ± 0.012	0.743CysLeu: 0.743 ± 0.027	0.156CysMet: 0.156 ± 0.011	0.247CysAsn: 0.247 ± 0.012	0.507CysPro: 0.507 ± 0.025	0.228CysGln: 0.228 ± 0.014	0.566CysArg: 0.566 ± 0.019	0.453CysSer: 0.453 ± 0.018	0.435CysThr: 0.435 ± 0.022	0.588CysVal: 0.588 ± 0.024	0.113CysTrp: 0.113 ± 0.01	0.192CysTyr: 0.192 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.702AspAla: 7.702 ± 0.093	0.536AspCys: 0.536 ± 0.022	3.481AspAsp: 3.481 ± 0.058	3.214AspGlu: 3.214 ± 0.056	2.222AspPhe: 2.222 ± 0.04	5.722AspGly: 5.722 ± 0.087	1.496AspHis: 1.496 ± 0.037	3.169AspIle: 3.169 ± 0.051	1.779AspLys: 1.779 ± 0.036	5.808AspLeu: 5.808 ± 0.072	1.532AspMet: 1.532 ± 0.035	1.612AspAsn: 1.612 ± 0.041	3.667AspPro: 3.667 ± 0.06	1.838AspGln: 1.838 ± 0.037	4.677AspArg: 4.677 ± 0.064	2.511AspSer: 2.511 ± 0.043	2.757AspThr: 2.757 ± 0.052	4.206AspVal: 4.206 ± 0.057	1.111AspTrp: 1.111 ± 0.031	1.725AspTyr: 1.725 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	7.067GluAla: 7.067 ± 0.086	0.356GluCys: 0.356 ± 0.017	2.705GluAsp: 2.705 ± 0.052	3.063GluGlu: 3.063 ± 0.062	1.633GluPhe: 1.633 ± 0.039	4.394GluGly: 4.394 ± 0.059	1.159GluHis: 1.159 ± 0.034	2.956GluIle: 2.956 ± 0.044	1.954GluLys: 1.954 ± 0.042	5.198GluLeu: 5.198 ± 0.08	1.448GluMet: 1.448 ± 0.035	1.6GluAsn: 1.6 ± 0.034	2.533GluPro: 2.533 ± 0.047	2.372GluGln: 2.372 ± 0.044	4.683GluArg: 4.683 ± 0.069	2.397GluSer: 2.397 ± 0.043	3.126GluThr: 3.126 ± 0.063	3.353GluVal: 3.353 ± 0.066	0.851GluTrp: 0.851 ± 0.027	1.069GluTyr: 1.069 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.804PheAla: 4.804 ± 0.064	0.36PheCys: 0.36 ± 0.016	2.674PheAsp: 2.674 ± 0.044	1.999PheGlu: 1.999 ± 0.038	1.227PhePhe: 1.227 ± 0.03	3.727PheGly: 3.727 ± 0.064	0.826PheHis: 0.826 ± 0.026	1.468PheIle: 1.468 ± 0.04	0.886PheLys: 0.886 ± 0.028	3.041PheLeu: 3.041 ± 0.056	0.832PheMet: 0.832 ± 0.024	1.128PheAsn: 1.128 ± 0.037	1.542PhePro: 1.542 ± 0.037	0.921PheGln: 0.921 ± 0.026	2.229PheArg: 2.229 ± 0.044	2.191PheSer: 2.191 ± 0.043	2.117PheThr: 2.117 ± 0.04	2.603PheVal: 2.603 ± 0.052	0.502PheTrp: 0.502 ± 0.024	0.916PheTyr: 0.916 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	9.911GlyAla: 9.911 ± 0.127	0.813GlyCys: 0.813 ± 0.026	5.008GlyAsp: 5.008 ± 0.089	4.661GlyGlu: 4.661 ± 0.069	3.62GlyPhe: 3.62 ± 0.057	8.619GlyGly: 8.619 ± 0.354	1.935GlyHis: 1.935 ± 0.043	4.716GlyIle: 4.716 ± 0.072	3.559GlyLys: 3.559 ± 0.06	8.557GlyLeu: 8.557 ± 0.09	2.468GlyMet: 2.468 ± 0.049	2.592GlyAsn: 2.592 ± 0.077	3.695GlyPro: 3.695 ± 0.052	3.233GlyGln: 3.233 ± 0.058	5.963GlyArg: 5.963 ± 0.078	4.89GlySer: 4.89 ± 0.097	5.066GlyThr: 5.066 ± 0.094	6.119GlyVal: 6.119 ± 0.072	1.518GlyTrp: 1.518 ± 0.037	2.522GlyTyr: 2.522 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.562HisAla: 2.562 ± 0.052	0.269HisCys: 0.269 ± 0.016	1.339HisAsp: 1.339 ± 0.031	1.014HisGlu: 1.014 ± 0.027	0.905HisPhe: 0.905 ± 0.027	2.034HisGly: 2.034 ± 0.051	0.572HisHis: 0.572 ± 0.023	1.036HisIle: 1.036 ± 0.029	0.534HisLys: 0.534 ± 0.024	1.996HisLeu: 1.996 ± 0.04	0.501HisMet: 0.501 ± 0.022	0.53HisAsn: 0.53 ± 0.02	1.323HisPro: 1.323 ± 0.035	0.571HisGln: 0.571 ± 0.021	1.46HisArg: 1.46 ± 0.04	1.001HisSer: 1.001 ± 0.03	0.893HisThr: 0.893 ± 0.028	1.539HisVal: 1.539 ± 0.038	0.374HisTrp: 0.374 ± 0.018	0.612HisTyr: 0.612 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	7.965IleAla: 7.965 ± 0.082	0.479IleCys: 0.479 ± 0.021	3.824IleAsp: 3.824 ± 0.06	3.293IleGlu: 3.293 ± 0.057	1.535IlePhe: 1.535 ± 0.038	5.096IleGly: 5.096 ± 0.074	0.943IleHis: 0.943 ± 0.03	2.124IleIle: 2.124 ± 0.043	1.229IleLys: 1.229 ± 0.032	4.021IleLeu: 4.021 ± 0.058	0.999IleMet: 0.999 ± 0.031	1.517IleAsn: 1.517 ± 0.036	2.475IlePro: 2.475 ± 0.05	1.269IleGln: 1.269 ± 0.032	3.301IleArg: 3.301 ± 0.049	2.81IleSer: 2.81 ± 0.054	2.873IleThr: 2.873 ± 0.051	4.082IleVal: 4.082 ± 0.063	0.593IleTrp: 0.593 ± 0.025	1.139IleTyr: 1.139 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.963LysAla: 3.963 ± 0.072	0.16LysCys: 0.16 ± 0.011	1.568LysAsp: 1.568 ± 0.035	1.359LysGlu: 1.359 ± 0.036	0.834LysPhe: 0.834 ± 0.027	2.648LysGly: 2.648 ± 0.05	0.588LysHis: 0.588 ± 0.024	1.495LysIle: 1.495 ± 0.044	1.103LysLys: 1.103 ± 0.033	3.023LysLeu: 3.023 ± 0.047	0.72LysMet: 0.72 ± 0.023	0.776LysAsn: 0.776 ± 0.027	1.88LysPro: 1.88 ± 0.044	1.041LysGln: 1.041 ± 0.032	2.157LysArg: 2.157 ± 0.047	1.613LysSer: 1.613 ± 0.032	1.673LysThr: 1.673 ± 0.04	2.277LysVal: 2.277 ± 0.046	0.374LysTrp: 0.374 ± 0.017	0.641LysTyr: 0.641 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	13.761LeuAla: 13.761 ± 0.146	0.881LeuCys: 0.881 ± 0.027	6.254LeuAsp: 6.254 ± 0.083	4.967LeuGlu: 4.967 ± 0.076	3.563LeuPhe: 3.563 ± 0.063	8.241LeuGly: 8.241 ± 0.081	1.923LeuHis: 1.923 ± 0.045	4.801LeuIle: 4.801 ± 0.083	2.804LeuLys: 2.804 ± 0.049	9.069LeuLeu: 9.069 ± 0.134	2.11LeuMet: 2.11 ± 0.044	2.507LeuAsn: 2.507 ± 0.044	5.446LeuPro: 5.446 ± 0.078	2.73LeuGln: 2.73 ± 0.052	6.712LeuArg: 6.712 ± 0.087	5.903LeuSer: 5.903 ± 0.073	5.621LeuThr: 5.621 ± 0.078	7.026LeuVal: 7.026 ± 0.08	1.186LeuTrp: 1.186 ± 0.035	1.993LeuTyr: 1.993 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.309MetAla: 3.309 ± 0.06	0.173MetCys: 0.173 ± 0.011	1.213MetAsp: 1.213 ± 0.031	1.182MetGlu: 1.182 ± 0.029	0.745MetPhe: 0.745 ± 0.025	2.047MetGly: 2.047 ± 0.039	0.516MetHis: 0.516 ± 0.024	1.374MetIle: 1.374 ± 0.032	0.866MetLys: 0.866 ± 0.027	2.602MetLeu: 2.602 ± 0.049	0.64MetMet: 0.64 ± 0.022	0.756MetAsn: 0.756 ± 0.025	1.57MetPro: 1.57 ± 0.037	0.858MetGln: 0.858 ± 0.025	1.842MetArg: 1.842 ± 0.041	1.337MetSer: 1.337 ± 0.035	1.712MetThr: 1.712 ± 0.038	1.667MetVal: 1.667 ± 0.04	0.217MetTrp: 0.217 ± 0.014	0.3MetTyr: 0.3 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.564AsnAla: 3.564 ± 0.063	0.258AsnCys: 0.258 ± 0.015	1.603AsnAsp: 1.603 ± 0.037	1.184AsnGlu: 1.184 ± 0.033	1.009AsnPhe: 1.009 ± 0.029	2.757AsnGly: 2.757 ± 0.073	0.543AsnHis: 0.543 ± 0.019	1.463AsnIle: 1.463 ± 0.04	0.681AsnLys: 0.681 ± 0.027	2.631AsnLeu: 2.631 ± 0.046	0.558AsnMet: 0.558 ± 0.021	0.834AsnAsn: 0.834 ± 0.035	1.959AsnPro: 1.959 ± 0.041	0.797AsnGln: 0.797 ± 0.027	2.034AsnArg: 2.034 ± 0.043	1.321AsnSer: 1.321 ± 0.041	1.357AsnThr: 1.357 ± 0.052	2.053AsnVal: 2.053 ± 0.049	0.503AsnTrp: 0.503 ± 0.019	0.768AsnTyr: 0.768 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	6.671ProAla: 6.671 ± 0.088	0.351ProCys: 0.351 ± 0.018	4.017ProAsp: 4.017 ± 0.066	3.579ProGlu: 3.579 ± 0.063	1.898ProPhe: 1.898 ± 0.039	4.765ProGly: 4.765 ± 0.066	1.11ProHis: 1.11 ± 0.035	2.389ProIle: 2.389 ± 0.044	1.385ProLys: 1.385 ± 0.034	4.897ProLeu: 4.897 ± 0.07	1.258ProMet: 1.258 ± 0.031	1.334ProAsn: 1.334 ± 0.034	2.626ProPro: 2.626 ± 0.066	1.866ProGln: 1.866 ± 0.034	2.895ProArg: 2.895 ± 0.052	2.581ProSer: 2.581 ± 0.047	2.41ProThr: 2.41 ± 0.044	4.277ProVal: 4.277 ± 0.061	0.684ProTrp: 0.684 ± 0.023	1.108ProTyr: 1.108 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.472GlnAla: 4.472 ± 0.067	0.265GlnCys: 0.265 ± 0.013	1.656GlnAsp: 1.656 ± 0.038	1.543GlnGlu: 1.543 ± 0.038	1.298GlnPhe: 1.298 ± 0.033	2.755GlnGly: 2.755 ± 0.052	0.695GlnHis: 0.695 ± 0.024	1.918GlnIle: 1.918 ± 0.043	1.03GlnLys: 1.03 ± 0.032	3.165GlnLeu: 3.165 ± 0.051	0.887GlnMet: 0.887 ± 0.025	0.895GlnAsn: 0.895 ± 0.031	1.835GlnPro: 1.835 ± 0.035	1.3GlnGln: 1.3 ± 0.037	2.519GlnArg: 2.519 ± 0.05	1.81GlnSer: 1.81 ± 0.059	1.76GlnThr: 1.76 ± 0.042	2.382GlnVal: 2.382 ± 0.046	0.533GlnTrp: 0.533 ± 0.019	0.73GlnTyr: 0.73 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	7.972ArgAla: 7.972 ± 0.091	0.551ArgCys: 0.551 ± 0.023	4.343ArgAsp: 4.343 ± 0.07	3.951ArgGlu: 3.951 ± 0.06	3.088ArgPhe: 3.088 ± 0.054	4.89ArgGly: 4.89 ± 0.067	1.701ArgHis: 1.701 ± 0.038	4.163ArgIle: 4.163 ± 0.059	2.329ArgLys: 2.329 ± 0.046	7.659ArgLeu: 7.659 ± 0.102	1.93ArgMet: 1.93 ± 0.044	2.084ArgAsn: 2.084 ± 0.043	3.391ArgPro: 3.391 ± 0.059	2.646ArgGln: 2.646 ± 0.049	5.261ArgArg: 5.261 ± 0.079	3.528ArgSer: 3.528 ± 0.053	3.388ArgThr: 3.388 ± 0.056	4.622ArgVal: 4.622 ± 0.064	1.172ArgTrp: 1.172 ± 0.031	1.966ArgTyr: 1.966 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.373SerAla: 6.373 ± 0.082	0.43SerCys: 0.43 ± 0.018	3.175SerAsp: 3.175 ± 0.052	2.592SerGlu: 2.592 ± 0.046	2.114SerPhe: 2.114 ± 0.042	5.636SerGly: 5.636 ± 0.103	1.071SerHis: 1.071 ± 0.027	2.605SerIle: 2.605 ± 0.059	1.403SerLys: 1.403 ± 0.034	5.236SerLeu: 5.236 ± 0.067	1.227SerMet: 1.227 ± 0.033	1.388SerAsn: 1.388 ± 0.038	2.796SerPro: 2.796 ± 0.05	1.712SerGln: 1.712 ± 0.04	3.421SerArg: 3.421 ± 0.055	2.842SerSer: 2.842 ± 0.052	2.696SerThr: 2.696 ± 0.059	3.712SerVal: 3.712 ± 0.067	0.781SerTrp: 0.781 ± 0.026	1.479SerTyr: 1.479 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	6.322ThrAla: 6.322 ± 0.08	0.423ThrCys: 0.423 ± 0.019	2.993ThrAsp: 2.993 ± 0.056	2.361ThrGlu: 2.361 ± 0.047	1.877ThrPhe: 1.877 ± 0.045	5.563ThrGly: 5.563 ± 0.088	1.037ThrHis: 1.037 ± 0.028	3.091ThrIle: 3.091 ± 0.058	1.283ThrLys: 1.283 ± 0.037	5.696ThrLeu: 5.696 ± 0.067	1.245ThrMet: 1.245 ± 0.031	1.443ThrAsn: 1.443 ± 0.041	3.373ThrPro: 3.373 ± 0.057	1.552ThrGln: 1.552 ± 0.036	3.519ThrArg: 3.519 ± 0.047	2.765ThrSer: 2.765 ± 0.053	2.653ThrThr: 2.653 ± 0.066	4.239ThrVal: 4.239 ± 0.075	0.679ThrTrp: 0.679 ± 0.026	1.255ThrTyr: 1.255 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	8.897ValAla: 8.897 ± 0.095	0.597ValCys: 0.597 ± 0.021	4.34ValAsp: 4.34 ± 0.066	4.247ValGlu: 4.247 ± 0.065	2.251ValPhe: 2.251 ± 0.04	5.401ValGly: 5.401 ± 0.079	1.371ValHis: 1.371 ± 0.03	3.79ValIle: 3.79 ± 0.059	1.967ValLys: 1.967 ± 0.043	6.759ValLeu: 6.759 ± 0.08	1.755ValMet: 1.755 ± 0.041	2.094ValAsn: 2.094 ± 0.047	3.882ValPro: 3.882 ± 0.054	2.22ValGln: 2.22 ± 0.047	4.873ValArg: 4.873 ± 0.065	4.148ValSer: 4.148 ± 0.061	4.508ValThr: 4.508 ± 0.072	5.179ValVal: 5.179 ± 0.087	0.89ValTrp: 0.89 ± 0.028	1.414ValTyr: 1.414 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.354TrpAla: 1.354 ± 0.039	0.136TrpCys: 0.136 ± 0.011	0.806TrpAsp: 0.806 ± 0.026	0.639TrpGlu: 0.639 ± 0.023	0.581TrpPhe: 0.581 ± 0.023	0.998TrpGly: 0.998 ± 0.029	0.395TrpHis: 0.395 ± 0.019	0.712TrpIle: 0.712 ± 0.024	0.461TrpLys: 0.461 ± 0.016	1.76TrpLeu: 1.76 ± 0.047	0.364TrpMet: 0.364 ± 0.017	0.505TrpAsn: 0.505 ± 0.022	0.707TrpPro: 0.707 ± 0.026	0.64TrpGln: 0.64 ± 0.023	1.29TrpArg: 1.29 ± 0.032	0.839TrpSer: 0.839 ± 0.025	0.815TrpThr: 0.815 ± 0.027	0.807TrpVal: 0.807 ± 0.028	0.218TrpTrp: 0.218 ± 0.013	0.295TrpTyr: 0.295 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.852TyrAla: 2.852 ± 0.053	0.282TyrCys: 0.282 ± 0.015	1.648TyrAsp: 1.648 ± 0.064	1.204TyrGlu: 1.204 ± 0.033	0.931TyrPhe: 0.931 ± 0.027	2.212TyrGly: 2.212 ± 0.043	0.539TyrHis: 0.539 ± 0.022	0.946TyrIle: 0.946 ± 0.028	0.669TyrLys: 0.669 ± 0.028	2.213TyrLeu: 2.213 ± 0.043	0.445TyrMet: 0.445 ± 0.018	0.715TyrAsn: 0.715 ± 0.031	1.09TyrPro: 1.09 ± 0.032	0.782TyrGln: 0.782 ± 0.025	1.957TyrArg: 1.957 ± 0.043	1.256TyrSer: 1.256 ± 0.034	1.199TyrThr: 1.199 ± 0.038	1.558TyrVal: 1.558 ± 0.037	0.387TyrTrp: 0.387 ± 0.02	0.712TyrTyr: 0.712 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4005 proteins (1284881 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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