Mustela putorius papillomavirus 1
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T1YE43|T1YE43_9PAPI Minor capsid protein L2 OS=Mustela putorius papillomavirus 1 OX=2259540 GN=L2 PE=3 SV=1
MM1 pKa = 7.58 PASTPPRR8 pKa = 11.84 GTGPFYY14 pKa = 10.54 PLLLDD19 pKa = 3.81 VVAEE23 pKa = 4.13 AGGPPEE29 pKa = 4.51 EE30 pKa = 5.12 DD31 pKa = 3.79 EE32 pKa = 4.8 EE33 pKa = 4.33 PAAPVGPHH41 pKa = 6.5 PVPPPIPPPPPPPLPPMPPINEE63 pKa = 4.18 GEE65 pKa = 3.99 EE66 pKa = 4.45 DD67 pKa = 3.62 EE68 pKa = 5.88 DD69 pKa = 4.93 EE70 pKa = 4.28 EE71 pKa = 5.47 DD72 pKa = 4.72 EE73 pKa = 4.26 EE74 pKa = 5.87 DD75 pKa = 3.86 EE76 pKa = 4.81 EE77 pKa = 4.59 EE78 pKa = 4.76 DD79 pKa = 5.13 SEE81 pKa = 5.07 TEE83 pKa = 4.38 DD84 pKa = 3.45 EE85 pKa = 4.5 SQEE88 pKa = 3.85 GRR90 pKa = 11.84 HH91 pKa = 6.15 PDD93 pKa = 3.27 SLLASLMQTWGDD105 pKa = 3.75 DD106 pKa = 3.92 LDD108 pKa = 4.26 RR109 pKa = 11.84 LQDD112 pKa = 4.03 KK113 pKa = 11.07 INGEE117 pKa = 4.06 LDD119 pKa = 2.95 KK120 pKa = 11.27 LRR122 pKa = 11.84 RR123 pKa = 11.84 TVGLPPRR130 pKa = 11.84 SPPSS134 pKa = 3.25
Molecular weight: 14.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.291
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.579
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.63
Rodwell 3.605
Grimsley 3.49
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.617
EMBOSS 3.643
Sillero 3.884
Patrickios 3.656
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.836
Protein with the highest isoelectric point:
>tr|T1YEN9|T1YEN9_9PAPI Major capsid protein L1 OS=Mustela putorius papillomavirus 1 OX=2259540 GN=L1 PE=3 SV=1
MM1 pKa = 7.46 IVGNNYY7 pKa = 9.41 CVYY10 pKa = 11.11 VLTCTKK16 pKa = 9.82 KK17 pKa = 8.25 GTYY20 pKa = 9.13 IRR22 pKa = 11.84 SCKK25 pKa = 9.73 EE26 pKa = 3.44 VTLIAHH32 pKa = 6.07 MAKK35 pKa = 8.59 PTTVLGLSRR44 pKa = 11.84 RR45 pKa = 11.84 LQQPVEE51 pKa = 4.43 RR52 pKa = 11.84 ILIKK56 pKa = 10.7 CIFCEE61 pKa = 4.95 LPLTNLDD68 pKa = 3.44 KK69 pKa = 11.62 QEE71 pKa = 4.09 FDD73 pKa = 3.48 KK74 pKa = 11.37 KK75 pKa = 10.87 ALNLVWYY82 pKa = 9.27 GNFVFGVCSYY92 pKa = 11.09 CCYY95 pKa = 10.39 KK96 pKa = 10.64 KK97 pKa = 10.86 SFEE100 pKa = 4.4 DD101 pKa = 3.99 CVKK104 pKa = 9.7 NTAICLEE111 pKa = 4.21 GDD113 pKa = 3.44 GVEE116 pKa = 5.32 AITNVPLQRR125 pKa = 11.84 LVVRR129 pKa = 11.84 CQHH132 pKa = 5.93 CMSVLSFLEE141 pKa = 3.77 KK142 pKa = 10.57 LNCVVRR148 pKa = 11.84 KK149 pKa = 10.04 SPFILVRR156 pKa = 11.84 TCWRR160 pKa = 11.84 NKK162 pKa = 9.99 CNSCFRR168 pKa = 11.84 NDD170 pKa = 2.82 WW171 pKa = 3.42
Molecular weight: 19.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.149
IPC2_protein 8.024
IPC_protein 7.805
Toseland 7.234
ProMoST 8.419
Dawson 8.521
Bjellqvist 9.019
Wikipedia 8.434
Rodwell 8.551
Grimsley 7.161
Solomon 8.595
Lehninger 8.609
Nozaki 9.355
DTASelect 8.653
Thurlkill 8.712
EMBOSS 8.726
Sillero 9.092
Patrickios 3.643
IPC_peptide 8.58
IPC2_peptide 8.331
IPC2.peptide.svr19 8.466
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2753
97
595
344.1
38.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.504 ± 0.456
2.761 ± 0.652
5.194 ± 0.571
7.011 ± 0.643
4.686 ± 0.666
8.681 ± 1.525
1.562 ± 0.185
4.395 ± 0.756
4.904 ± 0.727
7.991 ± 0.839
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.671 ± 0.35
4.831 ± 0.651
6.538 ± 1.189
3.959 ± 0.526
5.739 ± 0.704
8.209 ± 0.91
6.684 ± 0.706
6.175 ± 0.663
1.562 ± 0.487
2.942 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here