Microvirga guangxiensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4496 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5BFI3|A0A1G5BFI3_9RHIZ Guanylate kinase OS=Microvirga guangxiensis OX=549386 GN=gmk PE=3 SV=1
MM1 pKa = 7.23SRR3 pKa = 11.84KK4 pKa = 9.1EE5 pKa = 3.9NPFNYY10 pKa = 8.52HH11 pKa = 5.96TDD13 pKa = 3.25LSYY16 pKa = 11.48AGLMALQSEE25 pKa = 5.28MIASTLSSAVDD36 pKa = 3.41SYY38 pKa = 11.53FSVDD42 pKa = 2.8RR43 pKa = 11.84GIYY46 pKa = 9.6GGARR50 pKa = 11.84AVWGADD56 pKa = 3.07QVVGCGVDD64 pKa = 3.1WASLYY69 pKa = 10.35PADD72 pKa = 4.49EE73 pKa = 4.27VLDD76 pKa = 4.22GGGGGPGGGPVNSNLPLSCEE96 pKa = 3.97FPPEE100 pKa = 4.19GEE102 pKa = 3.8TRR104 pKa = 11.84IIIEE108 pKa = 4.25STGLPNHH115 pKa = 5.8NRR117 pKa = 11.84VIEE120 pKa = 3.88FHH122 pKa = 6.6TNGTVLIYY130 pKa = 10.21YY131 pKa = 8.3TDD133 pKa = 3.19NDD135 pKa = 3.78GNIRR139 pKa = 11.84GNQYY143 pKa = 10.88SGDD146 pKa = 3.81VQISISTTDD155 pKa = 3.3QPNLFVRR162 pKa = 11.84LEE164 pKa = 4.07EE165 pKa = 4.38YY166 pKa = 10.33PDD168 pKa = 4.18GSWVEE173 pKa = 4.21SIIDD177 pKa = 3.16IDD179 pKa = 4.35GNVLEE184 pKa = 4.63WEE186 pKa = 4.58TYY188 pKa = 8.89TVVDD192 pKa = 4.17NPDD195 pKa = 3.01GTRR198 pKa = 11.84TDD200 pKa = 2.92IYY202 pKa = 10.83YY203 pKa = 10.5DD204 pKa = 3.88SNGHH208 pKa = 5.62GSRR211 pKa = 11.84LTYY214 pKa = 10.78SSDD217 pKa = 3.87DD218 pKa = 3.46VSPWSEE224 pKa = 4.4TYY226 pKa = 10.27PDD228 pKa = 3.68DD229 pKa = 5.16AIRR232 pKa = 11.84PISSVPPPEE241 pKa = 5.23GEE243 pKa = 3.83TRR245 pKa = 11.84TVIEE249 pKa = 4.17STGVSGHH256 pKa = 5.14NRR258 pKa = 11.84VVEE261 pKa = 5.12FYY263 pKa = 11.47SNGTVLIYY271 pKa = 10.22YY272 pKa = 8.4TDD274 pKa = 3.06NDD276 pKa = 3.72GNLRR280 pKa = 11.84GNQYY284 pKa = 10.92SGDD287 pKa = 3.49VKK289 pKa = 10.32VTIKK293 pKa = 9.61EE294 pKa = 4.32TEE296 pKa = 4.0EE297 pKa = 3.53PNIYY301 pKa = 10.36YY302 pKa = 10.06RR303 pKa = 11.84VEE305 pKa = 4.1EE306 pKa = 4.35YY307 pKa = 10.3PDD309 pKa = 4.11GSWVEE314 pKa = 4.04TIIDD318 pKa = 3.16IDD320 pKa = 4.07ANVIGWEE327 pKa = 4.03SSTIIDD333 pKa = 4.31NPDD336 pKa = 3.01GTKK339 pKa = 9.98TEE341 pKa = 3.97IYY343 pKa = 8.93RR344 pKa = 11.84DD345 pKa = 3.46SMGKK349 pKa = 8.17MRR351 pKa = 11.84LTSGQSTVQYY361 pKa = 10.76GKK363 pKa = 10.92VGDD366 pKa = 3.84DD367 pKa = 3.54HH368 pKa = 8.13LLGGSGNDD376 pKa = 3.29RR377 pKa = 11.84LKK379 pKa = 11.05GASGQDD385 pKa = 3.49TLDD388 pKa = 3.47GSAGDD393 pKa = 3.85DD394 pKa = 3.51ALFGEE399 pKa = 5.17AGDD402 pKa = 4.56DD403 pKa = 3.87LLDD406 pKa = 4.35GGVGDD411 pKa = 5.28DD412 pKa = 4.43LLLGGVGNDD421 pKa = 3.41LALGGEE427 pKa = 4.55GRR429 pKa = 11.84DD430 pKa = 3.88TLDD433 pKa = 3.62GGAGNDD439 pKa = 3.73TLNGGGGFDD448 pKa = 4.39LLSGGSGDD456 pKa = 4.46DD457 pKa = 3.93VISGGSGNDD466 pKa = 3.63LLNGQDD472 pKa = 4.69GNDD475 pKa = 3.45ILQGDD480 pKa = 4.49DD481 pKa = 3.55GADD484 pKa = 3.5FLTGGAGDD492 pKa = 3.64DD493 pKa = 4.22TIRR496 pKa = 11.84GGSDD500 pKa = 3.04NDD502 pKa = 3.81SLFGNEE508 pKa = 5.06GNDD511 pKa = 3.86LLDD514 pKa = 4.27GGSGNDD520 pKa = 3.58EE521 pKa = 4.41LYY523 pKa = 11.3GLAGNDD529 pKa = 3.73MLDD532 pKa = 3.49GGAGNDD538 pKa = 4.88LMDD541 pKa = 4.95GDD543 pKa = 4.69EE544 pKa = 4.99GADD547 pKa = 3.58TLLGGAGNDD556 pKa = 3.61YY557 pKa = 10.49MRR559 pKa = 11.84GGVGADD565 pKa = 3.27VLDD568 pKa = 4.29GGEE571 pKa = 4.8GIDD574 pKa = 3.52TVDD577 pKa = 3.94YY578 pKa = 10.61SGSDD582 pKa = 2.97AGIVVNLSTGIGTGGHH598 pKa = 6.16AQDD601 pKa = 3.92DD602 pKa = 4.02VLRR605 pKa = 11.84NIEE608 pKa = 4.1NVIGSAYY615 pKa = 10.33NDD617 pKa = 4.06LLVGHH622 pKa = 7.58AGHH625 pKa = 7.28DD626 pKa = 3.9VLWGNDD632 pKa = 3.57GNDD635 pKa = 4.0TIHH638 pKa = 7.09GGLGNDD644 pKa = 3.94TLHH647 pKa = 7.1GEE649 pKa = 4.3AGDD652 pKa = 3.84DD653 pKa = 3.88HH654 pKa = 6.53MSGGAGHH661 pKa = 7.06DD662 pKa = 3.8TLHH665 pKa = 6.83GGDD668 pKa = 4.69GQDD671 pKa = 3.15QLYY674 pKa = 10.98GEE676 pKa = 5.49DD677 pKa = 4.14GNDD680 pKa = 3.79ALRR683 pKa = 11.84GDD685 pKa = 4.39AGNDD689 pKa = 3.26SLYY692 pKa = 11.21GGAGSDD698 pKa = 3.64EE699 pKa = 5.25LIGGTGDD706 pKa = 3.3DD707 pKa = 4.37HH708 pKa = 7.26LAGGLDD714 pKa = 3.6QDD716 pKa = 3.78WLYY719 pKa = 11.6GGDD722 pKa = 3.9GQDD725 pKa = 2.69WLYY728 pKa = 11.64GEE730 pKa = 5.35DD731 pKa = 4.46GNDD734 pKa = 3.13ILHH737 pKa = 6.07GQNGNDD743 pKa = 3.49YY744 pKa = 10.88LYY746 pKa = 11.04GGNGNEE752 pKa = 4.07LDD754 pKa = 3.64AGGSVFLEE762 pKa = 4.97GIKK765 pKa = 10.47AEE767 pKa = 3.97QLKK770 pKa = 10.99GGIVDD775 pKa = 5.64GAWQWWMM782 pKa = 3.74

Molecular weight: 81.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5JKX3|A0A1G5JKX3_9RHIZ Predicted Zn-dependent peptidase OS=Microvirga guangxiensis OX=549386 GN=SAMN02927923_02713 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.23KK3 pKa = 9.93ARR5 pKa = 11.84PVSRR9 pKa = 11.84VARR12 pKa = 11.84AVPGAARR19 pKa = 11.84KK20 pKa = 9.13VVSGPSVAVAVPAEE34 pKa = 3.74ARR36 pKa = 11.84LRR38 pKa = 11.84HH39 pKa = 5.87PVQPPARR46 pKa = 11.84ARR48 pKa = 11.84SGSHH52 pKa = 5.46LRR54 pKa = 11.84RR55 pKa = 11.84HH56 pKa = 5.64RR57 pKa = 11.84VLLKK61 pKa = 10.55SNVRR65 pKa = 11.84NRR67 pKa = 11.84GEE69 pKa = 4.01NSASRR74 pKa = 11.84QNALPRR80 pKa = 11.84QLLPSRR86 pKa = 11.84GQPLRR91 pKa = 11.84NEE93 pKa = 3.57LRR95 pKa = 11.84TRR97 pKa = 11.84HH98 pKa = 5.74LRR100 pKa = 11.84GLSAQTLRR108 pKa = 11.84SVSVPPAPMHH118 pKa = 6.62RR119 pKa = 11.84SANSVPQSASALRR132 pKa = 11.84LPMPRR137 pKa = 11.84RR138 pKa = 11.84GLRR141 pKa = 11.84RR142 pKa = 11.84PLLLNRR148 pKa = 11.84PRR150 pKa = 11.84RR151 pKa = 11.84LPRR154 pKa = 11.84QSLEE158 pKa = 3.62RR159 pKa = 11.84HH160 pKa = 5.73RR161 pKa = 11.84PRR163 pKa = 11.84LAPAMRR169 pKa = 11.84NVPIKK174 pKa = 10.52VSRR177 pKa = 11.84GPLRR181 pKa = 11.84HH182 pKa = 6.41LPPPSRR188 pKa = 11.84SGAHH192 pKa = 5.3RR193 pKa = 11.84QQ194 pKa = 3.23

Molecular weight: 21.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4496

0

4496

1369810

39

3014

304.7

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.654 ± 0.046

0.781 ± 0.011

5.408 ± 0.032

5.978 ± 0.036

3.724 ± 0.023

8.406 ± 0.043

2.018 ± 0.019

5.474 ± 0.027

3.552 ± 0.029

10.219 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.015

2.732 ± 0.026

5.162 ± 0.033

3.269 ± 0.019

7.03 ± 0.039

5.788 ± 0.027

5.324 ± 0.03

7.459 ± 0.027

1.297 ± 0.016

2.241 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski