Sphingomonas sp. Root720

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5044 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8R3A1|A0A0Q8R3A1_9SPHN Peptidase M28 OS=Sphingomonas sp. Root720 OX=1736595 GN=ASE22_14830 PE=4 SV=1
MM1 pKa = 8.78VMVTPVGDD9 pKa = 3.6APVYY13 pKa = 9.71MGSLFDD19 pKa = 4.43FVAVKK24 pKa = 9.59PRR26 pKa = 11.84PEE28 pKa = 4.94AIHH31 pKa = 5.91VQTTTSWIFDD41 pKa = 3.82DD42 pKa = 3.66QVGRR46 pKa = 11.84SSYY49 pKa = 10.97SGIGFTYY56 pKa = 10.14DD57 pKa = 3.09AQGLLVSGTITGLHH71 pKa = 6.06WSAPVYY77 pKa = 10.6GAFDD81 pKa = 3.49VGGLAISAAAFRR93 pKa = 11.84QSWAGGDD100 pKa = 3.67PLAALLPGDD109 pKa = 4.83DD110 pKa = 4.19VYY112 pKa = 11.31QGSSHH117 pKa = 7.48DD118 pKa = 4.28DD119 pKa = 3.43IIRR122 pKa = 11.84DD123 pKa = 3.75VAGHH127 pKa = 5.72NVFLGGDD134 pKa = 3.78GFDD137 pKa = 3.84QMSGGPGNDD146 pKa = 3.75HH147 pKa = 7.22LWGQSPNGGPDD158 pKa = 2.98EE159 pKa = 4.5GDD161 pKa = 3.51YY162 pKa = 11.27LAGGGGSDD170 pKa = 4.27YY171 pKa = 11.13IQGNAGDD178 pKa = 4.6DD179 pKa = 3.81LVEE182 pKa = 5.51GGDD185 pKa = 3.94GSDD188 pKa = 3.59RR189 pKa = 11.84LYY191 pKa = 11.23GGQGEE196 pKa = 4.26DD197 pKa = 3.64ALYY200 pKa = 10.61GGPGNDD206 pKa = 4.01TINGNLGNDD215 pKa = 3.95YY216 pKa = 10.92IQGNEE221 pKa = 4.06GNDD224 pKa = 3.88LLRR227 pKa = 11.84GGQGDD232 pKa = 4.63DD233 pKa = 3.13GWISGGEE240 pKa = 4.16GNDD243 pKa = 3.68TIMGDD248 pKa = 3.91LGADD252 pKa = 3.2RR253 pKa = 11.84MFGGWDD259 pKa = 3.06ADD261 pKa = 3.65LFVFGPGTSPIGATIDD277 pKa = 3.52SVADD281 pKa = 4.01FEE283 pKa = 4.75QGSDD287 pKa = 3.95LISLGFVPATVLTGSSAAAAASIDD311 pKa = 3.67AARR314 pKa = 11.84TAAQALFDD322 pKa = 3.93GHH324 pKa = 8.03AGDD327 pKa = 5.15HH328 pKa = 5.65EE329 pKa = 4.53VAAIGYY335 pKa = 9.09QGATLIFWSGNGGAAIDD352 pKa = 4.11SVVSFQNQSPTDD364 pKa = 3.6FALADD369 pKa = 4.25FGG371 pKa = 4.7

Molecular weight: 37.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8R1U6|A0A0Q8R1U6_9SPHN Abhydrolase_3 domain-containing protein OS=Sphingomonas sp. Root720 OX=1736595 GN=ASE22_08575 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5044

0

5044

1638281

29

2820

324.8

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.389 ± 0.046

0.81 ± 0.01

6.202 ± 0.027

5.231 ± 0.03

3.558 ± 0.018

9.16 ± 0.033

2.07 ± 0.017

5.234 ± 0.021

2.732 ± 0.027

9.877 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.016

2.369 ± 0.02

5.351 ± 0.025

2.905 ± 0.016

7.696 ± 0.033

5.18 ± 0.024

5.049 ± 0.025

7.098 ± 0.029

1.394 ± 0.015

2.269 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski