Adhaeribacter soli
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3831 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N1INY4|A0A5N1INY4_9BACT DNA repair protein RecN OS=Adhaeribacter soli OX=2607655 GN=recN PE=3 SV=1
MM1 pKa = 7.33 IWITGNIEE9 pKa = 3.76 LLKK12 pKa = 10.84 ASDD15 pKa = 3.93 YY16 pKa = 10.89 IIYY19 pKa = 7.92 NTTKK23 pKa = 10.07 YY24 pKa = 10.51 RR25 pKa = 11.84 SAQEE29 pKa = 4.37 TEE31 pKa = 3.84 VHH33 pKa = 6.58 DD34 pKa = 4.07 PVIISGDD41 pKa = 3.63 NLIMDD46 pKa = 4.4 EE47 pKa = 4.49 EE48 pKa = 4.53 KK49 pKa = 11.24 SNTSDD54 pKa = 5.38 DD55 pKa = 3.68 DD56 pKa = 3.84 TGNNGQLYY64 pKa = 9.56 YY65 pKa = 10.08 EE66 pKa = 3.96 FHH68 pKa = 6.41 VLSEE72 pKa = 4.42 GEE74 pKa = 3.86 PTDD77 pKa = 3.22 RR78 pKa = 11.84 VLIVYY83 pKa = 10.12 HH84 pKa = 5.81 DD85 pKa = 4.18 TIKK88 pKa = 11.07 NEE90 pKa = 3.95 YY91 pKa = 8.8 TGWCAEE97 pKa = 4.25 DD98 pKa = 3.23
Molecular weight: 11.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.854
IPC2_protein 4.113
IPC_protein 4.05
Toseland 3.859
ProMoST 4.113
Dawson 4.012
Bjellqvist 4.215
Wikipedia 3.923
Rodwell 3.884
Grimsley 3.77
Solomon 4.012
Lehninger 3.961
Nozaki 4.139
DTASelect 4.317
Thurlkill 3.897
EMBOSS 3.935
Sillero 4.164
Patrickios 0.998
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.081
Protein with the highest isoelectric point:
>tr|A0A5N1IX26|A0A5N1IX26_9BACT Uncharacterized protein OS=Adhaeribacter soli OX=2607655 GN=F0P94_08605 PE=4 SV=1
MM1 pKa = 7.33 LCYY4 pKa = 10.52 RR5 pKa = 11.84 NMQDD9 pKa = 2.88 FCSIGMRR16 pKa = 11.84 FMANQIYY23 pKa = 9.87 QPTKK27 pKa = 8.92 VAGTRR32 pKa = 11.84 MLFTMTMTMIVVMAMTCFLMRR53 pKa = 11.84 AMVVMMWQHH62 pKa = 5.47 NMRR65 pKa = 11.84 LHH67 pKa = 4.33 YY68 pKa = 10.66 HH69 pKa = 6.19 KK70 pKa = 10.79 GKK72 pKa = 10.28 KK73 pKa = 8.69 KK74 pKa = 10.24 KK75 pKa = 10.12 KK76 pKa = 8.98 RR77 pKa = 3.49
Molecular weight: 9.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.955
IPC_protein 10.54
Toseland 10.804
ProMoST 10.672
Dawson 10.891
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.316
Grimsley 10.935
Solomon 10.965
Lehninger 10.95
Nozaki 10.789
DTASelect 10.555
Thurlkill 10.789
EMBOSS 11.184
Sillero 10.818
Patrickios 11.082
IPC_peptide 10.979
IPC2_peptide 9.663
IPC2.peptide.svr19 8.403
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3831
0
3831
1363969
26
4629
356.0
39.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.957 ± 0.043
0.799 ± 0.016
4.689 ± 0.03
6.052 ± 0.059
4.967 ± 0.034
7.027 ± 0.052
1.774 ± 0.02
6.324 ± 0.039
6.412 ± 0.058
9.837 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.141 ± 0.025
5.554 ± 0.048
4.303 ± 0.03
3.956 ± 0.031
4.152 ± 0.033
6.25 ± 0.05
6.366 ± 0.083
6.494 ± 0.034
1.122 ± 0.015
3.824 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here