Amino acid dipepetide frequency for Rhizobiales bacterium NRL2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.538AlaAla: 18.538 ± 0.186
1.102AlaCys: 1.102 ± 0.029
8.014AlaAsp: 8.014 ± 0.115
9.499AlaGlu: 9.499 ± 0.12
4.467AlaPhe: 4.467 ± 0.062
12.273AlaGly: 12.273 ± 0.179
2.247AlaHis: 2.247 ± 0.04
5.895AlaIle: 5.895 ± 0.076
3.248AlaLys: 3.248 ± 0.062
12.686AlaLeu: 12.686 ± 0.118
3.739AlaMet: 3.739 ± 0.054
2.73AlaAsn: 2.73 ± 0.055
5.442AlaPro: 5.442 ± 0.082
3.268AlaGln: 3.268 ± 0.058
9.526AlaArg: 9.526 ± 0.118
4.899AlaSer: 4.899 ± 0.062
5.353AlaThr: 5.353 ± 0.055
9.269AlaVal: 9.269 ± 0.094
1.624AlaTrp: 1.624 ± 0.034
2.443AlaTyr: 2.443 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
0.965CysAla: 0.965 ± 0.031
0.117CysCys: 0.117 ± 0.011
0.565CysAsp: 0.565 ± 0.021
0.446CysGlu: 0.446 ± 0.019
0.366CysPhe: 0.366 ± 0.019
0.971CysGly: 0.971 ± 0.029
0.279CysHis: 0.279 ± 0.019
0.396CysIle: 0.396 ± 0.018
0.146CysLys: 0.146 ± 0.01
0.803CysLeu: 0.803 ± 0.024
0.21CysMet: 0.21 ± 0.012
0.209CysAsn: 0.209 ± 0.013
0.463CysPro: 0.463 ± 0.019
0.226CysGln: 0.226 ± 0.013
0.723CysArg: 0.723 ± 0.023
0.37CysSer: 0.37 ± 0.018
0.376CysThr: 0.376 ± 0.019
0.591CysVal: 0.591 ± 0.022
0.113CysTrp: 0.113 ± 0.009
0.208CysTyr: 0.208 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.378AspAla: 7.378 ± 0.081
0.58AspCys: 0.58 ± 0.022
4.304AspAsp: 4.304 ± 0.158
4.058AspGlu: 4.058 ± 0.057
2.476AspPhe: 2.476 ± 0.05
6.474AspGly: 6.474 ± 0.175
1.453AspHis: 1.453 ± 0.033
3.226AspIle: 3.226 ± 0.052
1.414AspLys: 1.414 ± 0.034
6.419AspLeu: 6.419 ± 0.083
1.668AspMet: 1.668 ± 0.033
1.309AspAsn: 1.309 ± 0.038
3.784AspPro: 3.784 ± 0.057
1.652AspGln: 1.652 ± 0.039
5.432AspArg: 5.432 ± 0.07
2.366AspSer: 2.366 ± 0.081
2.754AspThr: 2.754 ± 0.169
4.417AspVal: 4.417 ± 0.065
1.185AspTrp: 1.185 ± 0.034
1.658AspTyr: 1.658 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
8.667GluAla: 8.667 ± 0.099
0.402GluCys: 0.402 ± 0.018
3.525GluAsp: 3.525 ± 0.049
3.783GluGlu: 3.783 ± 0.063
1.797GluPhe: 1.797 ± 0.04
5.284GluGly: 5.284 ± 0.079
1.331GluHis: 1.331 ± 0.03
3.992GluIle: 3.992 ± 0.062
2.385GluLys: 2.385 ± 0.038
6.083GluLeu: 6.083 ± 0.073
1.932GluMet: 1.932 ± 0.04
1.795GluAsn: 1.795 ± 0.037
3.187GluPro: 3.187 ± 0.055
2.16GluGln: 2.16 ± 0.05
5.936GluArg: 5.936 ± 0.074
2.794GluSer: 2.794 ± 0.046
4.05GluThr: 4.05 ± 0.059
4.349GluVal: 4.349 ± 0.063
0.757GluTrp: 0.757 ± 0.026
1.102GluTyr: 1.102 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.448PheAla: 4.448 ± 0.064
0.448PheCys: 0.448 ± 0.018
2.788PheAsp: 2.788 ± 0.046
2.439PheGlu: 2.439 ± 0.049
1.338PhePhe: 1.338 ± 0.032
3.958PheGly: 3.958 ± 0.076
0.859PheHis: 0.859 ± 0.024
1.564PheIle: 1.564 ± 0.037
0.666PheLys: 0.666 ± 0.022
3.25PheLeu: 3.25 ± 0.062
0.829PheMet: 0.829 ± 0.024
0.909PheAsn: 0.909 ± 0.028
1.539PhePro: 1.539 ± 0.033
0.957PheGln: 0.957 ± 0.026
2.845PheArg: 2.845 ± 0.045
1.875PheSer: 1.875 ± 0.038
1.818PheThr: 1.818 ± 0.037
2.83PheVal: 2.83 ± 0.044
0.51PheTrp: 0.51 ± 0.019
0.85PheTyr: 0.85 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
10.324GlyAla: 10.324 ± 0.186
0.848GlyCys: 0.848 ± 0.023
5.908GlyAsp: 5.908 ± 0.233
6.108GlyGlu: 6.108 ± 0.071
3.936GlyPhe: 3.936 ± 0.058
9.196GlyGly: 9.196 ± 0.303
2.086GlyHis: 2.086 ± 0.04
4.163GlyIle: 4.163 ± 0.059
2.557GlyLys: 2.557 ± 0.051
9.101GlyLeu: 9.101 ± 0.091
2.616GlyMet: 2.616 ± 0.055
2.251GlyAsn: 2.251 ± 0.118
3.996GlyPro: 3.996 ± 0.06
2.833GlyGln: 2.833 ± 0.055
7.658GlyArg: 7.658 ± 0.091
4.097GlySer: 4.097 ± 0.089
3.969GlyThr: 3.969 ± 0.083
6.909GlyVal: 6.909 ± 0.083
1.486GlyTrp: 1.486 ± 0.036
2.327GlyTyr: 2.327 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.251HisAla: 2.251 ± 0.041
0.246HisCys: 0.246 ± 0.014
1.408HisAsp: 1.408 ± 0.034
1.251HisGlu: 1.251 ± 0.035
0.832HisPhe: 0.832 ± 0.022
2.165HisGly: 2.165 ± 0.041
0.562HisHis: 0.562 ± 0.025
0.89HisIle: 0.89 ± 0.024
0.427HisLys: 0.427 ± 0.02
2.089HisLeu: 2.089 ± 0.042
0.513HisMet: 0.513 ± 0.018
0.451HisAsn: 0.451 ± 0.019
1.387HisPro: 1.387 ± 0.029
0.556HisGln: 0.556 ± 0.022
1.637HisArg: 1.637 ± 0.038
0.848HisSer: 0.848 ± 0.029
0.775HisThr: 0.775 ± 0.025
1.633HisVal: 1.633 ± 0.037
0.378HisTrp: 0.378 ± 0.015
0.556HisTyr: 0.556 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
6.808IleAla: 6.808 ± 0.069
0.517IleCys: 0.517 ± 0.019
3.721IleAsp: 3.721 ± 0.064
3.745IleGlu: 3.745 ± 0.055
1.647IlePhe: 1.647 ± 0.039
5.063IleGly: 5.063 ± 0.071
0.956IleHis: 0.956 ± 0.026
1.887IleIle: 1.887 ± 0.042
1.018IleLys: 1.018 ± 0.028
4.035IleLeu: 4.035 ± 0.058
0.982IleMet: 0.982 ± 0.028
1.25IleAsn: 1.25 ± 0.029
2.042IlePro: 2.042 ± 0.043
1.129IleGln: 1.129 ± 0.024
3.608IleArg: 3.608 ± 0.06
2.408IleSer: 2.408 ± 0.045
2.221IleThr: 2.221 ± 0.045
4.074IleVal: 4.074 ± 0.059
0.682IleTrp: 0.682 ± 0.025
1.077IleTyr: 1.077 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
3.539LysAla: 3.539 ± 0.053
0.168LysCys: 0.168 ± 0.012
1.527LysAsp: 1.527 ± 0.041
1.495LysGlu: 1.495 ± 0.038
0.812LysPhe: 0.812 ± 0.026
2.42LysGly: 2.42 ± 0.043
0.501LysHis: 0.501 ± 0.018
1.373LysIle: 1.373 ± 0.032
0.995LysLys: 0.995 ± 0.036
2.738LysLeu: 2.738 ± 0.051
0.703LysMet: 0.703 ± 0.025
0.669LysAsn: 0.669 ± 0.023
1.737LysPro: 1.737 ± 0.04
0.837LysGln: 0.837 ± 0.024
2.275LysArg: 2.275 ± 0.038
1.416LysSer: 1.416 ± 0.03
1.512LysThr: 1.512 ± 0.035
2.04LysVal: 2.04 ± 0.046
0.319LysTrp: 0.319 ± 0.016
0.551LysTyr: 0.551 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
13.333LeuAla: 13.333 ± 0.125
0.825LeuCys: 0.825 ± 0.026
6.43LeuAsp: 6.43 ± 0.084
5.995LeuGlu: 5.995 ± 0.07
3.475LeuPhe: 3.475 ± 0.054
8.459LeuGly: 8.459 ± 0.095
1.878LeuHis: 1.878 ± 0.038
4.851LeuIle: 4.851 ± 0.067
3.392LeuLys: 3.392 ± 0.053
8.836LeuLeu: 8.836 ± 0.101
2.353LeuMet: 2.353 ± 0.046
2.437LeuAsn: 2.437 ± 0.04
5.15LeuPro: 5.15 ± 0.076
2.542LeuGln: 2.542 ± 0.043
6.745LeuArg: 6.745 ± 0.09
5.494LeuSer: 5.494 ± 0.069
5.172LeuThr: 5.172 ± 0.065
6.885LeuVal: 6.885 ± 0.075
1.177LeuTrp: 1.177 ± 0.035
1.941LeuTyr: 1.941 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
3.518MetAla: 3.518 ± 0.059
0.173MetCys: 0.173 ± 0.012
1.396MetAsp: 1.396 ± 0.033
1.394MetGlu: 1.394 ± 0.037
0.792MetPhe: 0.792 ± 0.024
2.027MetGly: 2.027 ± 0.047
0.46MetHis: 0.46 ± 0.019
1.403MetIle: 1.403 ± 0.035
1.027MetLys: 1.027 ± 0.032
2.626MetLeu: 2.626 ± 0.042
0.758MetMet: 0.758 ± 0.029
0.835MetAsn: 0.835 ± 0.025
1.481MetPro: 1.481 ± 0.033
0.832MetGln: 0.832 ± 0.026
1.861MetArg: 1.861 ± 0.041
1.629MetSer: 1.629 ± 0.03
1.898MetThr: 1.898 ± 0.038
1.766MetVal: 1.766 ± 0.038
0.207MetTrp: 0.207 ± 0.012
0.368MetTyr: 0.368 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.013AsnAla: 3.013 ± 0.05
0.254AsnCys: 0.254 ± 0.015
1.608AsnAsp: 1.608 ± 0.107
1.288AsnGlu: 1.288 ± 0.032
0.857AsnPhe: 0.857 ± 0.027
2.234AsnGly: 2.234 ± 0.058
0.511AsnHis: 0.511 ± 0.021
1.158AsnIle: 1.158 ± 0.028
0.513AsnLys: 0.513 ± 0.021
2.448AsnLeu: 2.448 ± 0.041
0.634AsnMet: 0.634 ± 0.02
0.618AsnAsn: 0.618 ± 0.024
1.688AsnPro: 1.688 ± 0.042
0.661AsnGln: 0.661 ± 0.022
1.894AsnArg: 1.894 ± 0.037
0.962AsnSer: 0.962 ± 0.026
1.066AsnThr: 1.066 ± 0.03
1.826AsnVal: 1.826 ± 0.038
0.414AsnTrp: 0.414 ± 0.017
0.62AsnTyr: 0.62 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
6.433ProAla: 6.433 ± 0.092
0.312ProCys: 0.312 ± 0.015
4.04ProAsp: 4.04 ± 0.061
4.501ProGlu: 4.501 ± 0.069
1.867ProPhe: 1.867 ± 0.04
4.869ProGly: 4.869 ± 0.071
0.972ProHis: 0.972 ± 0.026
1.97ProIle: 1.97 ± 0.036
1.62ProLys: 1.62 ± 0.041
4.555ProLeu: 4.555 ± 0.06
1.263ProMet: 1.263 ± 0.029
1.162ProAsn: 1.162 ± 0.029
2.674ProPro: 2.674 ± 0.065
1.301ProGln: 1.301 ± 0.032
3.01ProArg: 3.01 ± 0.049
2.3ProSer: 2.3 ± 0.04
2.101ProThr: 2.101 ± 0.04
4.24ProVal: 4.24 ± 0.062
0.712ProTrp: 0.712 ± 0.023
1.087ProTyr: 1.087 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.865GlnAla: 3.865 ± 0.062
0.193GlnCys: 0.193 ± 0.013
1.425GlnAsp: 1.425 ± 0.029
1.456GlnGlu: 1.456 ± 0.031
0.947GlnPhe: 0.947 ± 0.024
2.333GlnGly: 2.333 ± 0.05
0.542GlnHis: 0.542 ± 0.017
1.525GlnIle: 1.525 ± 0.037
0.938GlnLys: 0.938 ± 0.03
2.358GlnLeu: 2.358 ± 0.042
0.845GlnMet: 0.845 ± 0.027
0.748GlnAsn: 0.748 ± 0.022
1.579GlnPro: 1.579 ± 0.033
1.046GlnGln: 1.046 ± 0.033
2.37GlnArg: 2.37 ± 0.05
1.438GlnSer: 1.438 ± 0.037
1.43GlnThr: 1.43 ± 0.036
2.134GlnVal: 2.134 ± 0.038
0.381GlnTrp: 0.381 ± 0.016
0.509GlnTyr: 0.509 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
8.849ArgAla: 8.849 ± 0.114
0.564ArgCys: 0.564 ± 0.022
4.686ArgAsp: 4.686 ± 0.058
5.105ArgGlu: 5.105 ± 0.069
3.188ArgPhe: 3.188 ± 0.053
5.525ArgGly: 5.525 ± 0.059
1.996ArgHis: 1.996 ± 0.043
4.304ArgIle: 4.304 ± 0.06
2.183ArgLys: 2.183 ± 0.049
8.782ArgLeu: 8.782 ± 0.113
2.16ArgMet: 2.16 ± 0.043
1.861ArgAsn: 1.861 ± 0.033
4.096ArgPro: 4.096 ± 0.067
2.705ArgGln: 2.705 ± 0.048
7.485ArgArg: 7.485 ± 0.098
3.425ArgSer: 3.425 ± 0.051
3.518ArgThr: 3.518 ± 0.059
5.219ArgVal: 5.219 ± 0.079
1.114ArgTrp: 1.114 ± 0.028
1.808ArgTyr: 1.808 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.41SerAla: 5.41 ± 0.074
0.373SerCys: 0.373 ± 0.018
2.858SerAsp: 2.858 ± 0.06
2.809SerGlu: 2.809 ± 0.045
1.956SerPhe: 1.956 ± 0.037
5.171SerGly: 5.171 ± 0.09
1.016SerHis: 1.016 ± 0.029
2.271SerIle: 2.271 ± 0.045
1.172SerLys: 1.172 ± 0.033
4.384SerLeu: 4.384 ± 0.065
1.221SerMet: 1.221 ± 0.029
1.024SerAsn: 1.024 ± 0.027
2.372SerPro: 2.372 ± 0.039
1.222SerGln: 1.222 ± 0.034
3.306SerArg: 3.306 ± 0.053
2.128SerSer: 2.128 ± 0.049
2.04SerThr: 2.04 ± 0.041
3.522SerVal: 3.522 ± 0.052
0.618SerTrp: 0.618 ± 0.02
1.09SerTyr: 1.09 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
6.137ThrAla: 6.137 ± 0.068
0.395ThrCys: 0.395 ± 0.018
2.905ThrAsp: 2.905 ± 0.067
2.926ThrGlu: 2.926 ± 0.053
1.722ThrPhe: 1.722 ± 0.034
5.101ThrGly: 5.101 ± 0.092
0.861ThrHis: 0.861 ± 0.024
2.498ThrIle: 2.498 ± 0.057
1.065ThrLys: 1.065 ± 0.03
4.834ThrLeu: 4.834 ± 0.108
1.151ThrMet: 1.151 ± 0.031
1.077ThrAsn: 1.077 ± 0.03
3.045ThrPro: 3.045 ± 0.043
1.088ThrGln: 1.088 ± 0.026
3.105ThrArg: 3.105 ± 0.051
2.108ThrSer: 2.108 ± 0.041
2.234ThrThr: 2.234 ± 0.044
4.302ThrVal: 4.302 ± 0.059
0.574ThrTrp: 0.574 ± 0.023
1.05ThrTyr: 1.05 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
9.072ValAla: 9.072 ± 0.103
0.63ValCys: 0.63 ± 0.021
4.714ValAsp: 4.714 ± 0.063
5.077ValGlu: 5.077 ± 0.064
2.832ValPhe: 2.832 ± 0.046
5.814ValGly: 5.814 ± 0.071
1.505ValHis: 1.505 ± 0.034
4.03ValIle: 4.03 ± 0.055
1.968ValLys: 1.968 ± 0.046
7.382ValLeu: 7.382 ± 0.079
1.98ValMet: 1.98 ± 0.038
2.009ValAsn: 2.009 ± 0.035
3.529ValPro: 3.529 ± 0.057
1.921ValGln: 1.921 ± 0.041
5.58ValArg: 5.58 ± 0.074
3.648ValSer: 3.648 ± 0.056
4.205ValThr: 4.205 ± 0.056
5.718ValVal: 5.718 ± 0.069
0.901ValTrp: 0.901 ± 0.029
1.566ValTyr: 1.566 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.316TrpAla: 1.316 ± 0.027
0.14TrpCys: 0.14 ± 0.01
0.698TrpAsp: 0.698 ± 0.025
0.669TrpGlu: 0.669 ± 0.025
0.563TrpPhe: 0.563 ± 0.022
0.898TrpGly: 0.898 ± 0.027
0.347TrpHis: 0.347 ± 0.017
0.668TrpIle: 0.668 ± 0.022
0.401TrpLys: 0.401 ± 0.018
1.743TrpLeu: 1.743 ± 0.042
0.409TrpMet: 0.409 ± 0.017
0.394TrpAsn: 0.394 ± 0.017
0.804TrpPro: 0.804 ± 0.022
0.506TrpGln: 0.506 ± 0.017
1.469TrpArg: 1.469 ± 0.036
0.738TrpSer: 0.738 ± 0.024
0.733TrpThr: 0.733 ± 0.025
0.777TrpVal: 0.777 ± 0.023
0.222TrpTrp: 0.222 ± 0.013
0.284TrpTyr: 0.284 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.397TyrAla: 2.397 ± 0.045
0.254TyrCys: 0.254 ± 0.013
1.484TyrAsp: 1.484 ± 0.031
1.267TyrGlu: 1.267 ± 0.035
0.851TyrPhe: 0.851 ± 0.025
2.135TyrGly: 2.135 ± 0.054
0.491TyrHis: 0.491 ± 0.016
0.807TyrIle: 0.807 ± 0.027
0.491TyrLys: 0.491 ± 0.02
2.22TyrLeu: 2.22 ± 0.045
0.484TyrMet: 0.484 ± 0.02
0.527TyrAsn: 0.527 ± 0.02
0.993TyrPro: 0.993 ± 0.026
0.621TyrGln: 0.621 ± 0.023
2.107TyrArg: 2.107 ± 0.041
1.022TyrSer: 1.022 ± 0.029
0.954TyrThr: 0.954 ± 0.028
1.58TyrVal: 1.58 ± 0.033
0.408TyrTrp: 0.408 ± 0.018
0.624TyrTyr: 0.624 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4302 proteins (1392434 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski