Plasticicumulans acidivorans
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3852 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A317MRI1|A0A317MRI1_9GAMM 30S ribosomal protein S1 OS=Plasticicumulans acidivorans OX=886464 GN=C7443_11036 PE=3 SV=1
MM1 pKa = 7.09 TKK3 pKa = 10.52 KK4 pKa = 10.15 NALSALVGLALAAPLGAQAALLFDD28 pKa = 4.98 PDD30 pKa = 3.97 GAGAAYY36 pKa = 7.61 GTYY39 pKa = 10.3 AIDD42 pKa = 4.17 LLDD45 pKa = 3.91 WSPTSIIAVGGNQAIQNYY63 pKa = 9.11 IYY65 pKa = 10.3 NATYY69 pKa = 8.06 GTSLDD74 pKa = 3.75 TTFTVYY80 pKa = 10.03 TMAKK84 pKa = 10.17 LSTGQLNSNNQFTMGGSSEE103 pKa = 3.97 ITVLLGYY110 pKa = 7.72 TATVTFADD118 pKa = 3.71 ATTGISTFAQVAGSNFVEE136 pKa = 5.06 MYY138 pKa = 11.05 YY139 pKa = 10.96 DD140 pKa = 3.99 DD141 pKa = 5.17 TPDD144 pKa = 4.25 ADD146 pKa = 4.22 PLTGTGFSDD155 pKa = 3.65 GQVIFSSTVTAQNGFFSADD174 pKa = 3.19 QSGATTTLDD183 pKa = 3.16 QSANGDD189 pKa = 3.48 DD190 pKa = 3.4 WGGQQTVTGVGISGNVASVLPATVIDD216 pKa = 3.6 TTFFKK221 pKa = 10.69 SVPLLTFITTNISLNTPFTSVDD243 pKa = 3.37 PSQGYY248 pKa = 10.19 YY249 pKa = 9.1 STTGLAAPDD258 pKa = 3.39 IAPNIGTVNGSLTSGGVDD276 pKa = 4.65 FIFSSDD282 pKa = 3.28 FNTSVNAVPEE292 pKa = 4.25 PASLALLGIGLGAIGTLANRR312 pKa = 11.84 RR313 pKa = 11.84 RR314 pKa = 11.84 KK315 pKa = 7.91 TKK317 pKa = 10.56
Molecular weight: 32.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.503
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.77
Rodwell 3.567
Grimsley 3.414
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.215
Thurlkill 3.579
EMBOSS 3.77
Sillero 3.872
Patrickios 0.998
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|A0A317MZ59|A0A317MZ59_9GAMM Single-strand selective monofunctional uracil DNA glycosylase OS=Plasticicumulans acidivorans OX=886464 GN=C7443_10165 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 8.98 RR4 pKa = 11.84 TFQPSVIHH12 pKa = 6.94 RR13 pKa = 11.84 KK14 pKa = 7.6 RR15 pKa = 11.84 THH17 pKa = 5.78 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.41 NGRR29 pKa = 11.84 LVINARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.618
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.34
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3852
0
3852
1284836
28
2843
333.6
36.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.354 ± 0.072
1.141 ± 0.015
5.476 ± 0.029
5.661 ± 0.038
3.401 ± 0.028
8.33 ± 0.044
2.335 ± 0.02
4.346 ± 0.031
2.271 ± 0.03
11.988 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.988 ± 0.017
2.256 ± 0.022
5.207 ± 0.028
3.671 ± 0.03
7.767 ± 0.045
5.138 ± 0.038
4.728 ± 0.034
7.192 ± 0.037
1.459 ± 0.017
2.291 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here