Mycobacterium phage Wheeler

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5Z8T9|S5Z8T9_9CAUD Minor tail protein OS=Mycobacterium phage Wheeler OX=1383054 GN=31 PE=4 SV=1
MM1 pKa = 7.93KK2 pKa = 9.35YY3 pKa = 7.78TLRR6 pKa = 11.84VFGIPVLSFEE16 pKa = 4.47SAGTGAEE23 pKa = 3.56EE24 pKa = 5.43GYY26 pKa = 10.65INLTGGSFEE35 pKa = 4.65LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5Y7M1|S5Y7M1_9CAUD Uncharacterized protein OS=Mycobacterium phage Wheeler OX=1383054 GN=48 PE=4 SV=1
MM1 pKa = 7.57SKK3 pKa = 10.36HH4 pKa = 5.97EE5 pKa = 4.13YY6 pKa = 10.38VGGFTADD13 pKa = 3.56PEE15 pKa = 4.5QFAHH19 pKa = 6.75LWVQGHH25 pKa = 5.95LARR28 pKa = 11.84RR29 pKa = 11.84LGLTHH34 pKa = 6.01VRR36 pKa = 11.84EE37 pKa = 4.68SVSGRR42 pKa = 11.84HH43 pKa = 5.61RR44 pKa = 11.84LPDD47 pKa = 3.18VRR49 pKa = 11.84FSQEE53 pKa = 3.84LPGGTVYY60 pKa = 10.53WSVNRR65 pKa = 11.84KK66 pKa = 9.3GFFRR70 pKa = 11.84RR71 pKa = 11.84DD72 pKa = 3.17DD73 pKa = 3.85SLPSGWVQRR82 pKa = 11.84IYY84 pKa = 10.86PRR86 pKa = 11.84VATSFRR92 pKa = 11.84TAEE95 pKa = 3.85

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

16979

25

825

176.9

19.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.789 ± 0.374

0.813 ± 0.122

6.673 ± 0.218

6.449 ± 0.23

3.122 ± 0.154

8.74 ± 0.55

2.097 ± 0.155

4.812 ± 0.235

4.288 ± 0.242

8.363 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.038 ± 0.116

3.269 ± 0.134

5.519 ± 0.2

3.163 ± 0.15

6.39 ± 0.369

6.002 ± 0.247

6.172 ± 0.217

7.285 ± 0.221

2.008 ± 0.121

3.01 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski