Brevundimonas abyssalis TAR-001
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2946 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U2YSP1|U2YSP1_9CAUL Probable oxidoreductase OS=Brevundimonas abyssalis TAR-001 OX=1391729 GN=MBEBAB_0711 PE=4 SV=1
MM1 pKa = 7.13 VRR3 pKa = 11.84 GPDD6 pKa = 4.37 GSWHH10 pKa = 6.67 CNDD13 pKa = 5.47 DD14 pKa = 3.8 GAGDD18 pKa = 4.56 LNPLFAATAPQSGRR32 pKa = 11.84 YY33 pKa = 8.53 DD34 pKa = 2.67 IWMGRR39 pKa = 11.84 YY40 pKa = 8.65 SQGTTNAVLNISEE53 pKa = 4.51 VIGNVSTAHH62 pKa = 6.67 GSLDD66 pKa = 4.29 DD67 pKa = 4.23 DD68 pKa = 4.94 CEE70 pKa = 4.19 YY71 pKa = 11.58 ADD73 pKa = 5.58 WDD75 pKa = 6.16 DD76 pKa = 4.08 PDD78 pKa = 3.59 CWW80 pKa = 3.77
Molecular weight: 8.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.465
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.732
Rodwell 3.528
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.151
Thurlkill 3.554
EMBOSS 3.732
Sillero 3.834
Patrickios 0.477
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|U3AY08|U3AY08_9CAUL Uncharacterized protein OS=Brevundimonas abyssalis TAR-001 OX=1391729 GN=MBEBAB_1833 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.2 NGQKK29 pKa = 9.53 IVARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.77 GRR39 pKa = 11.84 KK40 pKa = 9.04 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2946
0
2946
854767
37
1644
290.1
31.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.675 ± 0.074
0.722 ± 0.014
6.171 ± 0.035
5.893 ± 0.042
3.433 ± 0.031
9.007 ± 0.041
1.88 ± 0.022
4.413 ± 0.034
2.39 ± 0.039
9.837 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.443 ± 0.023
2.266 ± 0.028
5.537 ± 0.032
3.099 ± 0.029
7.872 ± 0.045
4.978 ± 0.033
5.313 ± 0.032
7.549 ± 0.033
1.519 ± 0.022
2.002 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here