Amino acid dipepetide frequency for Sphingomonas sp. Root720

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.034AlaAla: 20.034 ± 0.184	1.094AlaCys: 1.094 ± 0.023	7.975AlaAsp: 7.975 ± 0.075	7.622AlaGlu: 7.622 ± 0.089	4.516AlaPhe: 4.516 ± 0.055	12.119AlaGly: 12.119 ± 0.107	2.39AlaHis: 2.39 ± 0.047	7.391AlaIle: 7.391 ± 0.08	3.635AlaLys: 3.635 ± 0.061	13.607AlaLeu: 13.607 ± 0.122	3.991AlaMet: 3.991 ± 0.057	2.857AlaAsn: 2.857 ± 0.044	6.138AlaPro: 6.138 ± 0.075	3.979AlaGln: 3.979 ± 0.054	10.291AlaArg: 10.291 ± 0.103	6.343AlaSer: 6.343 ± 0.065	6.635AlaThr: 6.635 ± 0.069	8.87AlaVal: 8.87 ± 0.091	1.668AlaTrp: 1.668 ± 0.035	2.734AlaTyr: 2.734 ± 0.041	0.001AlaXaa: 0.001 ± 0.001
Cys	1.016CysAla: 1.016 ± 0.026	0.099CysCys: 0.099 ± 0.008	0.551CysAsp: 0.551 ± 0.016	0.394CysGlu: 0.394 ± 0.018	0.344CysPhe: 0.344 ± 0.016	0.892CysGly: 0.892 ± 0.026	0.244CysHis: 0.244 ± 0.016	0.382CysIle: 0.382 ± 0.016	0.14CysLys: 0.14 ± 0.01	0.767CysLeu: 0.767 ± 0.021	0.151CysMet: 0.151 ± 0.009	0.206CysAsn: 0.206 ± 0.013	0.439CysPro: 0.439 ± 0.018	0.201CysGln: 0.201 ± 0.011	0.58CysArg: 0.58 ± 0.023	0.433CysSer: 0.433 ± 0.018	0.394CysThr: 0.394 ± 0.017	0.54CysVal: 0.54 ± 0.019	0.122CysTrp: 0.122 ± 0.009	0.204CysTyr: 0.204 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.848AspAla: 7.848 ± 0.08	0.495AspCys: 0.495 ± 0.02	3.61AspAsp: 3.61 ± 0.051	3.354AspGlu: 3.354 ± 0.047	2.24AspPhe: 2.24 ± 0.037	6.226AspGly: 6.226 ± 0.07	1.519AspHis: 1.519 ± 0.032	3.236AspIle: 3.236 ± 0.041	1.588AspLys: 1.588 ± 0.035	5.915AspLeu: 5.915 ± 0.067	1.455AspMet: 1.455 ± 0.03	1.284AspAsn: 1.284 ± 0.03	4.039AspPro: 4.039 ± 0.051	1.824AspGln: 1.824 ± 0.032	5.705AspArg: 5.705 ± 0.072	2.38AspSer: 2.38 ± 0.041	2.348AspThr: 2.348 ± 0.043	4.074AspVal: 4.074 ± 0.046	1.107AspTrp: 1.107 ± 0.027	1.771AspTyr: 1.771 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.676GluAla: 7.676 ± 0.088	0.308GluCys: 0.308 ± 0.015	2.693GluAsp: 2.693 ± 0.04	2.794GluGlu: 2.794 ± 0.053	1.318GluPhe: 1.318 ± 0.032	4.399GluGly: 4.399 ± 0.064	1.154GluHis: 1.154 ± 0.027	3.017GluIle: 3.017 ± 0.045	1.817GluLys: 1.817 ± 0.036	5.102GluLeu: 5.102 ± 0.057	1.365GluMet: 1.365 ± 0.03	1.202GluAsn: 1.202 ± 0.027	2.544GluPro: 2.544 ± 0.043	2.014GluGln: 2.014 ± 0.038	4.917GluArg: 4.917 ± 0.057	2.084GluSer: 2.084 ± 0.037	2.947GluThr: 2.947 ± 0.041	3.398GluVal: 3.398 ± 0.05	0.704GluTrp: 0.704 ± 0.02	0.853GluTyr: 0.853 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.773PheAla: 4.773 ± 0.058	0.33PheCys: 0.33 ± 0.018	2.85PheAsp: 2.85 ± 0.041	2.005PheGlu: 2.005 ± 0.036	1.237PhePhe: 1.237 ± 0.034	3.767PheGly: 3.767 ± 0.053	0.775PheHis: 0.775 ± 0.024	1.367PheIle: 1.367 ± 0.03	0.834PheLys: 0.834 ± 0.024	3.09PheLeu: 3.09 ± 0.05	0.7PheMet: 0.7 ± 0.021	1.018PheAsn: 1.018 ± 0.03	1.549PhePro: 1.549 ± 0.03	0.914PheGln: 0.914 ± 0.024	2.376PheArg: 2.376 ± 0.037	1.994PheSer: 1.994 ± 0.036	1.972PheThr: 1.972 ± 0.037	2.647PheVal: 2.647 ± 0.045	0.472PheTrp: 0.472 ± 0.016	0.916PheTyr: 0.916 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	10.414GlyAla: 10.414 ± 0.084	0.877GlyCys: 0.877 ± 0.024	5.315GlyAsp: 5.315 ± 0.069	4.804GlyGlu: 4.804 ± 0.053	3.874GlyPhe: 3.874 ± 0.052	8.726GlyGly: 8.726 ± 0.136	1.976GlyHis: 1.976 ± 0.035	4.728GlyIle: 4.728 ± 0.051	2.952GlyLys: 2.952 ± 0.05	9.148GlyLeu: 9.148 ± 0.082	2.306GlyMet: 2.306 ± 0.04	2.189GlyAsn: 2.189 ± 0.053	3.974GlyPro: 3.974 ± 0.044	2.891GlyGln: 2.891 ± 0.043	7.282GlyArg: 7.282 ± 0.07	4.983GlySer: 4.983 ± 0.068	4.735GlyThr: 4.735 ± 0.072	6.239GlyVal: 6.239 ± 0.072	1.687GlyTrp: 1.687 ± 0.032	2.499GlyTyr: 2.499 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.44HisAla: 2.44 ± 0.042	0.241HisCys: 0.241 ± 0.01	1.277HisAsp: 1.277 ± 0.029	0.956HisGlu: 0.956 ± 0.022	0.831HisPhe: 0.831 ± 0.026	2.217HisGly: 2.217 ± 0.048	0.585HisHis: 0.585 ± 0.019	1.021HisIle: 1.021 ± 0.027	0.417HisLys: 0.417 ± 0.018	2.004HisLeu: 2.004 ± 0.037	0.497HisMet: 0.497 ± 0.02	0.439HisAsn: 0.439 ± 0.015	1.379HisPro: 1.379 ± 0.035	0.533HisGln: 0.533 ± 0.02	1.677HisArg: 1.677 ± 0.033	0.967HisSer: 0.967 ± 0.025	0.587HisThr: 0.587 ± 0.019	1.651HisVal: 1.651 ± 0.029	0.378HisTrp: 0.378 ± 0.014	0.605HisTyr: 0.605 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	8.123IleAla: 8.123 ± 0.078	0.472IleCys: 0.472 ± 0.015	4.375IleAsp: 4.375 ± 0.051	3.702IleGlu: 3.702 ± 0.047	1.664IlePhe: 1.664 ± 0.031	5.506IleGly: 5.506 ± 0.063	0.949IleHis: 0.949 ± 0.024	1.984IleIle: 1.984 ± 0.041	1.137IleLys: 1.137 ± 0.032	4.195IleLeu: 4.195 ± 0.054	0.894IleMet: 0.894 ± 0.025	1.303IleAsn: 1.303 ± 0.03	2.22IlePro: 2.22 ± 0.037	1.163IleGln: 1.163 ± 0.03	3.463IleArg: 3.463 ± 0.044	2.744IleSer: 2.744 ± 0.038	2.365IleThr: 2.365 ± 0.037	4.451IleVal: 4.451 ± 0.052	0.593IleTrp: 0.593 ± 0.022	1.04IleTyr: 1.04 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	3.86LysAla: 3.86 ± 0.056	0.142LysCys: 0.142 ± 0.01	1.437LysAsp: 1.437 ± 0.033	1.055LysGlu: 1.055 ± 0.027	0.725LysPhe: 0.725 ± 0.022	2.508LysGly: 2.508 ± 0.038	0.437LysHis: 0.437 ± 0.017	1.452LysIle: 1.452 ± 0.031	0.886LysLys: 0.886 ± 0.032	3.007LysLeu: 3.007 ± 0.049	0.695LysMet: 0.695 ± 0.02	0.62LysAsn: 0.62 ± 0.021	1.848LysPro: 1.848 ± 0.041	0.78LysGln: 0.78 ± 0.026	2.066LysArg: 2.066 ± 0.039	1.369LysSer: 1.369 ± 0.036	1.495LysThr: 1.495 ± 0.034	2.07LysVal: 2.07 ± 0.041	0.321LysTrp: 0.321 ± 0.015	0.547LysTyr: 0.547 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	13.899LeuAla: 13.899 ± 0.142	0.87LeuCys: 0.87 ± 0.025	6.595LeuAsp: 6.595 ± 0.07	4.568LeuGlu: 4.568 ± 0.059	3.696LeuPhe: 3.696 ± 0.057	8.524LeuGly: 8.524 ± 0.088	1.959LeuHis: 1.959 ± 0.038	4.98LeuIle: 4.98 ± 0.063	2.802LeuLys: 2.802 ± 0.052	9.749LeuLeu: 9.749 ± 0.116	2.101LeuMet: 2.101 ± 0.04	2.262LeuAsn: 2.262 ± 0.034	5.617LeuPro: 5.617 ± 0.063	2.376LeuGln: 2.376 ± 0.044	7.198LeuArg: 7.198 ± 0.076	6.02LeuSer: 6.02 ± 0.066	5.22LeuThr: 5.22 ± 0.064	7.127LeuVal: 7.127 ± 0.087	1.233LeuTrp: 1.233 ± 0.028	1.972LeuTyr: 1.972 ± 0.034	0.001LeuXaa: 0.001 ± 0.001
Met	3.402MetAla: 3.402 ± 0.051	0.157MetCys: 0.157 ± 0.009	1.086MetAsp: 1.086 ± 0.027	0.959MetGlu: 0.959 ± 0.024	0.69MetPhe: 0.69 ± 0.02	1.795MetGly: 1.795 ± 0.035	0.45MetHis: 0.45 ± 0.016	1.507MetIle: 1.507 ± 0.03	0.831MetLys: 0.831 ± 0.024	2.73MetLeu: 2.73 ± 0.044	0.651MetMet: 0.651 ± 0.021	0.61MetAsn: 0.61 ± 0.022	1.456MetPro: 1.456 ± 0.032	0.635MetGln: 0.635 ± 0.02	1.858MetArg: 1.858 ± 0.037	1.477MetSer: 1.477 ± 0.035	1.758MetThr: 1.758 ± 0.033	1.671MetVal: 1.671 ± 0.032	0.228MetTrp: 0.228 ± 0.013	0.284MetTyr: 0.284 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.038AsnAla: 3.038 ± 0.044	0.23AsnCys: 0.23 ± 0.011	1.358AsnAsp: 1.358 ± 0.04	0.97AsnGlu: 0.97 ± 0.025	0.856AsnPhe: 0.856 ± 0.028	2.28AsnGly: 2.28 ± 0.04	0.458AsnHis: 0.458 ± 0.018	1.356AsnIle: 1.356 ± 0.032	0.557AsnLys: 0.557 ± 0.021	2.345AsnLeu: 2.345 ± 0.043	0.557AsnMet: 0.557 ± 0.02	0.636AsnAsn: 0.636 ± 0.024	1.577AsnPro: 1.577 ± 0.031	0.693AsnGln: 0.693 ± 0.022	1.81AsnArg: 1.81 ± 0.039	1.179AsnSer: 1.179 ± 0.032	0.97AsnThr: 0.97 ± 0.031	1.781AsnVal: 1.781 ± 0.039	0.398AsnTrp: 0.398 ± 0.017	0.643AsnTyr: 0.643 ± 0.02	0.001AsnXaa: 0.001 ± 0.001
Pro	7.324ProAla: 7.324 ± 0.076	0.335ProCys: 0.335 ± 0.016	3.9ProAsp: 3.9 ± 0.053	3.251ProGlu: 3.251 ± 0.05	1.94ProPhe: 1.94 ± 0.034	5.066ProGly: 5.066 ± 0.063	1.105ProHis: 1.105 ± 0.028	2.704ProIle: 2.704 ± 0.041	1.428ProLys: 1.428 ± 0.032	4.992ProLeu: 4.992 ± 0.059	1.251ProMet: 1.251 ± 0.026	1.215ProAsn: 1.215 ± 0.028	2.915ProPro: 2.915 ± 0.058	1.488ProGln: 1.488 ± 0.029	3.25ProArg: 3.25 ± 0.051	2.749ProSer: 2.749 ± 0.041	2.594ProThr: 2.594 ± 0.04	4.223ProVal: 4.223 ± 0.058	0.678ProTrp: 0.678 ± 0.022	1.102ProTyr: 1.102 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	4.146GlnAla: 4.146 ± 0.06	0.211GlnCys: 0.211 ± 0.011	1.385GlnAsp: 1.385 ± 0.03	1.211GlnGlu: 1.211 ± 0.023	1.019GlnPhe: 1.019 ± 0.026	2.438GlnGly: 2.438 ± 0.04	0.565GlnHis: 0.565 ± 0.021	1.631GlnIle: 1.631 ± 0.037	0.81GlnLys: 0.81 ± 0.022	2.945GlnLeu: 2.945 ± 0.042	0.718GlnMet: 0.718 ± 0.018	0.718GlnAsn: 0.718 ± 0.025	1.759GlnPro: 1.759 ± 0.032	1.063GlnGln: 1.063 ± 0.028	2.415GlnArg: 2.415 ± 0.043	1.558GlnSer: 1.558 ± 0.032	1.453GlnThr: 1.453 ± 0.03	2.057GlnVal: 2.057 ± 0.031	0.389GlnTrp: 0.389 ± 0.017	0.563GlnTyr: 0.563 ± 0.022	0.002GlnXaa: 0.002 ± 0.001
Arg	9.186ArgAla: 9.186 ± 0.089	0.58ArgCys: 0.58 ± 0.02	4.578ArgAsp: 4.578 ± 0.063	3.896ArgGlu: 3.896 ± 0.06	3.271ArgPhe: 3.271 ± 0.038	5.455ArgGly: 5.455 ± 0.058	1.868ArgHis: 1.868 ± 0.035	4.521ArgIle: 4.521 ± 0.053	2.03ArgLys: 2.03 ± 0.042	8.694ArgLeu: 8.694 ± 0.097	2.037ArgMet: 2.037 ± 0.036	1.842ArgAsn: 1.842 ± 0.037	4.142ArgPro: 4.142 ± 0.059	2.551ArgGln: 2.551 ± 0.044	6.549ArgArg: 6.549 ± 0.08	4.093ArgSer: 4.093 ± 0.059	3.709ArgThr: 3.709 ± 0.043	4.679ArgVal: 4.679 ± 0.056	1.306ArgTrp: 1.306 ± 0.031	1.977ArgTyr: 1.977 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.329SerAla: 6.329 ± 0.066	0.414SerCys: 0.414 ± 0.019	3.09SerAsp: 3.09 ± 0.046	2.371SerGlu: 2.371 ± 0.043	2.27SerPhe: 2.27 ± 0.039	5.388SerGly: 5.388 ± 0.081	1.024SerHis: 1.024 ± 0.027	2.867SerIle: 2.867 ± 0.046	1.307SerLys: 1.307 ± 0.029	5.053SerLeu: 5.053 ± 0.061	1.196SerMet: 1.196 ± 0.029	1.285SerAsn: 1.285 ± 0.036	2.918SerPro: 2.918 ± 0.04	1.401SerGln: 1.401 ± 0.031	3.516SerArg: 3.516 ± 0.048	2.811SerSer: 2.811 ± 0.051	2.656SerThr: 2.656 ± 0.049	3.664SerVal: 3.664 ± 0.045	0.767SerTrp: 0.767 ± 0.023	1.478SerTyr: 1.478 ± 0.033	0.001SerXaa: 0.001 ± 0.001
Thr	6.37ThrAla: 6.37 ± 0.066	0.354ThrCys: 0.354 ± 0.015	2.92ThrAsp: 2.92 ± 0.043	2.159ThrGlu: 2.159 ± 0.037	1.632ThrPhe: 1.632 ± 0.035	5.271ThrGly: 5.271 ± 0.072	0.925ThrHis: 0.925 ± 0.027	3.093ThrIle: 3.093 ± 0.049	1.185ThrLys: 1.185 ± 0.03	5.41ThrLeu: 5.41 ± 0.066	1.231ThrMet: 1.231 ± 0.029	1.159ThrAsn: 1.159 ± 0.031	3.258ThrPro: 3.258 ± 0.054	1.296ThrGln: 1.296 ± 0.03	3.276ThrArg: 3.276 ± 0.047	2.606ThrSer: 2.606 ± 0.049	2.606ThrThr: 2.606 ± 0.049	4.037ThrVal: 4.037 ± 0.055	0.541ThrTrp: 0.541 ± 0.02	1.157ThrTyr: 1.157 ± 0.029	0.002ThrXaa: 0.002 ± 0.002
Val	9.925ValAla: 9.925 ± 0.088	0.546ValCys: 0.546 ± 0.018	4.74ValAsp: 4.74 ± 0.053	4.5ValGlu: 4.5 ± 0.049	2.064ValPhe: 2.064 ± 0.041	5.798ValGly: 5.798 ± 0.073	1.416ValHis: 1.416 ± 0.033	3.516ValIle: 3.516 ± 0.051	1.884ValLys: 1.884 ± 0.037	6.196ValLeu: 6.196 ± 0.068	1.629ValMet: 1.629 ± 0.031	1.801ValAsn: 1.801 ± 0.035	3.96ValPro: 3.96 ± 0.055	2.001ValGln: 2.001 ± 0.032	5.34ValArg: 5.34 ± 0.063	3.895ValSer: 3.895 ± 0.061	4.222ValThr: 4.222 ± 0.052	5.326ValVal: 5.326 ± 0.071	0.794ValTrp: 0.794 ± 0.024	1.425ValTyr: 1.425 ± 0.033	0.001ValXaa: 0.001 ± 0.001
Trp	1.333TrpAla: 1.333 ± 0.029	0.124TrpCys: 0.124 ± 0.009	0.729TrpAsp: 0.729 ± 0.026	0.569TrpGlu: 0.569 ± 0.018	0.522TrpPhe: 0.522 ± 0.019	0.952TrpGly: 0.952 ± 0.026	0.379TrpHis: 0.379 ± 0.017	0.716TrpIle: 0.716 ± 0.021	0.488TrpLys: 0.488 ± 0.021	1.702TrpLeu: 1.702 ± 0.038	0.353TrpMet: 0.353 ± 0.014	0.446TrpAsn: 0.446 ± 0.016	0.721TrpPro: 0.721 ± 0.021	0.526TrpGln: 0.526 ± 0.021	1.382TrpArg: 1.382 ± 0.031	0.892TrpSer: 0.892 ± 0.024	0.825TrpThr: 0.825 ± 0.026	0.79TrpVal: 0.79 ± 0.023	0.212TrpTrp: 0.212 ± 0.011	0.274TrpTyr: 0.274 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.757TyrAla: 2.757 ± 0.041	0.22TyrCys: 0.22 ± 0.012	1.552TyrAsp: 1.552 ± 0.033	1.162TyrGlu: 1.162 ± 0.028	0.876TyrPhe: 0.876 ± 0.025	2.273TyrGly: 2.273 ± 0.041	0.526TyrHis: 0.526 ± 0.018	0.877TyrIle: 0.877 ± 0.024	0.59TyrLys: 0.59 ± 0.022	2.117TyrLeu: 2.117 ± 0.036	0.457TyrMet: 0.457 ± 0.016	0.6TyrAsn: 0.6 ± 0.017	1.058TyrPro: 1.058 ± 0.027	0.725TyrGln: 0.725 ± 0.022	2.014TyrArg: 2.014 ± 0.035	1.226TyrSer: 1.226 ± 0.033	0.995TyrThr: 0.995 ± 0.027	1.682TyrVal: 1.682 ± 0.036	0.338TyrTrp: 0.338 ± 0.014	0.651TyrTyr: 0.651 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5044 proteins (1638281 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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