Rhizobiales bacterium NRL2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; unclassified Hyphomicrobiales

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4302 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A192INC7|A0A192INC7_9RHIZ DUF1254 domain-containing protein OS=Rhizobiales bacterium NRL2 OX=1862950 GN=TEF_19105 PE=4 SV=1
MM1 pKa = 7.14RR2 pKa = 11.84TLIIVMGLTALVSFGARR19 pKa = 11.84AEE21 pKa = 3.95NLYY24 pKa = 10.74FLLINQSSADD34 pKa = 3.03IVEE37 pKa = 4.57FHH39 pKa = 6.8VSPPDD44 pKa = 3.38ASRR47 pKa = 11.84WTDD50 pKa = 3.13NLIPDD55 pKa = 5.07GYY57 pKa = 10.84VLPAGNEE64 pKa = 3.89VEE66 pKa = 4.42VAIEE70 pKa = 4.74DD71 pKa = 4.29GQDD74 pKa = 2.67HH75 pKa = 6.73CAYY78 pKa = 10.49DD79 pKa = 3.58IRR81 pKa = 11.84AVFADD86 pKa = 3.52GEE88 pKa = 4.5VFEE91 pKa = 5.89EE92 pKa = 4.82YY93 pKa = 9.39DD94 pKa = 3.37TDD96 pKa = 4.39LCEE99 pKa = 4.31LGQWTFTDD107 pKa = 3.39

Molecular weight: 11.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A192III0|A0A192III0_9RHIZ Membrane protein insertase YidC OS=Rhizobiales bacterium NRL2 OX=1862950 GN=yidC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.51GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.89ATVGGLRR29 pKa = 11.84ILQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.89GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4302

0

4302

1392434

41

6886

323.7

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.626 ± 0.051

0.854 ± 0.013

6.401 ± 0.068

6.531 ± 0.039

3.672 ± 0.021

9.237 ± 0.083

2.085 ± 0.02

4.887 ± 0.023

2.763 ± 0.03

9.737 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.02

2.427 ± 0.026

5.099 ± 0.035

2.806 ± 0.022

7.857 ± 0.06

4.596 ± 0.024

4.818 ± 0.026

7.408 ± 0.029

1.378 ± 0.015

2.172 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski