Sphingosinithalassobacter sp. zrk23

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingosinithalassobacter; unclassified Sphingosinithalassobacter

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6Y8L0|A0A6G6Y8L0_9SPHN Type IV secretion system protein TraC OS=Sphingosinithalassobacter sp. zrk23 OX=2711215 GN=traC PE=4 SV=1
MM1 pKa = 7.6ASIKK5 pKa = 10.21RR6 pKa = 11.84LSLILMTTCAGAALSACDD24 pKa = 3.8GASDD28 pKa = 3.59IASPGTGGNVIINNPAPTPTPTPTPTPTASLVTPATGCPTIADD71 pKa = 4.04PQGLTDD77 pKa = 5.31DD78 pKa = 4.27GTITGPTGEE87 pKa = 4.42YY88 pKa = 9.49RR89 pKa = 11.84VCTLPDD95 pKa = 4.81RR96 pKa = 11.84INTSINLPQIDD107 pKa = 3.99GLLYY111 pKa = 10.46RR112 pKa = 11.84LGGRR116 pKa = 11.84VDD118 pKa = 3.2VGTDD122 pKa = 3.08QGATSTGTDD131 pKa = 3.08VTLSIEE137 pKa = 3.97PGVIIFGGTGVSWLAVNRR155 pKa = 11.84GNAIDD160 pKa = 4.62AVGTPTDD167 pKa = 4.55PIIFTSRR174 pKa = 11.84DD175 pKa = 3.21NVLGLNTDD183 pKa = 3.28SSSGQWGGVVLMGRR197 pKa = 11.84APITDD202 pKa = 3.72CTVTPGATPGTAACEE217 pKa = 4.09RR218 pKa = 11.84QTEE221 pKa = 4.47GAVDD225 pKa = 3.41PAVYY229 pKa = 10.27GGANTADD236 pKa = 3.41SSGTMQYY243 pKa = 10.23VQIRR247 pKa = 11.84YY248 pKa = 9.15SGYY251 pKa = 9.5VLSANNEE258 pKa = 4.15LQSLTTEE265 pKa = 5.03GIGTGTTLDD274 pKa = 4.38HH275 pKa = 6.82IMSFNSSDD283 pKa = 3.69DD284 pKa = 3.56AVEE287 pKa = 4.07MFGGRR292 pKa = 11.84VNMKK296 pKa = 9.72YY297 pKa = 9.73FVAVGAEE304 pKa = 4.14DD305 pKa = 5.43DD306 pKa = 4.99NLDD309 pKa = 3.31TDD311 pKa = 4.16TGVKK315 pKa = 10.07AQLQFVIAAQRR326 pKa = 11.84NGVGDD331 pKa = 3.79TMLEE335 pKa = 3.94ADD337 pKa = 4.56SDD339 pKa = 4.12NSVDD343 pKa = 3.71GDD345 pKa = 3.97LPRR348 pKa = 11.84QHH350 pKa = 6.08TQVANFTFLQRR361 pKa = 11.84SNVGSDD367 pKa = 3.36LAAMYY372 pKa = 10.3IRR374 pKa = 11.84GGADD378 pKa = 2.94YY379 pKa = 11.62SMANGVVSSPNYY391 pKa = 7.59TCLRR395 pKa = 11.84LSRR398 pKa = 11.84TQTIAAANAAIDD410 pKa = 3.95EE411 pKa = 4.52NGPPEE416 pKa = 4.25FEE418 pKa = 4.35SVVMDD423 pKa = 4.39CGTPTFAGNSGVTAADD439 pKa = 3.5VEE441 pKa = 5.17TVFNAGTNNDD451 pKa = 3.88DD452 pKa = 5.52DD453 pKa = 4.5FTTSLTDD460 pKa = 3.22MFVNGSNEE468 pKa = 3.97AGVTAFDD475 pKa = 4.05LTTVGIFWTDD485 pKa = 2.37TDD487 pKa = 4.29YY488 pKa = 11.45IGAVEE493 pKa = 4.75DD494 pKa = 5.98ANDD497 pKa = 3.64TWWQGWTCNSSTLAFAGSSDD517 pKa = 4.12CTSLPTSS524 pKa = 3.58

Molecular weight: 54.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6Y4E2|A0A6G6Y4E2_9SPHN L D-transpeptidase family protein OS=Sphingosinithalassobacter sp. zrk23 OX=2711215 GN=G5C33_08180 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 9.14VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3726

0

3726

1161024

38

1918

311.6

33.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.12 ± 0.06

0.785 ± 0.011

6.179 ± 0.031

6.041 ± 0.037

3.557 ± 0.024

8.912 ± 0.037

1.964 ± 0.019

4.963 ± 0.025

2.71 ± 0.032

9.714 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.021

2.41 ± 0.024

5.354 ± 0.03

3.055 ± 0.022

7.463 ± 0.043

5.265 ± 0.033

5.199 ± 0.032

7.079 ± 0.033

1.47 ± 0.017

2.245 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski