Amino acid dipepetide frequency for Mycobacterium phage Wheeler

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.899AlaAla: 12.899 ± 1.361
0.766AlaCys: 0.766 ± 0.186
6.95AlaAsp: 6.95 ± 0.569
5.949AlaGlu: 5.949 ± 0.607
2.945AlaPhe: 2.945 ± 0.47
7.893AlaGly: 7.893 ± 0.677
1.708AlaHis: 1.708 ± 0.376
3.887AlaIle: 3.887 ± 0.501
4.182AlaLys: 4.182 ± 0.569
8.953AlaLeu: 8.953 ± 0.854
2.592AlaMet: 2.592 ± 0.434
2.65AlaAsn: 2.65 ± 0.353
4.83AlaPro: 4.83 ± 0.685
2.592AlaGln: 2.592 ± 0.441
5.772AlaArg: 5.772 ± 0.557
5.183AlaSer: 5.183 ± 0.601
5.242AlaThr: 5.242 ± 0.559
8.069AlaVal: 8.069 ± 0.74
1.708AlaTrp: 1.708 ± 0.313
3.063AlaTyr: 3.063 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.766CysAla: 0.766 ± 0.253
0.059CysCys: 0.059 ± 0.067
0.589CysAsp: 0.589 ± 0.244
0.766CysGlu: 0.766 ± 0.244
0.118CysPhe: 0.118 ± 0.08
0.942CysGly: 0.942 ± 0.3
0.294CysHis: 0.294 ± 0.152
0.294CysIle: 0.294 ± 0.154
0.353CysLys: 0.353 ± 0.169
0.353CysLeu: 0.353 ± 0.175
0.118CysMet: 0.118 ± 0.087
0.412CysAsn: 0.412 ± 0.185
0.294CysPro: 0.294 ± 0.126
0.412CysGln: 0.412 ± 0.16
0.589CysArg: 0.589 ± 0.171
0.412CysSer: 0.412 ± 0.145
0.412CysThr: 0.412 ± 0.172
0.353CysVal: 0.353 ± 0.188
0.353CysTrp: 0.353 ± 0.123
0.236CysTyr: 0.236 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
6.361AspAla: 6.361 ± 0.54
0.471AspCys: 0.471 ± 0.172
4.3AspAsp: 4.3 ± 0.497
3.711AspGlu: 3.711 ± 0.491
2.886AspPhe: 2.886 ± 0.371
5.89AspGly: 5.89 ± 0.646
1.472AspHis: 1.472 ± 0.295
2.592AspIle: 2.592 ± 0.439
2.945AspLys: 2.945 ± 0.454
6.773AspLeu: 6.773 ± 0.704
1.119AspMet: 1.119 ± 0.214
2.003AspAsn: 2.003 ± 0.33
5.006AspPro: 5.006 ± 0.577
1.472AspGln: 1.472 ± 0.308
3.887AspArg: 3.887 ± 0.402
3.593AspSer: 3.593 ± 0.442
3.593AspThr: 3.593 ± 0.374
5.006AspVal: 5.006 ± 0.585
1.708AspTrp: 1.708 ± 0.275
1.944AspTyr: 1.944 ± 0.296
0.0AspXaa: 0.0 ± 0.0
Glu
6.479GluAla: 6.479 ± 0.569
0.353GluCys: 0.353 ± 0.149
4.653GluAsp: 4.653 ± 0.437
4.535GluGlu: 4.535 ± 0.604
1.885GluPhe: 1.885 ± 0.275
3.77GluGly: 3.77 ± 0.412
1.237GluHis: 1.237 ± 0.292
3.239GluIle: 3.239 ± 0.442
2.886GluLys: 2.886 ± 0.445
6.891GluLeu: 6.891 ± 0.611
1.531GluMet: 1.531 ± 0.266
1.531GluAsn: 1.531 ± 0.344
2.533GluPro: 2.533 ± 0.347
2.709GluGln: 2.709 ± 0.417
3.711GluArg: 3.711 ± 0.474
3.239GluSer: 3.239 ± 0.403
3.475GluThr: 3.475 ± 0.487
5.772GluVal: 5.772 ± 0.64
1.531GluTrp: 1.531 ± 0.288
2.533GluTyr: 2.533 ± 0.432
0.0GluXaa: 0.0 ± 0.0
Phe
2.474PheAla: 2.474 ± 0.383
0.294PheCys: 0.294 ± 0.156
2.768PheAsp: 2.768 ± 0.355
2.061PheGlu: 2.061 ± 0.304
0.589PhePhe: 0.589 ± 0.179
3.475PheGly: 3.475 ± 0.456
0.883PheHis: 0.883 ± 0.263
1.414PheIle: 1.414 ± 0.245
1.178PheLys: 1.178 ± 0.273
2.474PheLeu: 2.474 ± 0.387
0.53PheMet: 0.53 ± 0.175
1.237PheAsn: 1.237 ± 0.268
1.708PhePro: 1.708 ± 0.336
0.825PheGln: 0.825 ± 0.217
2.003PheArg: 2.003 ± 0.387
1.944PheSer: 1.944 ± 0.293
1.944PheThr: 1.944 ± 0.319
1.944PheVal: 1.944 ± 0.372
0.589PheTrp: 0.589 ± 0.162
0.883PheTyr: 0.883 ± 0.199
0.0PheXaa: 0.0 ± 0.0
Gly
7.421GlyAla: 7.421 ± 0.86
0.589GlyCys: 0.589 ± 0.174
5.713GlyAsp: 5.713 ± 0.42
4.594GlyGlu: 4.594 ± 0.519
2.533GlyPhe: 2.533 ± 0.441
10.19GlyGly: 10.19 ± 2.139
2.238GlyHis: 2.238 ± 0.368
4.535GlyIle: 4.535 ± 0.646
3.475GlyLys: 3.475 ± 0.542
7.068GlyLeu: 7.068 ± 0.703
2.12GlyMet: 2.12 ± 0.337
3.298GlyAsn: 3.298 ± 0.428
3.887GlyPro: 3.887 ± 0.494
2.415GlyGln: 2.415 ± 0.367
4.83GlyArg: 4.83 ± 0.597
6.538GlySer: 6.538 ± 0.948
5.301GlyThr: 5.301 ± 0.723
5.124GlyVal: 5.124 ± 0.564
2.592GlyTrp: 2.592 ± 0.351
2.945GlyTyr: 2.945 ± 0.39
0.0GlyXaa: 0.0 ± 0.0
His
2.061HisAla: 2.061 ± 0.369
0.236HisCys: 0.236 ± 0.161
1.178HisAsp: 1.178 ± 0.239
1.708HisGlu: 1.708 ± 0.309
0.707HisPhe: 0.707 ± 0.182
1.767HisGly: 1.767 ± 0.383
0.648HisHis: 0.648 ± 0.2
0.883HisIle: 0.883 ± 0.257
1.178HisLys: 1.178 ± 0.283
1.531HisLeu: 1.531 ± 0.311
0.177HisMet: 0.177 ± 0.106
0.236HisAsn: 0.236 ± 0.114
1.296HisPro: 1.296 ± 0.262
0.707HisGln: 0.707 ± 0.182
1.649HisArg: 1.649 ± 0.329
0.766HisSer: 0.766 ± 0.199
1.296HisThr: 1.296 ± 0.28
1.826HisVal: 1.826 ± 0.328
0.53HisTrp: 0.53 ± 0.154
0.589HisTyr: 0.589 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
5.537IleAla: 5.537 ± 0.575
0.471IleCys: 0.471 ± 0.168
3.416IleAsp: 3.416 ± 0.381
3.652IleGlu: 3.652 ± 0.427
0.825IlePhe: 0.825 ± 0.231
4.005IleGly: 4.005 ± 0.446
0.883IleHis: 0.883 ± 0.199
1.944IleIle: 1.944 ± 0.362
1.708IleLys: 1.708 ± 0.279
3.122IleLeu: 3.122 ± 0.42
0.707IleMet: 0.707 ± 0.174
1.708IleAsn: 1.708 ± 0.29
3.181IlePro: 3.181 ± 0.359
1.472IleGln: 1.472 ± 0.272
3.416IleArg: 3.416 ± 0.457
3.181IleSer: 3.181 ± 0.481
3.416IleThr: 3.416 ± 0.502
3.004IleVal: 3.004 ± 0.484
0.707IleTrp: 0.707 ± 0.204
1.767IleTyr: 1.767 ± 0.27
0.0IleXaa: 0.0 ± 0.0
Lys
4.005LysAla: 4.005 ± 0.466
0.589LysCys: 0.589 ± 0.196
2.886LysAsp: 2.886 ± 0.451
2.003LysGlu: 2.003 ± 0.321
1.355LysPhe: 1.355 ± 0.252
2.886LysGly: 2.886 ± 0.382
1.296LysHis: 1.296 ± 0.365
2.238LysIle: 2.238 ± 0.405
2.474LysLys: 2.474 ± 0.546
3.004LysLeu: 3.004 ± 0.414
1.001LysMet: 1.001 ± 0.211
1.414LysAsn: 1.414 ± 0.224
2.474LysPro: 2.474 ± 0.363
1.826LysGln: 1.826 ± 0.412
3.181LysArg: 3.181 ± 0.549
2.356LysSer: 2.356 ± 0.412
2.415LysThr: 2.415 ± 0.344
3.416LysVal: 3.416 ± 0.395
0.648LysTrp: 0.648 ± 0.199
1.414LysTyr: 1.414 ± 0.293
0.0LysXaa: 0.0 ± 0.0
Leu
8.717LeuAla: 8.717 ± 0.745
0.353LeuCys: 0.353 ± 0.133
6.597LeuAsp: 6.597 ± 0.604
5.006LeuGlu: 5.006 ± 0.467
2.415LeuPhe: 2.415 ± 0.357
7.304LeuGly: 7.304 ± 0.616
1.531LeuHis: 1.531 ± 0.302
4.594LeuIle: 4.594 ± 0.471
3.887LeuLys: 3.887 ± 0.473
5.831LeuLeu: 5.831 ± 0.486
1.708LeuMet: 1.708 ± 0.261
3.063LeuAsn: 3.063 ± 0.388
4.948LeuPro: 4.948 ± 0.61
2.827LeuGln: 2.827 ± 0.486
6.008LeuArg: 6.008 ± 0.483
5.065LeuSer: 5.065 ± 0.47
5.89LeuThr: 5.89 ± 0.512
4.417LeuVal: 4.417 ± 0.512
1.119LeuTrp: 1.119 ± 0.305
2.356LeuTyr: 2.356 ± 0.407
0.0LeuXaa: 0.0 ± 0.0
Met
2.179MetAla: 2.179 ± 0.255
0.0MetCys: 0.0 ± 0.0
1.06MetAsp: 1.06 ± 0.238
1.472MetGlu: 1.472 ± 0.283
0.648MetPhe: 0.648 ± 0.191
1.355MetGly: 1.355 ± 0.236
0.353MetHis: 0.353 ± 0.143
0.883MetIle: 0.883 ± 0.267
1.119MetLys: 1.119 ± 0.24
1.237MetLeu: 1.237 ± 0.244
0.177MetMet: 0.177 ± 0.108
1.001MetAsn: 1.001 ± 0.205
1.355MetPro: 1.355 ± 0.238
0.589MetGln: 0.589 ± 0.163
1.001MetArg: 1.001 ± 0.245
2.179MetSer: 2.179 ± 0.372
1.885MetThr: 1.885 ± 0.294
1.355MetVal: 1.355 ± 0.294
0.177MetTrp: 0.177 ± 0.094
0.353MetTyr: 0.353 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.063AsnAla: 3.063 ± 0.456
0.118AsnCys: 0.118 ± 0.092
1.944AsnAsp: 1.944 ± 0.351
1.885AsnGlu: 1.885 ± 0.327
0.942AsnPhe: 0.942 ± 0.251
3.475AsnGly: 3.475 ± 0.424
0.766AsnHis: 0.766 ± 0.19
1.767AsnIle: 1.767 ± 0.298
0.766AsnLys: 0.766 ± 0.243
2.533AsnLeu: 2.533 ± 0.348
0.471AsnMet: 0.471 ± 0.17
0.942AsnAsn: 0.942 ± 0.202
3.004AsnPro: 3.004 ± 0.355
1.119AsnGln: 1.119 ± 0.192
1.708AsnArg: 1.708 ± 0.384
1.649AsnSer: 1.649 ± 0.357
2.003AsnThr: 2.003 ± 0.323
2.533AsnVal: 2.533 ± 0.424
0.766AsnTrp: 0.766 ± 0.206
1.237AsnTyr: 1.237 ± 0.243
0.0AsnXaa: 0.0 ± 0.0
Pro
4.889ProAla: 4.889 ± 0.56
0.648ProCys: 0.648 ± 0.254
4.182ProAsp: 4.182 ± 0.403
4.712ProGlu: 4.712 ± 0.555
2.297ProPhe: 2.297 ± 0.431
4.948ProGly: 4.948 ± 0.558
0.825ProHis: 0.825 ± 0.201
2.179ProIle: 2.179 ± 0.347
2.415ProLys: 2.415 ± 0.374
4.653ProLeu: 4.653 ± 0.603
0.942ProMet: 0.942 ± 0.259
1.649ProAsn: 1.649 ± 0.306
3.122ProPro: 3.122 ± 0.535
1.531ProGln: 1.531 ± 0.31
2.592ProArg: 2.592 ± 0.413
3.828ProSer: 3.828 ± 0.503
3.475ProThr: 3.475 ± 0.44
3.887ProVal: 3.887 ± 0.472
0.766ProTrp: 0.766 ± 0.268
1.649ProTyr: 1.649 ± 0.355
0.0ProXaa: 0.0 ± 0.0
Gln
2.945GlnAla: 2.945 ± 0.443
0.236GlnCys: 0.236 ± 0.128
1.649GlnAsp: 1.649 ± 0.366
1.531GlnGlu: 1.531 ± 0.252
1.06GlnPhe: 1.06 ± 0.217
2.474GlnGly: 2.474 ± 0.337
0.471GlnHis: 0.471 ± 0.147
2.65GlnIle: 2.65 ± 0.483
1.001GlnLys: 1.001 ± 0.262
3.593GlnLeu: 3.593 ± 0.496
0.825GlnMet: 0.825 ± 0.203
0.412GlnAsn: 0.412 ± 0.122
1.59GlnPro: 1.59 ± 0.317
1.59GlnGln: 1.59 ± 0.346
2.12GlnArg: 2.12 ± 0.323
2.003GlnSer: 2.003 ± 0.339
1.885GlnThr: 1.885 ± 0.338
2.238GlnVal: 2.238 ± 0.331
0.707GlnTrp: 0.707 ± 0.164
0.648GlnTyr: 0.648 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
5.36ArgAla: 5.36 ± 0.61
1.001ArgCys: 1.001 ± 0.257
3.122ArgAsp: 3.122 ± 0.398
4.948ArgGlu: 4.948 ± 0.62
1.767ArgPhe: 1.767 ± 0.33
5.478ArgGly: 5.478 ± 0.675
1.119ArgHis: 1.119 ± 0.28
3.004ArgIle: 3.004 ± 0.426
3.652ArgLys: 3.652 ± 0.613
5.772ArgLeu: 5.772 ± 0.685
1.885ArgMet: 1.885 ± 0.34
2.827ArgAsn: 2.827 ± 0.538
2.356ArgPro: 2.356 ± 0.359
1.944ArgGln: 1.944 ± 0.314
5.36ArgArg: 5.36 ± 0.662
3.063ArgSer: 3.063 ± 0.455
2.886ArgThr: 2.886 ± 0.459
5.242ArgVal: 5.242 ± 0.58
1.296ArgTrp: 1.296 ± 0.323
1.826ArgTyr: 1.826 ± 0.242
0.0ArgXaa: 0.0 ± 0.0
Ser
6.42SerAla: 6.42 ± 0.743
0.648SerCys: 0.648 ± 0.226
3.357SerAsp: 3.357 ± 0.458
3.711SerGlu: 3.711 ± 0.463
2.061SerPhe: 2.061 ± 0.441
6.42SerGly: 6.42 ± 0.954
1.531SerHis: 1.531 ± 0.319
3.004SerIle: 3.004 ± 0.386
2.061SerLys: 2.061 ± 0.276
4.594SerLeu: 4.594 ± 0.579
1.472SerMet: 1.472 ± 0.273
2.415SerAsn: 2.415 ± 0.398
3.416SerPro: 3.416 ± 0.488
2.061SerGln: 2.061 ± 0.298
2.827SerArg: 2.827 ± 0.375
3.593SerSer: 3.593 ± 0.539
3.298SerThr: 3.298 ± 0.471
4.3SerVal: 4.3 ± 0.457
1.355SerTrp: 1.355 ± 0.305
1.472SerTyr: 1.472 ± 0.265
0.0SerXaa: 0.0 ± 0.0
Thr
5.654ThrAla: 5.654 ± 0.636
0.294ThrCys: 0.294 ± 0.161
4.241ThrAsp: 4.241 ± 0.43
4.241ThrGlu: 4.241 ± 0.528
2.003ThrPhe: 2.003 ± 0.294
6.302ThrGly: 6.302 ± 0.56
1.237ThrHis: 1.237 ± 0.317
2.533ThrIle: 2.533 ± 0.481
2.709ThrLys: 2.709 ± 0.319
5.419ThrLeu: 5.419 ± 0.598
0.825ThrMet: 0.825 ± 0.215
1.767ThrAsn: 1.767 ± 0.316
3.711ThrPro: 3.711 ± 0.475
1.414ThrGln: 1.414 ± 0.266
3.77ThrArg: 3.77 ± 0.498
3.711ThrSer: 3.711 ± 0.642
3.828ThrThr: 3.828 ± 0.56
5.183ThrVal: 5.183 ± 0.564
1.119ThrTrp: 1.119 ± 0.251
1.767ThrTyr: 1.767 ± 0.327
0.0ThrXaa: 0.0 ± 0.0
Val
6.479ValAla: 6.479 ± 0.672
0.471ValCys: 0.471 ± 0.149
5.183ValAsp: 5.183 ± 0.525
4.771ValGlu: 4.771 ± 0.483
2.533ValPhe: 2.533 ± 0.356
4.653ValGly: 4.653 ± 0.577
1.355ValHis: 1.355 ± 0.257
3.77ValIle: 3.77 ± 0.499
3.122ValLys: 3.122 ± 0.433
5.36ValLeu: 5.36 ± 0.553
1.119ValMet: 1.119 ± 0.271
2.592ValAsn: 2.592 ± 0.346
4.417ValPro: 4.417 ± 0.492
2.238ValGln: 2.238 ± 0.339
5.772ValArg: 5.772 ± 0.746
4.889ValSer: 4.889 ± 0.559
5.301ValThr: 5.301 ± 0.505
5.124ValVal: 5.124 ± 0.625
1.355ValTrp: 1.355 ± 0.315
2.356ValTyr: 2.356 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
1.767TrpAla: 1.767 ± 0.39
0.236TrpCys: 0.236 ± 0.117
1.414TrpAsp: 1.414 ± 0.273
0.942TrpGlu: 0.942 ± 0.197
0.825TrpPhe: 0.825 ± 0.189
1.59TrpGly: 1.59 ± 0.294
0.471TrpHis: 0.471 ± 0.177
1.237TrpIle: 1.237 ± 0.266
0.353TrpLys: 0.353 ± 0.164
1.826TrpLeu: 1.826 ± 0.286
0.412TrpMet: 0.412 ± 0.176
0.353TrpAsn: 0.353 ± 0.114
0.648TrpPro: 0.648 ± 0.221
0.883TrpGln: 0.883 ± 0.18
1.119TrpArg: 1.119 ± 0.277
1.178TrpSer: 1.178 ± 0.234
1.885TrpThr: 1.885 ± 0.356
2.003TrpVal: 2.003 ± 0.361
0.648TrpTrp: 0.648 ± 0.246
0.294TrpTyr: 0.294 ± 0.117
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.415TyrAla: 2.415 ± 0.387
0.294TyrCys: 0.294 ± 0.121
1.531TyrAsp: 1.531 ± 0.341
2.297TyrGlu: 2.297 ± 0.335
0.825TyrPhe: 0.825 ± 0.217
2.592TyrGly: 2.592 ± 0.384
0.648TyrHis: 0.648 ± 0.224
1.472TyrIle: 1.472 ± 0.283
1.414TyrLys: 1.414 ± 0.314
2.65TyrLeu: 2.65 ± 0.405
0.589TyrMet: 0.589 ± 0.173
1.178TyrAsn: 1.178 ± 0.273
1.414TyrPro: 1.414 ± 0.272
1.001TyrGln: 1.001 ± 0.254
2.592TyrArg: 2.592 ± 0.357
1.649TyrSer: 1.649 ± 0.293
2.297TyrThr: 2.297 ± 0.423
2.061TyrVal: 2.061 ± 0.385
0.412TyrTrp: 0.412 ± 0.148
0.766TyrTyr: 0.766 ± 0.253
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (16979 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski