Hephaestia caeni

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Hephaestia

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A397P5N3|A0A397P5N3_9SPHN Two-component system response regulator RegA OS=Hephaestia caeni OX=645617 GN=DFR49_1775 PE=4 SV=1
MM1 pKa = 7.26VPLPGQEE8 pKa = 4.17PYY10 pKa = 10.86IMPLTTAQSFAWNLAKK26 pKa = 9.9TLMTIIVLIEE36 pKa = 3.66TDD38 pKa = 3.08RR39 pKa = 11.84GYY41 pKa = 11.62VAMPSDD47 pKa = 4.56EE48 pKa = 4.67FDD50 pKa = 3.95GDD52 pKa = 3.76PDD54 pKa = 5.3RR55 pKa = 11.84IICEE59 pKa = 3.93YY60 pKa = 11.08DD61 pKa = 2.74PWSS64 pKa = 3.41

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A397NH55|A0A397NH55_9SPHN Translation initiation factor IF-1 OS=Hephaestia caeni OX=645617 GN=infA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 9.14VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4251

0

4251

1356717

27

4338

319.2

34.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.834 ± 0.055

0.738 ± 0.011

6.269 ± 0.029

5.14 ± 0.037

3.468 ± 0.026

8.837 ± 0.044

2.051 ± 0.017

5.123 ± 0.024

2.736 ± 0.027

9.756 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.017

2.427 ± 0.027

5.466 ± 0.028

2.952 ± 0.023

7.601 ± 0.042

5.018 ± 0.028

5.527 ± 0.031

7.105 ± 0.029

1.448 ± 0.015

2.226 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski