Aspergillus cristatus
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E3BSS5|A0A1E3BSS5_9EURO Regulatory protein abaA OS=Aspergillus cristatus OX=573508 GN=SI65_01598 PE=3 SV=1
MM1 pKa = 7.4 KK2 pKa = 10.39 SIVALTSLLSLTTAAVIPNSLTRR25 pKa = 11.84 RR26 pKa = 11.84 ADD28 pKa = 3.06 LCDD31 pKa = 3.3 QYY33 pKa = 11.78 GQSTEE38 pKa = 4.07 GDD40 pKa = 3.53 FILYY44 pKa = 9.55 NNAWGKK50 pKa = 10.82 DD51 pKa = 3.54 NADD54 pKa = 4.07 SGSQCTGLDD63 pKa = 3.55 SVNGDD68 pKa = 3.86 TISWHH73 pKa = 5.94 ATWSWEE79 pKa = 4.14 GGDD82 pKa = 3.89 SSVKK86 pKa = 10.33 SFPNAAYY93 pKa = 10.41 QFDD96 pKa = 4.56 AKK98 pKa = 10.17 TLDD101 pKa = 3.92 SVSSMTTKK109 pKa = 10.17 WEE111 pKa = 3.59 WSYY114 pKa = 11.26 TGSDD118 pKa = 3.22 LVADD122 pKa = 4.16 VAYY125 pKa = 10.91 DD126 pKa = 3.52 MFLSSSADD134 pKa = 2.86 GDD136 pKa = 3.82 AEE138 pKa = 4.2 YY139 pKa = 10.76 EE140 pKa = 4.1 IMVWLAALGGAGPISSTGQSIATTTIGGTSFEE172 pKa = 4.33 LWSGKK177 pKa = 9.46 NGNMQVYY184 pKa = 9.87 SFVASSEE191 pKa = 4.05 AQNFSGDD198 pKa = 3.54 LLEE201 pKa = 4.45 FFQYY205 pKa = 10.95 LEE207 pKa = 4.2 QNQGLQSSLYY217 pKa = 9.14 LTDD220 pKa = 3.42 VQAGTEE226 pKa = 4.19 PFTGSNAKK234 pKa = 8.49 FTTSTYY240 pKa = 10.81 SVTVAA245 pKa = 4.06
Molecular weight: 26.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.948
Patrickios 0.744
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A1E3B027|A0A1E3B027_9EURO Uncharacterized protein OS=Aspergillus cristatus OX=573508 GN=SI65_10279 PE=4 SV=1
MM1 pKa = 7.53 FCRR4 pKa = 11.84 GTLPALRR11 pKa = 11.84 TSVLRR16 pKa = 11.84 AQTPLTSLNRR26 pKa = 11.84 PFSSLLTSRR35 pKa = 11.84 LSPSLPTTTTTTITPRR51 pKa = 11.84 TTLTSTFTSPLQSPLTSALNSQTRR75 pKa = 11.84 PFSASACLAGKK86 pKa = 10.27 RR87 pKa = 11.84 ITYY90 pKa = 9.51 NPSRR94 pKa = 11.84 RR95 pKa = 11.84 VQKK98 pKa = 10.2 RR99 pKa = 11.84 RR100 pKa = 11.84 HH101 pKa = 5.33 GFLARR106 pKa = 11.84 LRR108 pKa = 11.84 TRR110 pKa = 11.84 GGRR113 pKa = 11.84 KK114 pKa = 8.27 ILMRR118 pKa = 11.84 RR119 pKa = 11.84 RR120 pKa = 11.84 ARR122 pKa = 11.84 GKK124 pKa = 10.26 KK125 pKa = 9.23 ALSWW129 pKa = 3.66
Molecular weight: 14.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.399
IPC2_protein 10.95
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.223
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.945
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.114
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10115
0
10115
4808824
49
5625
475.4
52.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.324 ± 0.02
1.198 ± 0.01
5.725 ± 0.018
6.453 ± 0.029
3.662 ± 0.016
6.737 ± 0.019
2.434 ± 0.011
4.832 ± 0.017
4.828 ± 0.022
8.83 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.153 ± 0.008
3.827 ± 0.012
6.192 ± 0.029
4.288 ± 0.017
6.206 ± 0.021
8.189 ± 0.03
5.88 ± 0.017
6.028 ± 0.018
1.424 ± 0.008
2.789 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here