Chino del tomate Amazonas virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9KZK8|E9KZK8_9GEMI Replication-associated protein OS=Chino del tomate Amazonas virus OX=858516 GN=AC1 PE=3 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84SSSPSAPPSIKK13 pKa = 9.69RR14 pKa = 11.84AHH16 pKa = 5.99RR17 pKa = 11.84QAKK20 pKa = 8.85KK21 pKa = 9.0RR22 pKa = 11.84AIRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84IDD30 pKa = 3.78LEE32 pKa = 4.52CGCSIYY38 pKa = 11.2LHH40 pKa = 6.75IGCTGHH46 pKa = 6.92GFTHH50 pKa = 7.49RR51 pKa = 11.84GTHH54 pKa = 5.78HH55 pKa = 6.16CTSGRR60 pKa = 11.84EE61 pKa = 3.81WRR63 pKa = 11.84VYY65 pKa = 10.84LGDD68 pKa = 3.55NKK70 pKa = 10.88SPIFQDD76 pKa = 2.83IQRR79 pKa = 11.84RR80 pKa = 11.84DD81 pKa = 3.48THH83 pKa = 5.04VHH85 pKa = 5.47EE86 pKa = 4.69LQGVSHH92 pKa = 7.06TNTVQSQPQEE102 pKa = 4.3GVEE105 pKa = 4.43STQSHH110 pKa = 7.1PEE112 pKa = 3.94FPSLDD117 pKa = 4.61DD118 pKa = 4.15ISDD121 pKa = 4.01SFWDD125 pKa = 5.14DD126 pKa = 2.62IFKK129 pKa = 11.03

Molecular weight: 14.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9KZK8|E9KZK8_9GEMI Replication-associated protein OS=Chino del tomate Amazonas virus OX=858516 GN=AC1 PE=3 SV=1
MM1 pKa = 7.18SQFSEE6 pKa = 4.14SSNWGDD12 pKa = 3.88KK13 pKa = 10.67PILPKK18 pKa = 10.3CHH20 pKa = 6.59HH21 pKa = 6.82PSVSKK26 pKa = 10.74SLPKK30 pKa = 10.12TISLLILIALSPKK43 pKa = 9.49TMHH46 pKa = 6.61FPNSSTFKK54 pKa = 10.35PQLTNYY60 pKa = 10.16SFVLQEE66 pKa = 3.78SFTRR70 pKa = 11.84MGSLISMCLSNSKK83 pKa = 10.92GNTNAPTTDD92 pKa = 2.73SSTWYY97 pKa = 8.26PQPDD101 pKa = 3.2QHH103 pKa = 8.64ISIRR107 pKa = 11.84TFRR110 pKa = 11.84EE111 pKa = 3.78LKK113 pKa = 10.48KK114 pKa = 10.21QAQTSKK120 pKa = 10.42PIWRR124 pKa = 11.84KK125 pKa = 7.83TEE127 pKa = 3.48TSLIMEE133 pKa = 5.58FSRR136 pKa = 11.84SMDD139 pKa = 3.52DD140 pKa = 3.58QLEE143 pKa = 4.27EE144 pKa = 4.17VANLPTTLMPRR155 pKa = 11.84LSMQAMQPRR164 pKa = 11.84PSIYY168 pKa = 9.77SEE170 pKa = 4.02RR171 pKa = 11.84NNPEE175 pKa = 3.5TFLQIIIISKK185 pKa = 9.53QMQKK189 pKa = 10.79GFLLRR194 pKa = 11.84LRR196 pKa = 11.84NHH198 pKa = 6.42GFLRR202 pKa = 11.84FPSPHH207 pKa = 6.65SLTFQKK213 pKa = 10.28RR214 pKa = 11.84CKK216 pKa = 9.76SGQIIISGEE225 pKa = 3.83VPLRR229 pKa = 11.84GRR231 pKa = 11.84RR232 pKa = 11.84DD233 pKa = 3.43RR234 pKa = 11.84LVSSSRR240 pKa = 11.84VNSRR244 pKa = 11.84TGKK247 pKa = 8.38TMWARR252 pKa = 11.84ALGPHH257 pKa = 6.86TYY259 pKa = 10.87LSGHH263 pKa = 7.06LDD265 pKa = 3.61FNGTVYY271 pKa = 10.85SNQVEE276 pKa = 4.58YY277 pKa = 10.36TVIAAVAPHH286 pKa = 5.5YY287 pKa = 10.87LKK289 pKa = 10.79LKK291 pKa = 9.12HH292 pKa = 5.78WKK294 pKa = 9.68EE295 pKa = 4.26LIGSQKK301 pKa = 10.68DD302 pKa = 3.23WQSNCKK308 pKa = 9.38YY309 pKa = 9.74GKK311 pKa = 9.18PVQIQGGIPSIVLCNPGSGFQLSRR335 pKa = 11.84FPQQRR340 pKa = 11.84GKK342 pKa = 11.01RR343 pKa = 11.84ITQEE347 pKa = 4.33LDD349 pKa = 3.19PQEE352 pKa = 4.86CALHH356 pKa = 6.66HH357 pKa = 6.4PQLPPQQ363 pKa = 3.65

Molecular weight: 41.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

883

125

363

220.8

25.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.077 ± 0.689

1.812 ± 0.388

3.398 ± 0.688

4.19 ± 0.342

4.077 ± 0.047

5.776 ± 0.81

4.19 ± 0.744

6.455 ± 0.388

5.436 ± 0.559

7.701 ± 1.14

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.945 ± 0.514

5.21 ± 1.155

5.663 ± 1.353

5.323 ± 1.264

7.135 ± 0.704

9.626 ± 1.484

6.455 ± 0.666

5.436 ± 1.332

1.586 ± 0.038

3.511 ± 0.952
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski